The histone acetylation mediated by Gcn5 regulates the Hoxc11 gene expression in MEFs

The histone acetylation mediated by Gcn5 regulates the Hoxc11 gene expression in MEFs

Ji Hoon Oh;Ji-Yeon Lee;Kyoung-Ah Kong;Jie Min Kim;Myoung Hee Kim

【期刊名称】《生物化学与生物物理学报：英文版》

【年(卷),期】2017(049)007

【摘要】Hox genes are responsible for encoding transcription factors that are essential for anterior-posterior body patterning at early stages of embryogenesis.However,detailed mechanisms of Hox genes are yet to be defined.Protein kinase B alpha (Akt1) was previously identified as a possible upstream regulator of Hoxgenes.Furthermore,the Hoxc11 gene has been upregulated in Akt1 null (Akt1-/-) mouse embryonic fibroblasts (MEFs),while repressed in wild-type MEFs.In this study,we propose to investigate the role of Gcn5,a histone acetyltransferase,in the regulation of Hoxc11 expression in MEFs.We showed that the H3 lysine 9 acetylation (H3K9ac) status has the same correlation with Hoxc11 expression and reported that Gcn5 is associated with the upregulation of Hoxc11 expression through H3K9ac in Akt1-/-MEFs.Since Hoxc11 was upregulated through histone acetylation in Akt1-/-MEFs,a functional role of Gcn5 on Hoxc11 expression was analyzed in Akt1-/-MEFs treated with Gcn5 specific inhibitor or transfected with Gcn5-small interfering RNA (Gcn5-siRNA).When the expression of Hoxc11 was analyzed using RT-PCR and real-time