AB Sciex液质联用使用说明configuring hardware

9.1 Introduction

This chapter is intended to familiarize new users of the mass spectrometer with

configuring the hardware profile with the Analyst? software.The Hardware

Configuration Editor provides the following capabilities:

?Creating, editing, and deleting hardware profiles

?Activating or deactivating hardware profiles

?Configuring devices

?Configuring devices in Simulation Mode

9.2 Materials

?Analyst? software

?AB SCIEX Mass Spectrometer

?Liquid Chromatograph

?User Manuals for all peripherals

9.3 Hardware Configuration

9.3.1 The Hardware Configuration Editor Dialog Box

You use the Hardware Configuration Editor dialog box to manage your hardware profiles so Analyst? software can communicate with attached peripheral devices.

1.Double-click on Hardware Configuration under Configure on the

Navigation bar

OR

2.On the toolbar, click the button shown in Figure9-1.

3.The Hardware Configuration Editor Dialog Box opens (Figure9-1)

Configuring Hardware

Figure 9-1Hardware Configuration Button and Window

4.This window will list all available hardware profiles.

A green check next to a profile indicates the profile is active.

A yellow circle with a blue check mark indicates that it has loaded

successfully.

A red circle with white X indicates that an error has occurred during

activation and the profile cannot be used. Refer to Activating or

Deactivating a Hardware Profile for more information.

5.New Profile: Opens the Create New Hardware Profile dialog box

(Figure9-2).

Enter a Profile Name, then click Add Device to open the Available

Devices dialog box (Figure9-3). From the Device Type drop down

menu, select each device type and corresponding device. Click OK

Configuring Hardware

Figure 9-2Create New Hardware Profile Window

6.View/Edit Profile: When a profile is deactivated, clicking the Edit Profile

button opens the Edit Profile dialog box.

Here you can add, delete, or set up devices and save your hardware profiles. Click View Profile to view the options of an active profile

7.Delete Profile: When a profile has been deactivated, clicking this button

deletes the currently selected hardware profile.

8.Activate/Deactivate Profile: Renders the currently selected hardware

profile active or inactive and deactivates the one that is active if there is one.

Figure 9-3Available Devices Window

Configuring Hardware

9.Available Devices: Opens the Available Devices dialog box. Here you can see all of the supported devices that can be configured on the workstation you are using.

10.The Analyst ? software supported devices under the chosen device type are listed as shown in Figure 9-3. Double-click on the desired device to add it to the Create New Hardware Profile window.

11.Each device must be set up individually. Highlight each device added to the profile in the Create New Hardware Profile window and click on Setup Device. For most devices, additional information or values are not required. In the Communication tab, enter the corresponding Serial Port/COM Port number for the devices being set up, e.g pumps/autosampler (Figure 9-4). The Advanced button in this window is discussed in the next section. Click OK.

12.The Mass Spec Communication is always configured as GPIB Board.

Figure 9-4Pump Configuration Window - PE200 Example

13.Verify that a Profile Name in the Create New Hardware Profile dialog box has been entered. Click OK.

14.The hardware profile name should now appear in the list and can be activated provided the communication with peripherals has been set up properly.

Note:The API 2000?, API 3200?, QTRAP ? and 3200 QTRAP ? systems have an integrated syringe pump that must be activated on the Mass Spec Configuration tab by checking the box next to “Use integrated syringe pump”, as in Figure 9-5

.

Configuring Hardware

Figure 9-5Mass Spec Configuration and Communication

9.3.2 Using Simulation Mode

Simulation mode is useful for creating batches and methods on a processing workstation. You access simulation mode through the Advanced Settings dialog box (located in the Communication tab of the Setup Device window) for each instrument or device. See Figure 9-6 and Figure 9-7to locate this button. Each device in the active hardware configuration must be configured to run in simulation mode individually.

1.This dialog box can only be edited when the current profile is deactivated. The Advanced Settings dialog box is displayed from the Configuration tab of any selected device in the profile by clicking the Advanced button.

WARNING!To prevent permanent loss of optimized instrument settings, NEVER use simulation mode on the computer workstation that is used to operate the mass

spectrometer!

Configuring Hardware

Figure 9-6Advanced Settings Window

2.Select the check box to operate the device in simulation mode. You can do this if you want to test an acquisition method and view simulated results and if you want to build a method offline. This check box is functional for all devices except the Gilson215 autosampler, Gilson233 autosampler, and Shimadzu system controller.

3.In Figure 9-7, the top picture is of the Simulations Options for the mass spectrometer. Here you will select which instrument you wish to simulate by clicking the Browse... button and navigate to the appropriate instrument file.

4.Select the appropriate Ion Source Housing from the drop-down menu

5.The Narine’s Reduction doesn’t need to be adjusted. Leave at 95%.

6.Each device will have different options under the More button

Note:It is recommended that you do not change any of the set command and polling settings in this dialog box. There are specific simulation options for the mass spectrometer, PE200 pump, Agilent1100 pump, PE200 autosampler, and

Agilent1100 autosampler – click More to access these options.

Configuring Hardware

Figure 9-7Simulation Options (the More button) Example for Mass Spectrometer and PE 200 Autosampler and Pumps