PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATO

PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN

FETAL HEPATOCYTES PRETREATED BY SALVIA

MILTIORRHIZA BUNGE

赵英仁;朱风群;和水祥;苌新明;乔文

【期刊名称】《西安医科大学学报(英文版)》

【年(卷),期】2003(015)002

【摘要】Objective The effects of salvia miltiorrhiza bunge (SMB) on collagen synthesis in the human fetal hepatocytes culture were studied. Methods The collagen synthesis of hepatocytes were stimulated by the addition of carbon tetrachloride (CCl4) to the culture medium, the concentration of type procollagen (PC) in the culture medium and the hydroxyproline (Hyp) in hepatocytes were determined, as well as the activity of se-dependent glutathione peroxidase (Se-GSH-Px) and the concentration of malondiadehyde (MDA) in the culture medium. Results A significant decrease in PC, Hyp and MDA production, and the significant increase in Se-GSH-Px activity were observed in the cultures pretreated with 1 g L-1 SMB for 4 hours compared with the untreated cultures. Analysis of the Se-GSH-Px/MDA ratio in SMB pretreated group showed more marked increase compared to that of the untreated group (P<0.01). There was a significant negative correlation between the ratio of Se-GSH-Px/MDA and the concentration of PC in SMB pretreated