厌氧氨氧化污泥包埋固定化及其脱氮效能
第41卷第4期2015年4月北京工业大学学报JOURNAL OF BEIJING UNIVERSITY OF TECHNOLOGY Vol.41No.4Apr.2015
厌氧氨氧化污泥包埋固定化及其脱氮效能
陈光辉,李 军,邓海亮,张彦灼,郑林雪,赵白航,王昌稳,郑照明
(北京工业大学水质科学与水环境恢复工程北京市重点实验室,北京 100124)
摘 要:为维持水中厌氧氨氧化菌生物量,采用水性聚氨酯(WPU)对厌氧氨氧化污泥进行包埋固定化,同时对比聚乙烯醇(PVA)二海藻酸钠(SA)以及PVA-SA 包埋后颗粒的机械稳定性和厌氧氨氧化性能.结果显示:4种包埋颗粒均表现出良好的厌氧氨氧化性能,WPU 颗粒生物活性最好,机械稳定性最高,相比其他几种材料具有明显的优势,适合作为厌氧氨氧化包埋材料.在WPU 包埋颗粒的连续流实验中,通过不断降低水力停留时间(HRT)的方式增加容积负荷,当容积负荷为1.697kg /(m 3四d)时,WPU 包埋颗粒仍能达到80%总氮(TN)去除率,并且在100d 内没有观察到出水SS 增加和颗粒碎裂的现象,表明WPU 包埋颗粒具有很强的抗负荷冲击能力和厌氧氨氧化性能,
并且在长期运行中能保持良好的污泥截留能力和稳定性.通过16S rDNA-Cloning 分析发现:WPU 包埋颗粒内厌氧氨氧化菌主要是Candidatus Brocadia fulgida (JX243641.1),包埋材料作为载体,起到保护厌氧氨氧化菌和提高生物量的作用,但对菌体本身和菌群结构没有影响.
关键词:厌氧氨氧化;包埋固定化技术;生物脱氮
中图分类号:X 703.1
文献标志码:A 文章编号:0254-0037(2015)04-0612-09doi :10.11936/bjutxb2014070052收稿日期:2014-07-27
基金项目:国家科技重大专项资助项目(2014ZX07201-011);北京市自然科学基金资助项目(8122005);国家自然科学基金资
助项目(51308010);北京市教育委员会资助项目(KM201210005028)
作者简介:陈光辉(1986 ),男,博士研究生,主要从事污水处理与资源化方面的研究,E-mail:ghui0066@https://www.wendangku.net/doc/0f18051387.html, Nitrogen Removal Performance of Immobilized Anammox Sludge
CHEN Guang-hui,LI Jun,DENG Hai-liang,ZHANG Yan-zhuo,ZHENG Lin-xue,
ZHAO Bai-hang,WANG Chang-wen,ZHENG Zhao-ming
(Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China)Abstract :In order to maintain the anammox biomass,waterborne polyurethane (WPU)was employed as carriers to immobilize anammox sludge,as compared to polyvinyl alcohol (PVA),sodium alginate (SA)and mixture of PVA-SA .The physical stability and anaerobic ammonium oxidation activity of the granules were evaluated.The results indicate that the four immobilized granules all have higher anaerobic ammonium oxidation activity.WPU is the most suitable immobilizing material among others,because WPU granules have shown the best biological activity and physical stability.In continuous flow experiments,the physical stability and anaerobic ammonium oxidation activity of the granules were further investigated through increasing volume loading by lowering hydraulic retention time (HRT).It was found
that for WPU immobilized granules,there were no increasing effluent SS and granule fracture in the 100-day operation,and the total nitrogen (TN)removal rate still reached 80%when volume loading was 1.697kg /(m 3四d).Meanwhile WPU immobilized granules showed strong capability of retaining sludge