6种黄酮类化合物清除超氧阴离子自由基能力及其构效关系

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6种黄酮类化合物清除超氧阴离子自由基能力及其构效关系

作者：赵静李玉琴(等)

来源：《中国医药导报》2014年第29期

[摘要] 目的测定槲皮素、根皮苷、杨梅素、原花青素、葛根素和柚皮苷对超氧阴离子自

由基（O-2·）的清除能力，探讨6种化合物清除O-2·的构效关系。方法采用流动注射邻苯三酚-鲁米诺化学发光法测定6种化合物对O-2·的清除率，采用IC50来衡量样品对自由基的清除能力。以维生素C为阳性对照。结果槲皮素、根皮苷、杨梅素、柚皮苷、葛根素、原花青素对O-2·的抑制率分别为0.1547、0.2048、0.4623、0.6157、1.8098、2.9798 μmol/L。6种化合物清除O-2·能力从大到小依次为槲皮素、根皮苷、杨梅素、柚皮苷、葛根素、原花青素，均高于维生素C（4.9586 μmol/L）。结论 6种黄酮类化合物结构与清除O-2·能力密切相关：酚羟基

是其清除O-2·的必要基团，B环的羟基化程度、羟基的数目和位置，C环开环后形成的羟基和双键，A环取代基的位置、数目以及化合物的空间结构，均是影响抗氧化活性的重要因素。

[关键词] 6种黄酮类化合物；超氧阴离子自由基；清除率；构效关系

[中图分类号] O657.3 [文献标识码] A [文章编号] 1673-7210（2014）10（b）-0007-05

Study of 6 flavonoids compounds for the scavenging superoxide anion free radical ability and the structure-activity relationships

ZHAO Jing1 LI Yuqin2▲ WAN G Fangqiao2 JIA Baoxiu2 LIU Caihong2 WANG Renliang3

1.Taishan Sanatorium of Shandong Province， Shandong Province， Tai'an 271000， China；

2.School of Pharmacy， Taishan Medical University， Shandong Province， Tai'an 271016，China；

3.School of Chemical Engineering， Taishan Medical University， Shandong Province，Tai'an 271016， China

[Abstract] Objective To study the scavenging superoxide anion free radical （O-2·） ability of quercetin， myricetin， phloridzin， naringin， puerarin and proanthocyanidins， and to explore their structure-activity relationships for removing O-2·. Methods The scavenging O-2· ability was determined by flow injection analysis-pyrogallol-chemiluminescence. IC50 was calculated and used to examine the scavenging free radical ability. Vitamin C was used as positive control. Results The results showed that IC50 of quercetin， myricetin， phloridzin， naringin， puerarin and proanthocyanidins were 0.1547， 0.2048， 0.4623， 0.6157， 1.8098，2.9798 μmol/L，respectively. The scavenging O-2· ability of the 6 compounds were arranged as quercetin，myricetin， phloridzin， naringin， puerarin and proanthocyanidins， and all were higher than Vitamin C （4.9586 μmol/L）. Conclusion The scavenging O-2· ability of 6 flavonoids compounds