玉米雄穗分枝数与主轴长的QTL鉴定

HEREDITAS (Beijing)2007 8 ,29(8):1013?1017ISSN https://www.wendangku.net/doc/0512737106.html,

? :2006 11 27; :2007 02 07

/ :? z?MQ3 ?? ??04NG002-006-2?D =? M =η?8( ??IRT0458)? [Supported by Science and Technology Project of Sichuan Province(No.04NG002-006-2)and Program for Changjiang Scholars and Innovative Research Team in University of China(No.IRT0458)]

?#?1: (1974?),u ,? ?, Q ,? , ?? ? ?Dt M ? Tel:0835-*******;

E-mail:gaosu@https://www.wendangku.net/doc/0512737106.html,

DOI:10.1360/yc-007-1013

? ??<_ ?QTL

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Identification of QTL associated with tassel branch number and total tassel length in maize

GAO Shi-Bin,ZHAO Mao-Jun,LAN Hai,ZHANG Zhi-Ming

Maize Research Institute ,Sichuan Agricultural University ,Key laboratory of crop genetic resources and improvement for southwest ,Minis-try of Education ,Ya ’an 625014,China

Abstract :On the basis of 103SSR linkage map,QTLs associated with tassel branch number (TBN)and total tassel length

(TTL)were studied by composite interval mapping with F 3families,which were developed from the cross N87-1×9526and surveyed for phenotype under normal condition (CK)and drought-stress environment (DS).Four QTLs on chromo-somes 2,5,7,and 10,respectively,were associated with TBN under DS,two of which were not only repeatedly detected on chromosomes 5and 7under the CK but also were linked to some QTLs related to drought tolerance that had already been reported in the same mapping population.The two QTLs controlling TTL were identified on chromosomes 2and 6under CK,while three QTLs for TTL were detected on chromosomes 2,4,and 10under DS.The QTL on chromosome 2for TTL was consistent under two environments.Most of QTLs for TBN were partial additive while QTLs for TTL were dominant and over-dominant in terms of gene action .

Keywords:maize (Zea mays L.);tassel branch number;total tassel length;quantitative trait loci

? ? ? ? ? ? t( ? ? ? ?? ,3 \ ?? , ? ? γ?щ? ? 6\Λ??? ,

( [1] Geraldi ?[2]?? ??<_ ( B ?3(r =?0.65) M ? ? ? 3 \ ??$(? ?:? ?? ?

1014HEREDITAS

(Beijing)2007 29

Duvick ?[3]Y ? (Pioneer)$(?? ??\?1967 1991 ǔ 36%, ?[ / ?(??? $(? ??<_ B \? g Х ?? $(?? t( ? ? ǚт ?? 3 = ? ?,?? 9?? ǔ M Λ ? ?? ???? ? ? Schuetz [4]

* [5] ? [6]?? 3_ ? Λ= ? ? 7??<_? ? ?U ? ? ? ? 9? ? ? M? ? ? ? ?? !9 ? ? N ,Х Y ? , ?α ( B73? ?? ?[7,8] Fischer [9]Y ?h ?? ? \ ?? ? = ?\ -@ (ASI);Chapman [10]?ΛTuxpe?o sequ?n . ???? P ,? ??<_8 ,ǒP ? ? ??<_?P 3.1% Х ? ? ?? ? ? ? ?? ? ? nP = ? ? 7? ??<_ ? 2 ?QTL ǔ=3? ?U , ? ? ? Λ ? ? , ? ??? ? ? ? , ? ? ??

1 D

1.1?? ? ?

? 8?? ?j*???[11]?? , ? =3*? ? ? ( N87-1( ) 9526(т ) ǚ? .?? ,8 183 F 3Z ??? . ?Z ?? ? ? (Check,CK) (Drought Stress,DS)? 2?nP ,??? ? ? ?[12]?:?Y ??Z ? ж ? ,? P Z 5 a ?, ??? ?_ ??<_(Tassel Branch Number,TBN) $ ? (Total Tassel Length,TTL),3 ?? ? ? ? ? ? ? ?

SSR ? ? ? 8

F 2 DNA CTAB ? ??щ?? ?

??,SSR ? ? 8/ ? M?_ (MaizeGDB,https://www.wendangku.net/doc/0512737106.html,/) ?? SSR ? ?N87-1 9526 3 R ?SSR ?,? F 2 ? M SSR ?PCR E ?( ? 3щN ?\?q? 6?+? ? К ?6?u ?? $

?2 ?, E ?ǔg ?? \?? (1:2:1)? ? ? ? 8 ,ǚ? ? ?Mapmaker/Exp3.0(LOD =3.0,r =0.4),ǚ?7_ ?Kosambi ,? ?9?MapChart 2.1 1.3

QTL $ U ?

??Z ? _ QTL ǔ, ?Windows QTL Cartographer (2.0) ???? ǚ? QTLs $ ?P 2cM ǔ ,? LOD ?? ? ?Permutations ( _500 , 0.05)

2 ?

2.1? D F 3Z ? ?

? _D? _S ? F 3?Z ? ??<_ ? ? ?nP ? ( 1) ? nP ,? ?Z ? ??<_ ? ? nP , ? ??t = 3 ? N87-1? ?nP ? \ ? 9526?? , ? ? = =?? ,?:?? N87-1? 9526 > ,? F 3Z 2 ?? ? nP ? nP ,ǒ ? _ nP ? nP , ?? nP ?Z Λ? = ?? , ??= Λ ??<_ ?3? =?B ?3,ǒ ?nP 2.2

QTL $ ?U ?

?? ǚ? ? 8 ?? A 103 ??SSR ? ? $9 11 QTL ǔ,??ǔ 2 4 5 6 7D 10 (?1, 2) ??<_? nP $92 QTL ǔ??ǔ 5D 7 ;?? nP $94 QTL ǔ??ǔ 2 5 7D 10 ,3

8 ?:? ??<_ ?QTL 1015

1? c F 3v ?9 ? ? ?t

Table 1Phenotypic evaluation of tassel traits for parents and F 2:3families under two environments

? Parents F 3Z F 3families

? Trait nP Environment N87-19526 Average ￠?Range ? _

Variation coefficient

CK 9.512.513.8 4.3 27.029.0?<_(TBN)DS 7.89.312.3 3.67 2634.1CK 25.230.532.213 46.215.0 (TTL)

DS

20.4

23.9

25.3

9.5 12.3

22.7

? 5 bnlg1836 bnlg105D 7 ?bnlg1792 bnlg1305 ? ?8 $9QTL ? ?nP 3 ? ? ? nP $92 QTL ǔ,??ǔ 2D 6 ;?? nP ? $93 QTL ǔ??ǔ 2 4D 10 ,3 ǔ 2 ? ??<_QTL ǔ? ?nP ? 3

Stuber ?[13] ? 11 QTL ǔ? ?ǚ? ,6 ??<_ ǔ 4 ? ? ,>2 ? ? ǔ ?5 ? QTL ǔ ,1 ? ? U ,>?4 ? ? U ? ? $9?11 QTL ǔ? U ?, 8 ǔ? U , ? ?, Λ ? ?? ? ?? ?QTL ǔ/ ? 9526??ǔ M , 3 3 ?Λ = ? ? 3 ? U ?QTL ǔ_?? 3 ,ǒ > ? ǔ 4D 7 ?qTTL4_DS D

qTBN7_DS ? ? я ,?? 9 14.7%D

28.4%, ?? nP 8 $9?,3?U ?ǔ M/ P ?? N87-1,?? ? $9 ?3 QTL ǔ ?QTL ǔ? U ,? ,?? ?$?ǔg3 $?, ? ,ǒ? 4 ǔ?U ? ?QTL ǔ? ? я? ￠??5.6%~28.4% ,?11 QTL ǔ ? я? 10%? 5 , ?QTL ?? nP 8 $9? 7 ? ??<_qTBN7_DS ǔ

31o

? ? ?nP ,? $9 ??<_

? 2 ?11 QTL, j Stuber ?[13] ??QTL Mǚ? ,?6 ??

[4]

* ?[5]

? 3_

1 ? (? ?QTLs ?- ??

Fig.1QTLs of tassel branch number and total tassel length within SSR linkage map

1016

HEREDITAS (Beijing)2007 29

2?? ? (? QTL cJǎ ?

Table 2Putative QTL of tassel traits and their genetic parameters

Trait QTL N . N

Bin of chromosome ?

Marker interval LOD U Additive U Dominance

ǚ? Gene action *? яR 2(%)qTBN5_CK 5.01 5.02bnlg1836 bnlg105 2.3?1.270.80P 5.6qTBN7_CK 7.02 7.03bnlg1792 bnlg1305 2.3 1.63?0.57P 7.0qTBN2_DS 2.07 2.10umc1560 umc2214 3.3?1.65 1.21P 8.0qTBN5_DS 5.01 5.02bnlg1836 bnlg105 2.4?1.02?2.72OD 11.1qTBN7_DS 7.02 7.03bnlg1792 bnlg1305 3.1 3.65?7.75OD 28.4 ??<_TBN

qTBN10_DS

10.01 10.03bnlg1451 bnlg1655 3.3?2.020.45P 8.1qTTL2_CK 2.05 2.06umc2110 ucm1065 2.9?1.5 1.8OD 10.1qTTL6_CK 6.06 6.07Bnlg1443 bnlg1296 2.5?0.82?1.87OD 6.1qTTL2_DS 2.05 2.07umc2110 ucm1065 2.7?1.1 1.5OD 7.2qTTL4_DS 4.10umc1503 bnlg589 2.6 2.52 1.62P 14.7 ? TTL

qTTL10_DS

10.01 10.02

umc1318 bnlg1451

3.8

?1.5

2.8

OD

11.7

: U ? ?QTL ǔ??U M/ N87-1,= , ?U M/ т 9526 * Mǚ? ,A ( =0~0.2),P ? ( =0.21~0.80),D ( =0.81~1.2),OD ? ( >1.20)

Note:Positive values of additive indicate that the N87-1carries the allele for an increase to the value of trait,and negative values indicate that 9526contributes the allele for an increase to the value of trait.A,additive (dominant degree=0?0.2);P,partial dominant (dominant degree=0.21?0.80);D,dominat (dominant degree=0.81?1.20);OD,over-dominant (dominant degree>1.20).

? ? ??<_? ?? D ?;,1 ??<_ ?U ? D3 ? ?;,3 ?;?? ?46%~89% ,? ? ?? M 3_ ? QTL ? , ??<_ ?;?Λ ? , ? ? ? ? ?? ,? 3 \ ??<_?? , ? 7 \ ??$(? Х ?5 ? QTL ǔ ,? 1 ǔ? Mǚ? ? ,3ǖ

4 ι? ǔ,M ?? $(? ? ?

$?, Λ ? ?

?? Y ? 9 ??<_ ? ?QTL ? ( 3), 6D 8N . ?: $9 ??<_QTL Y ??,3*N . ? ? 8 $9? ??<_QTL 8?N .?bin (MaizeGDB ? j ? ?

? 10.N .? 100 щN , щN] 1 bin )?? ǚ? .8 ? N￠? ?, ? ?N .bin ?? $9 ?QTL ǔ ? QTL ? ??(Illinois low protein C B73)B73 . ? , (+8 ǔ? S =??bin 6.06~6.07 N? ???8 ǔ??? N ,(+? 2D 10N . $9 ?QTL ǔ ? ?:>?6 ?ǚ? .? ? ?QTL ǔ,8??N .K?3.bin 7??3 QTL ? ?? R , ??3* . ? R? ,???B?7 ??

?(+8 ǔ? 5D 7 ? $9? ??<_QTL ǔ?N .bin 9 9? ? ?= ??? M ,?ǔ bin 5.04?thick tassel dwarf1 M (? M?

3J ? ????t ? ?(? QTL ? ?? bin

Table 3Chromosome bin of QTL associated with TTB and TTL among other mapping populations

Trait

QTL ǔ QTL location

??<_Tassel branch number,TBN

1.01_B;1.02-1.03_C;

2.02_B;2.05_B;2.07_A/B;

3.04_C3*;3.05_B;

4.05_D;4.06_C;4.08_B/C;

5.02_C;7.0_D;7.03_A;9.04_C;10.04_C ?

Total tassel length,TTL

1.10_D;4.05_D;5.02-5.03_D;6.06-6.07_D;7.02_D

A:Illinois high oil ×Illinois low oil,Berke et al .[14];B:B73×M017,Mickelson et al .[7];C:N87-1×Zhong 31,Tang et al .[15];D:(Illinois Low Protein ×B73)×B73,Upadyayula et al .[8]

:?3.04_C3* 0, ?C ǚ? .?N .bin 3.04? $9 3 ??<_QTL

Note:As an example,3.04_C3*indicates that 3QTL associated with TBN were detected on chromosome bin 3.04with C mapping population.

8 ?:? ??<_ ?QTL 1017

? ? ???????;)[16]?=bin7.02 ? ?? M ramosa1(? M???.?? ??<_)[17],3 qTBN7_DS? ? я 9 28.4%, (+? 5D 7 8 $9? ??<_QTL3 ??

> ? ? ? ? nP $9?Λ nP R_??QTL, ?? ? ?Z Λ? ? ??t = ,? Z ?? , QTL $??; ? ?? nP $9?QTL ǔ,R_ ǚ? . ?? nP $9?3* ?3 ?? ǔ 5D7 ? ( _QTL? [11],M ?? $ ??<_ ??3??? , ?? ?? .P ? ? Ξ ??<_6\?

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