Chemokines regulate the migration of Neural Progenitor sites of neuroinflammation

Development/Plasticity/Repair

Chemokines Regulate the Migration of Neural Progenitors to Sites of Neuroinflammation

Abdelhak Belmadani,*Phuong B.Tran,*Dongjun Ren,and Richard https://www.wendangku.net/doc/38628553.html,ler

Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School,Chicago,Illinois60611

Many studies have shown that transplanted or endogenous neural progenitor cells will migrate toward damaged areas of the brain. However,the mechanism underlying this effect is not clear.Here we report that,using hippocampal slice cultures,grafted neural progenitor cells(NPs)migrate toward areas of neuroinflammation and that chemokines are a major regulator of this process.Migration of NPs was observed after injecting an inflammatory stimulus into the area of the fimbria and transplanting enhanced green fluorescent protein(EGFP)-labeled NPs into the dentate gyrus of cultured hippocampal slices.Three to7d after transplantation,EGFP–NPs in control slices showed little tendency to migrate and had differentiated into neurons and glia.In contrast,in slices injected with inflam-matory stimuli,EGFP–NPs migrated toward the site of the injection.NPs in these slices also survived less well.The inflammatory stimuli used were a combination of the cytokines tumor necrosis factor-?and interferon-?,the bacterial toxin lipopolysaccharide,the human immunodeficiency virus-1coat protein glycoprotein120,or a?-amyloid-expressing adenovirus.We showed that these inflammatory stimuli increased the synthesis of numerous chemokines and cytokines by hippocampal slices.When EGFP–NPs from CC chemokine receptor CCR2knock-out mice were transplanted into slices,they exhibited little migration toward sites of inflammation.Similarly, wild-type EGFP–NPs exhibited little migration toward inflammatory sites when transplanted into slices prepared from monocyte che-moattractant protein-1(MCP-1)knock-out mice.These data indicate that factors secreted by sites of neuroinflammation are attractive to neural progenitors and suggest that chemokines such as MCP-1play an important role in this process.

Key words:CCR2receptors;MCP-1;migration;neural progenitors;neurogenesis;neuroinflammation;neural precursor

Introduction

The use of neural progenitors(NPs)for repairing brain damage is a subject of great interest(Gage,2002;Parent,2003;Lindvall et al.,2004),holding out the hope of actually reversing,rather than merely attenuating,neurodegeneration associated with many brain disorders(Fricker et al.,1999;Aboody et al.,2000;Picard-Riera et al.,2002;Iwai et al.,2003;Imitola et al.,2004;Jin et al., 2004;Kelly et al.,2004;Pluchino et al.,2005).To achieve this aim, neural progenitors must migrate to regions of brain injury,dif-ferentiate into cells with the correct phenotype,and integrate appropriately into a neuronal circuits(Abrous et al.,2005).The potential of neural progenitors for performing these tasks has been demonstrated in cell culture and in vivo using animal mod-els of brain disease(Gage,2002;Abrous et al.,2005).However, our understanding as to how all of these processes occur and how they can be manipulated to therapeutic advantage is incomplete. It has frequently been demonstrated that neural progenitors transplanted into the brain will migrate toward either localized (e.g.,stroke)or diffuse(e.g.,demyelinated)areas of brain damage (Fricker et al.,1999;Aboody et al.,2000;Arvidsson et al.,2002; Ehtesham et al.,2002;Iwai et al.,2003;Yip et al.,2003;Glass et al., 2005).These observations suggest that factors associated with damaged areas of the brain can direct the migration of progeni-tors.Infection of the brain,trauma,neurodegeneration,or other types of brain injury usually result in a neuroinflammatory re-sponse involving components of the innate immune system of the brain,including the activation of astrocytes and microglia (Huang et al.,2000;Aarum et al.,2003;DeLeo et al.,2004).One consequence of this response is the upregulation of cytokine and chemokine synthesis by these activated cells(Huang et al.,2001; Babcock et al.,2003).Chemokines are small secreted proteins that have been shown to play a key role in the organization of leukocyte migration under normal conditions as well as during neuroinflammatory responses(Huang et al.,2000,2001;Tran and Miller,2003).Recently,chemokines have been shown to play a role in directing the migration of neural progenitors in the developing brain(Zou et al.,1998;Lu et al.,2002;Stumm et al., 2003;Tran and Miller,2003)and peripheral nervous system(Bel-madani et al.,2005).We demonstrated that neural progenitors prepared from the postnatal brain express numerous chemokine receptors,and that neural progenitors in neurogenic regions of the brain normally express these receptors(Tran et al.,2004a). We therefore hypothesized that chemokines released from sites of neuroinflammation might help to guide the migration of neu-ral progenitors to damaged areas of the brain.

Received July29,2005;revised Feb.5,2006;accepted Feb.7,2006.

This work was supported by National Institutes of Health Grants R01NS043095,R01DA013141,and R37MH040165.WethankDr.WilliamJ.KarpusforprovidinguswithCCR2andMCP-1KOmice,Dr.JaimeGrutzendler for CXCR3–EGFP mice,Dr.Warren Tourtellotte for the EGFP adenovirus,and Dr.Tsuneya Ikezu for the?-amyloid–GFP adenovirus.A special thank you goes to Dr.Peter Toth for his technical assistance with MetaMorph software. *A.B.and P.B.T.contributed equally to this work.

Correspondence should be addressed to Prof.Richard https://www.wendangku.net/doc/38628553.html,ler,Department of Molecular Pharmacology and Biological Chemistry,Northwestern University,303East Chicago Avenue,Ward8-296,Chicago,IL60611.E-mail: r-miller10@https://www.wendangku.net/doc/38628553.html,.

DOI:10.1523/JNEUROSCI.0156-06.2006

Copyright?2006Society for Neuroscience0270-6474/06/263182-10$15.00/0

3182?The Journal of Neuroscience,March22,2006?26(12):3182–3191

Materials and Methods

Animals.CD1(ICR;Charles River Laboratories,Wilmington,MA),B6?

129,C57BL/6J(The Jackson Laboratory,Bar Harbor,ME),C57BL/6J

mutant mice and BALB/c CX3CR1–enhanced green fluorescent protein

(EGFP)were used in these experiments,and all animal experimentation

protocols were approved by the Northwestern University Animal Care

and Use Committee.Mice lacking the CC chemokine receptor CCR2,i.e.,

CCR2?/?from the B6?129strain and monocyte chemoattractant

protein-1(MCP-1)[i.e.,MCP-1?/?from the(129Sv/J?C57BL/6)F1

strain],were generous gifts from Dr.William J.Karpus(Northwestern

University,Chicago,IL)and have been characterized previously(Kuziel

et al.,1997;Lu et al.,1998).Heterozygous CX3CR1GFP/?mice were a

generous gift from Dr.Jaime Grutzendler(Northwestern University),

and their phenotype has been described previously(Jung et al.,2000).

Preparation of organotypic hippocampal slice culture.Seven-day-old

CD1mice were killed by decapitation,and the brains and meninges were

removed under aseptic conditions,followed by separation of the hip-

pocampus from the two hemispheres.As described by Belmadani et al.

(2001),the hippocampal tissue blocks were cut by a McIlwain tissue

chopper into350-?m-thick coronal slices.The slices(three to four)were placed on semiporous membrane inserts(Millicell-CM,0.4?m;Milli-pore,Bedford,MA)and transferred to six-well culture plate with1.2ml

of MEM supplemented with25%horse serum(Invitrogen,Carlsbad,

CA), 6.5mg/ml D-glucose(Sigma,St.Louis,MO),and0.5m M L-glutamine.After3d in cultures,the medium was changed to serum-free Neurobasal medium(Invitrogen)with2%B27supplement(Invitro-

gen),6.5mg/ml D-glucose,and0.5m M L-glutamine with subsequent

medium change twice a week.In other experiments,slices were prepared

from MCP-1mutant mice,CXCR3–EGFP,mice or B6?129PF2/J mice

as controls for CCR2mutant mice.

Propagation of mouse neurospheres and micrografting.Subventricular

zone(SVZ)blocks were dissected from5-to7-d-old CD1mice,dissoci-

ated via repeated trituration,and grown in flasks in medium containing

Neurobasal/B27/N2media supplemented with heparin(2?g/ml; Sigma),epidermal growth factor(10ng/ml;Sigma),or basic fibroblast growth factor(10ng/ml;BD Biosciences,Franklin Lakes,NJ),as de-scribed by Tran et al.(2004a).After7d in cultures,the resulting neuro-spheres were dissociated into single cells that were cultured for an addi-tional7d to form secondary neurospheres.They were then transfected with a green fluorescent protein vector(gift from Dr.Warren Tourtel-lotte,Northwestern University),and EGFP expression of neurospheres was routinely checked using fluorescent microscope.After3–4d in cul-tures,green neurospheres were enzymatically dissociated(25mg/ml trypsin/EDTA),counted,and loaded on the tip of a0.5?l Hamilton syringe mounted on a micromanipulator.Suspensions of these cells(3–4000cells/0.2?l)were seeded locally onto the culture in the area of the dentate gyrus(DG)as indicated in Figure1.After3–7d in culture, grafted slices were analyzed by confocal microscopy for cell migration or processed for immunostaining.In some experiments,dissociated neuro-spheres were labeled with PKH26dye(red fluorescent linker;Sigma) before transplantation.In other experiments,neurospheres were gener-ated from the SVZ of CCR2mutant mice.

Neuroinflammation.A neuroinflammatory response was achieved by

injecting0.1–0.2?l of solution of an inflammatory stimulus(or control solution)into the area of the fimbria of cultured hippocampal slices using a0.5?l Hamilton syringe mounted on a micromanipulator.The inflammatory stimuli used were a combination of the cytokines tumor necrosis factor-?(TNF-?)and interferon-?(IFN-?)(20ng each),the bacterial toxin lipopolysaccharide(LPS)(10?M),the human immuno-deficiency virus-1(HIV-1)coat protein glycoprotein120(gp120)(250 p M),or a recombinant adenovirus coexpressing EGFP and?-amyloid(3 n M)(Ikezu et al.,2003).In the controls,we used injection of saline solution of comparable volume(0.1–0.2?l).Of note,injections of0.1–0.2?l were performed using a0.5?l Hamilton syringe operated by a manual micrometric stage mounted on a microscope under a10?objective.

Quantification of cell migration.After transplantation of labeled cells,

the cultures were incubated for24h to settle,imaged,further incubated for3,7,and15d,and then fixed in paraformaldehyde(4%).They were rinsed in PBS,mounted,and examined using confocal microscopy.Con-focal images were further processed for cell migration using MetaMorph software(Universal Imaging Corporation,Downington,Chester,PA). The average distance between the site of injection and25–50green(or red)cells was calculated in each slice and expressed as a migratory dis-tance(MD)in micrometers.

Immunohistochemistry.Fixed cultures(slices and grafted cells)were washed in PBS,permeabilized with0.1%Triton X-100(Sigma),and incubated with primary antibodies diluted in blocking solution over-night at4°C.The following primary antibodies were used:glial fibrillary acidic protein(GFAP)(mouse IgG,1:300;Sigma),nestin(mouse IgG, 1:300;PharMingen,San Diego,CA),and neuronal-specific nuclear pro-tein(NeuN)(mouse IgG1,1:300).A combination of CD11b(mouse IgG1,1:400;PharMingen)and F4/80(rat IgG2b,1:50;Caltag,South San Francisco,CA)antibodies was also used to stain for macrophages and microglia.After washing in blocking solution,the appropriate isotype-specific secondary antibodies labeled with Alexa Fluor488-,Alexa Fluor 633-,and Alexa Fluor543-conjugated preparations(1:500;Invitrogen) were applied,and the cultures were incubated for1h.After repeated washing in PBS,cultures were mounted using Vectashield containing 4?,6?-diamidino-2-phenylindole(DAPI)(Vector Laboratories,Burlin-game,CA)and analyzed using a confocal microscope.

To examine colocalized expression patterns,two-color confocal imag-ing was performed with an Olympus Optical(Melville,NY)Fluoview laser scanning confocal microscope system(FV500;Olympus Optical). EGFP-transfected cells were excited at488nm using an argon laser,and Alexa Fluor-543-and Alexa Fluor-633-tagged secondary antibodies were visualized by excitation at543and633nm with a helium–neon laser. Images were acquired sequentially in the appropriate channels.Optical sections(0.175?m)were collected as stacked z-dimension images.The z-dimension reconstructions were inspected orthogonally in both the horizontal and vertical planes.Orthogonal planes were obtained off-line using the MetaMorph image analysis software.Cells were considered as double labeled if three independent researchers judged colocalization from all observation angles using the off-line version of MetaMorph. Dendritic structures growing deep into the tissue were visualized in ex-tended focus z-projection images.To determine the number of trans-planted cells becoming glia or neurons,cultured slices were fixed and processed for immunohistochemistry.Confocal images of EGFP expres-sion and DAPI staining were obtained using a Zeiss(Oberkochen,Ger-many)510Meta confocal microscope with a UV laser and merged to show DAPI–EGFP-expressing cells.An average of20–25DAPI–GFP-labeled cells per slice were analyzed for neuronal and glial differentiation, and the proportion of GFAP-or NeuN-positive cells was determined as a percentage?SEM of DAPI-positive cells.

Quantification of cell survival of grafted cells.Fixed cultures were pro-cessed directly for measurement of the intensity of EGFP fluorescence using MetaMorph software.Some cultures were used for the detection and the quantification of cell death using In Situ Cell Detection kit, fluorescein,or tetramethylrhodamine red[terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling(TUNEL)as-say]following the instructions of the manufacturer(Roche,Indianapo-lis,IN).

Quantification of chemokine concentrations.Culture medium(25?l) from control and treated slices were used to measure chemokine and cytokine levels by a LINCO plex immunoassay(LINCO Research,St. Charles,MI).Briefly,The assay is based on the Luminex100IS technol-ogy and uses color-coded microparticles to which are coupled antibodies specific to the cytokines/chemokines of interest.Microparticles,stan-dards,and samples are pipetted into wells of a98-well plate,and the specific antibodies captures the analytes(cytokines/chemokines)of in-terest.Biotinylated antibodies specific to the analytes are added and form an antibody–antigen sandwich.Streptavidin–phycoerythrin(PE)are added to generate a signal.The microparticles are read using a Luminex 100IS dual-laser analyzer:one laser classifies the microparticle and de-termines the analyte that is being detected,and the second laser deter-mines the magnitude of the PE-derived signal that is in direct proportion to the amount of analyte bound.

Belmadani et al.?Chemokines and Neural Progenitor Migration J.Neurosci.,March22,2006?26(12):3182–3191?3183

Statistical analysis.Results were analyzed with ANOVA with Bonferroni’s post hoc tests.p values ?0.05were considered significant.The number of different experiment (n )and the number of slices used per group and per exper-iment are indicated for each experiment.The number of cells counted per slice is indicated above.

Results

Differentiation of postnatal neural progenitors in cultured mouse hippocampal slices The paradigm that we used in these studies was to examine the migration and devel-opment of NPs transplanted into the area of the DG of mouse hippocampal slice cul-tures (MHSCs)(CD1strain)(supplemen-tal Fig.S1A ,available at https://www.wendangku.net/doc/38628553.html, as supplemental material).We used NPs isolated from the SVZ of wild-type (WT)mice (5-to 7-d-old)and grown as neurospheres.Neurospheres can be la-beled with EGFP using an adenoviral vec-tor (supplemental Fig.S1C ,available at https://www.wendangku.net/doc/38628553.html, as supplemental mate-rial).Alternatively,cells can be labeled

with PKH26,a red fluorescent lipophilic dye that binds irreversibly to cell membranes (supplemental Fig.S1C ?,available at https://www.wendangku.net/doc/38628553.html, as supplemental material).The neurospheres can be enzymatically dissoci-ated into single cells and used as EGFP–NPs or PKH26–NPs for transplantation purposes.

Mouse hippocampal slice cultures were prepared as described by Belmadani et al.(2001).These slices can be grown for sev-eral weeks while retaining their basic cellu-lar organization and intrinsic connections (supplemental Fig.S1A ,available at https://www.wendangku.net/doc/38628553.html, as supplemental material).We microinjected suspensions of labeled

WT NPs (3–4000cells/0.2?l)into the area of the DG of MHSCs (supplemental Fig.S1,available at https://www.wendangku.net/doc/38628553.html, as sup-plemental material).Although many cells were washed away during this procedure,numerous green fluorescent cells were

found to be stably integrated into the slices when examined sev-eral hours or days later.Initially,these cells maintained the spher-ical morphology of dissociated progenitor cells (Fig.1A ,B )and

expressed the progenitor cell marker nestin (Fig.2).However,

after 3–7d,there was a dramatic change in their morphology.

Most cells developed a highly differentiated morphology resem-bling neurons or glia (Fig.1C ).We further examined the pheno-type of these cells by staining slices with markers specific for

neurons (Fig.3,NeuN)or glia (Fig.4,GFAP).

Migration and differentiation of neural progenitor cells in

response to inflammation

To test the migratory ability of NPs in response to neuroinflam-mation,we attempted to produce a neuroinflammatory response

within 7-d-old MHSCs.To do this,we injected an inflammatory

stimulus,such as a combination of TNF-?–IFN-?,into the area of the fimbria of the slice.Sixteen hours later,?3–4000EGFP-expressing progenitor cells were injected into the DG of the slice (schematic green spots in supplemental Fig.S1B ,available at https://www.wendangku.net/doc/38628553.html, as supplemental material ).In preliminary ex-periments,we tested the accuracy of the injections by using an EGFP-expressing adenoviral vector that indicated the local na-ture of the injections (Fig.5A ).We examined the position and phenotypes of cells 3–5d after progenitor cell injection.In control slices (no cytokines,saline injection),grafted cells remained in the vicinity of the DG injec-tion site (Fig.5B ).In contrast,in MHSC cultures that had been injected with TNF-?–IFN-?for 16h,many EGFP-labeled pro-genitors had migrated away from the DG into the parenchyma of the slice toward the site of cytokine injection (Fig.5C ).In

both Figure 1.Developmental potential of NPs expressing EGFP (green cells).A ,Confocal image of EGFP–NPs transplanted into the area of the dentate gyrus (dg)of a cultured mouse hippocampal slice after 18h in culture,illustrating the spherical morphology of cells,similar to that of dissociated neurospheres (as illustrated in C ).Scale bar,500?m.A is magnified in B .Scale bar,100?m.C ,Confocal image showing grafted EGFP–NPs have differentiated into cells with neuronal and glial morphology after 3–5d in

cultures.Figure 2.Undifferentiated phenotype typical of EGFP–NPs 48h after transplantation into the dentate gyrus of 7-d-old hip-pocampal slice cultures.A ,B ,Double-fluorescent confocal images of neural progenitor cells:EGFP (A ,green)and anti-nestin (B ,red).C ,DNA was visualized by staining with DAPI.D ,Three-dimensional analysis of a double-fluorescent confocal image of an NP:EGFP (green)and anti-nestin (red).The x–z and y–z images are shown.n ?6and 9slices per experiment were used.Scale bar,50?

m.Figure3.ConfocalimagesshowingneuronaldifferentiationofgraftedNPsaftertransplantationofEGFP–NPsintothedentate gyrus of 7-d-old hippocampal slice cultures.After 3–7d,some grafted EGFP–NPs (green,arrowhead in A )stained for NeuN (red,arrowhead in B and in merged image,yellow,in C ).C ,Three-dimensional analysis of double-fluorescent confocal image of NPs:EGFP (green)and anti-NeuN (red).In control slices,9of 230EGFP-labeled cells were NeuN positive and 3of 98EGFP cells were NeuN positive in TNF-?–IFN-?-injected slices.n ?3,6slices per group per experiment.Scale bars,20?m.3184?J.Neurosci.,March 22,2006?26(12):3182–3191Belmadani et al.?Chemokines and Neural Progenitor Migration

groups,many of the cells costained for the astrocytic marker GFAP (Fig.4),whereas relatively few stained for the neuronal marker NeuN (Fig.3)(Morrow et al.,2001).Some grafted cells

did not show any detectable neuronal or glial phenotype and

remained poorly differentiated.We also observed that the fluo-rescence of grafted cells that had been in culture for 1week or

more appeared to be less pronounced in cytokine-treated slices.

Indeed,we found that the average fluorescence intensity of

grafted cells was reduced in cytokine-injected slices compared

with controls (Fig.6D ).We investigated whether cell damage,

death,or leakage of EGFP could explain this decrease in cell flu-orescence.We used the TUNEL assay to measure cell death and

observed that there were increased numbers of TUNEL-positive

EGFP-expressing progenitor cells in cytokine-injected slices (Fig.

6A–C ,arrows).Interestingly,however,EGFP cells with dimmer

fluorescence were not all TUNEL positive (Fig.6A–C ,arrow-heads),suggesting that EGFP synthesis may also be downregu-lated in the face of the neuroinflammatory microenvironment of

the slice.When PKH26-labeled NPs were used in cytokine-injected slices (Fig.6A ?,C ?),an increase in TUNEL response was

also observed (Fig.6B ?,C ?),although no significant decrease in

PKH26cell membrane fluorescence was noted (Fig.6).Together,

these data indicate that,while migrating,some grafted cells die

(increase in TUNEL response),whereas others undergo changes

in protein synthesis (decrease in EGFP fluorescence)(Fig.6D ,

with no change in PKH26cell membrane fluorescence).

The migratory capacity of NPs in the face of neuroinflamma-tion was further examined using three other inflammatory stim-uli in addition to TNF-?–IFN-?(Fig.7A ,B ).We used HIV-1–

gp120,the coat protein of HIV-1that is known to be a potent neurotoxin (Fig.7A ?,B ?),LPS,an activator of Toll 4receptors (Fig.7A ?,B ?),and ?-amyloid,which is involved in the patho-genesis of Alzheimer’s disease (Fig.7A ?,B ?,C ?).The ?-amyloid was expressed in the slices using a ?-amyloid–EGFP-expressing adenovirus.These treatments were performed 16h before transplanta-tion of progenitor cells.After treatment with each reagent over a period of 3d,many cells migrated away from their orig-inal transplantation site toward the site of the inflammatory stimulus.The degree to which this occurred depended on the stim-ulus used (Fig.7).After 72h transplanta-tion,in the LPS-injected group,84%of the

slices exhibited migratory cells with a mean MD of 273?23?m.In TNF-?–IFN-?-injected slices,75%of the slices showed migratory responses,with an MD of 181?54?m,and,in HIV-1–gp120-injected slices,23%of the slices showed migration,with an MD of 123?86?m.In the experiment using the ?-amy-loid–EGFP adenovirus,the extent of ?-amyloid expression was clearly indi-cated from the expression of EGFP.The major cell type expressing the ?-amyloid appeared to be astrocytes.In these stud-ies,the neural progenitors we trans-planted were stained red using the mem-brane-soluble PKH26dye.As can be observed in Figure 7,A ?,B ?,and C ?,in

these slices,we were able to demonstrate clear migration of red cells toward the green area of ?-amyloid expression (n ?2).The role of chemokines in the migration of progenitor cells toward sites of neuroinflammation The experiments described above indicate that neural progeni-tors will migrate toward the injection site of different agents ca-pable of inducing a neuroinflammatory response.In the CNS,the innate immune response involves the activation of astrocytes and microglia,resulting in the synthesis of a cascade of proinflamma-tory molecules,frequently initiated by TNF-?and followed by other cytokines and numerous chemokines (Huang et al.,2000;DeLeo et al.,2004).To demonstrate that this cascade of events occurred in these studies,we conducted experiments to demon-strate the activation of microglia in response to an inflammatory stimulus (i.e.,TNF-?–IFN-?)in the cultured slices.We cultured hippocampal brain slices from CX3CR 1–EGFP transgenic mice (Fig.8A ,B ).The CX3CR 1chemokine receptor is the receptor for the chemokine fractalkine and is predominantly expressed in mi-croglial cells.In the present context,it provides a useful marker for these cells whose distribution is well described by the expres-sion of EGFP (Fig.8A )(Davalos et al.,2005).We observed that,after the injection of TNF-?–IFN-?at various points in these slices,the area around the injection site was characterized by many EGFP-expressing microglia (Fig.8B ).We then immuno-stained for microglia/macrophage markers in slices that were in-jected with either TNF-?–IFN-?(Fig.8A ?,B ?,red)or an adeno-virus expressing EGFP–?-amyloid (Fig.8A ?,B ?,C ?,red)as inflammatory stimuli.We showed that staining for CD11b (mi-croglia)and F4/80(macrophages)was concentrated around

the

Figure 4.ConfocalimagesshowingastrocytedifferentiationofgraftedNPsaftertransplantationofEGFP–NPsintothedentategyrus

of7-d-oldhippocampalslicecultures.After3–7d,somegraftedEGFP–NPs(green,arrowheadin A )stainedforGFAP(red,arrowheadin B and in merged image,yellow,in C ).C ,Three-dimensional analysis of a double-fluorescent confocal image of NPs:EGFP (green)and anti-GFAP (red).The x–z and y–z images are shown.One hundred fifty-five of 253EGFP cells were GFAP positive (61?5%)in control slices,and106of138EGFPcellswereGFAPpositive(77?7%)inTNF-?–IFN-?-injectedslices.n ?3,6slices/pergroupperexperiment.Scalebar,50?

m.Figure 5.PatternsofmigrationofEGFP–NPs3–5dafterinjectionofaninflammatorystimulusintotheareaofthefimbria(reddotin C ),followedbytransplantationofEGFP–NPs(greendotin B and C )intothedentategyrusof7-d-oldhippocampalslicecultures.Confocal images were further processed for assessment of cell migration using MetaMorph software,and the extent of cell migration was deter-mined as the average distance between the site of injection and 25–50green cells in each slice as shown in B and C .The accuracy of the injectionisindicatedbythegreenspotin A ,whichillustratesEGFPexpressionaftertheinjectionofanEGFP-adenovirusintotheslice.Scale

bar,500?m.

Belmadani et al.?Chemokines and Neural Progenitor Migration J.Neurosci.,March 22,2006?26(12):3182–3191?3185

area of the inflammatory site (Fig.8).We then conducted an experiment in which WT–EGFP–NPs were grafted onto WT slices (all from CD1mice)that were in-jected with TNF ?–IFN-?.First,we found that endogenous CD11b ?/F4/80?micro-glia and macrophages were present at the site of the inflammatory stimulus and dis-played small round or spindle shapes (Fig.8A ?,B ?D ?,E ?).Second,grafted EGFP-expressing cells migrated toward the site of the inflammatory stimulus (Fig.

8B ?,C ?,E ?,green).Third,no CD11b ?

/F4/80?macrophage cells colocalized with EGFP cells (Fig.8E ?,merged image).This indicates that EGFP-expressing cells ob-served at inflammatory sites were actually transplanted EGFP–NPs that had mi-grated in response to inflammation and not migrating macrophages that had taken up fluorescent debris.

Activation of microglia and astrocytes should be accompanied by upregulation of chemokine synthesis.Indeed,we observed a several fold increase in chemokine levels [e.g.,MCP-1,interferon-?inducible pro-tein of 10kDa (IP-10),RANTES (regu-lated on activation,normal T-cell ex-pressed and secreted),and KC (the mouse growth-related oncogene GRO-?)and also in levels of the cytokine interleukin-6(IL-6),in slices treated with TNF-?–IFN-?(Table 1).Similar increases were observed with HIV-1–gp120,LPS,and ?-amyloid–EGFP (supplemental Table 1,

available at https://www.wendangku.net/doc/38628553.html, as supple-mental material).Of importance,TNF-?–IFN-?and LPS evoked the largest stimula-tion of chemokine synthesis,which may explain their increased ability to produce migratory responses (Fig.7).Of the nu-merous chemokines,we noted that the chemokine MCP-1/CCL2is often highly upregulated in association with neuroin-flammatory responses (Huang et al.,2000;Huang et al.,2001;Thibeault et al.,2001).MCP-1has been shown to be responsible for the migration of leukocytes into the

brain under different conditions.For example,synthesis of

MCP-1by astrocytes and microglia in the brain is primarily re-sponsible for the influx of leukocytes that occurs after axonal

damage in the brain or in association with neuroinflammatory

disease such as experimental autoimmune encephalomyelitis

(EAE),a rodent model of multiple sclerosis (Huang et al.,2001;

Babcock et al.,2003).This stimulated us to conduct experiments

examining the migration of neural progenitor cells using CCR 2

(B6?129strain)and MCP-1[(129Sv/J ?C57BL/6)F 1strain]

mutant mice.We prepared wild-type neurospheres from B6?

129mice and transplanted them into cultured slices from this

strain.As in the case of CD1mice (see above),in control slices

(saline injection),transplanted cells remained in the vicinity of

the DG injection site.In slices that were injected with TNF-?–IFN-?,cells migrated toward the inflammatory stimulus.We then prepared neurospheres from CCR 2knock-out (KO)mice (B6?129strain)and transplanted them into hippocampal slices from the same strain.The initial engraftment of these cells ap-peared indistinguishable from wild-type cells (Fig.9A ),most grafted NPs remaining confined to the site of transplantation.However,the behavior of the cells after treatment of slices with TNF-?–IFN-?was different,the degree of migration observed being greatly reduced (Fig.9A ,B ).These results suggest that ac-tivation of CCR 2receptors expressed by neural progenitors may be important in guiding their migration.We therefore conducted additional experiments in which we transplanted neural progen-itors from wild-type mice,but the hippocampal slice cultures were taken from MCP-1KO mice.In this case,we also observed a much reduced degree of migration of the transplanted progen-itors toward the site of TNF-?–IFN-?injection (Fig.9A ?,B ?

).

Figure 6.Survival of grafted migrating neural progenitors in response to TNF-?–IFN-?injection into hippocampal slice cultures.TUNEL assays for grafted migrating NPs (red in B ,C and green in B ?,C ?)were used to assess cell survival.Arrowhead in A indicates an EGFP–NP cell with reduced fluorescence that is TUNEL negative (arrowhead in B and C ).Arrows in A indicate EGFP–NPs that are TUNEL positive (arrows in B ,red and in merged image,yellow in C ).In cytokine-injected slices,70of 167cells (42?10%)were TUNEL positives versus 26of 194cells (13?2.5%)in control slices (p ?0.01;n ?2and 10–11slices per

group).Scale bar,50?m.Confocal images in A ?,B ?,and C ?show migrating PKH26–NPs (red cells)3d after injection of

TNF-?–IFN-?into the area of the fimbria,after transplantation of PKH26–NPs into the dentate gyrus of cultured slices.Images in A ?and B ?weremergedtoshowdeadanddyingcells(arrowheadsin C ?).Scalebars,20?m.Arrowheadsin A ?,B ?,and C ?show PKH26-labeled NPs (A ?,C ?)and TUNEL staining (B ?,C ?).In cytokine-injected slices,46of 114cells were TUNEL positive compare with 23of 175cells in control slices (p ?0.0001;n ?9slices per group).Quantification of the average fluorescence of cells (D )(*p ?0.0001;n ?3and 17slices per group)was generated from images such as those shown in A using MetaMorph software.When PKH26-labeled cells were used,the average fluorescence in both groups was in the range 339?15(arbitrary fluorescence units).3186?J.Neurosci.,March 22,2006?26(12):3182–3191Belmadani et al.?Chemokines and Neural Progenitor Migration

Thus,these results indicate that MCP-1is primarily responsible for the migration of progenitors under these neuroinflammatory conditions.

Finally,we asked whether the increased TUNEL response to neuroinflammatory stimuli was also lost with nonmigrating CCR 2mutant cells.We found that there were very few TUNEL-positive cells in both TNF-?–IFN-?-treated slices and control slices (Fig.10),suggesting that both the chemo-tactic and the cell survival responses may be dependent on CCR 2-mediated migration.

Discussion

The experiments described in this paper allow us to make two primary conclusions.First,neural progenitors will migrate to-

ward sites of neuroinflammation,and,second,this may be attrib-utable to,at least in part,the release of chemokines from activated cells participating in the neuroinflammatory response.That this should be so is consistent with several previous observations in the literature.These include observations that neural progenitors will migrate toward areas of brain injury in vivo (Picard-Riera et al.,2002;Ben-Hur et al.,2003;Kokaia and Lindvall,2003;Parent,2003;Imitola et al.,2004;Jin et al.,2004;Kelly et al.,2004;Glass et al.,2005;Pluchino et al.,2005),that neural progenitors express chemokine receptors (Ji et al.,2004;Krathwohl and Kaiser,2004b;Peng et al.,2004;Tran et al.,2004a;Pluchino et al.,2005),that chemokines act as chemoattractants for these cells (Tran et al.,2004a;Widera et al.,2004;Pluchino et al.,2005),and that cells involved in neuroinflammatory responses are known to be sources of chemokine synthesis (Tran and Miller,2003;Huang et al.,2000).It is well established that triggering the innate immune response in the brain produces neuroinflammation (DeLeo et al.,2004).This is accompanied by influx into the brain of a variety of leukocytes that contribute to additional aspects of the response (Babcock et al.,2003;Eugenin et al.,2006).Several studies have demonstrated that chemokines synthesized by activated micro-glia and astrocytes are the major mediators of leukocyte migra-tion into the brain (Fife et al.,2000;Huang et al.,2001;Babcock et al.,2003;Eugenin et al.,2006).It is already apparent from studies on the development of the nervous system that chemokines can act as regulators of neural progenitor migration.Activation of CXC chemokine receptor CXCR 4expressed by embryonic neural progenitors has been shown to be important for the formation of several structures in the brain,including the cerebellum (Zou et al.,1998),dentate gyrus (Bagri et al.,2002;Lu et al.,2002),cortex (Stumm et al.,2003),and in the formation of the DRG (Bel-madani et al.,2005).The use of cultured brain slices to examine the migratory potential of progenitors in response to chemokines has also been used previously.It has been demonstrated by our laboratory,and also by Bagri et al.(2002),that stromal cell-derived factor-1(SDF-1)/CXCR 4signaling is important for the migration of embryonic progenitors from the wall of the lateral ventricle to the nascent dentate gyrus.Animals that lack CXCR 4receptors exhibit deficits in the formation of the DG attributable to incomplete migration of granule cell progenitors.Bagri et al.(2002)demonstrated the effect of SDF-1on progenitors by forc-ing the expression of SDF-1at ectopic sites in embryonic hip-pocampal slice cultures and observing the inappropriate migra-tion of progenitors to these sites.In the present case,injection of an inflammatory stimulus was predicted to activate microglia and astrocytes,which would act as a source of chemokines.The secreted chemokines presumably form some type of concentra-tion gradient within the slice.It should be noted that,in the extracellular environment,chemokines are highly susceptible to degradation by enzymes such as tissue metalloproteinases (Scot-ton et al.,2002;Zhang et al.,2003;Parks et al.,2004).Moreover,chemokines bind to elements of the extracellular matrix (ECM),a mechanism that has been suggested in aiding the establishment of chemokine gradients.Chemokines attached in this way are stabilized against degradation (Scotton et al.,2002).Thus,a com-bination of these various factors might set up chemokine gradi-ents within the slices.

In our studies,we used several types of stimuli to initiate a neuroinflammatory response in the slice.All of these have been demonstrated to produce neuroinflammation in vivo (Mehlhorn et al.,2000;Dawson et al.,2003;Giovannini et al.,2003;Ambro-sini et al.,2005).It is likely,however,that the release of TNF-?–IFN-?and other proinflammatory cytokines form a

common

Figure 7.Migration of NPs in response to different inflammatory stimuli.EGFP-labeled NPs from wild-type CD1mice were grafted into the area of the DG of 7-d-old hippocampal slice in control cultures (A ,A ?,A ?).After 3d,cells migrated toward the inflammatory site (red spot)of slices treated with TNF ?–IFN-?(B ),gp120–CD4(B ?),or LPS (B ?).After 72h of transplanta-tion,the extent of cell “migration”(ECM)was found in the range of 584?18?m in control slices.Itincreasedby54%inLPS-injectedslices(p ?0.001;n ?3and9slicespergroup),43%in TNF-?–IFN-?-injected slices (p ?0.01;n ?5and 9slices per group),and 39%in HIV-1–gp120-injected slices (p ?0.001;n ?3and 9slices per group).Confocal images in A ?,B ?,and C ?show PKH26-labeled NPs (red)grafted into the area of the DG of 7-d-old slice cultures injected with an EGFP–?-amyloid-expressing adenovirus [fimbria (fi),green cells in A ?].After 3d,cells migrated toward the inflammatory site (red spot in B ?,C ?).Scale bar,500?m.

Belmadani et al.?Chemokines and Neural Progenitor Migration J.Neurosci.,March 22,2006?26(12):3182–3191?3187

link between all of these stimuli.These cy-tokines then trigger many events that co-ordinate the downstream elements of neu-roinflammation,of which chemokine synthesis is clearly an important part.We found that deletion of signaling by the CCR 2chemokine receptor appeared to be particularly important in the migratory re-sponse to neuroinflammation.Several ob-servations support this possibility.Neural progenitors express CCR 2receptors,and MCPs act as chemoattractants for these cells (Ji et al.,2004;Tran et al.,2004a;Widera et al.,2004).Furthermore,neu-roinflammatory stimuli such as those used here strongly upregulate MCP-1synthesis (Fife et al.,2000;Mehlhorn et al.,2000;Thibeault et al.,2001;Dawson et al.,2003).MCP-1is known to be synthesized in vivo under neuroinflammatory circumstances,and it is important in the trafficking of leu-kocytes into the brain (Fife et al.,2000;Babcock et al.,2003;Cardona et al.,2003;Dawson et al.,2003;Eugenin et al.,2006).Thus,it seems reasonable that MCP-1might play a similar role in the migration of progenitor cells.Our observations do suggest that several other chemokines are synthesized in response to neuroinflam-matory stimuli in the slice in addition to MCP-1.For example,large quantities of IP-10,RANTES,and GRO-?are also made,and there are numerous other che-mokines that we did not measure.Because receptors for these chemokines are simi-larly expressed by neural progenitors (Ji et al.,2004;Krathwohl and Kaiser,2004b;Tran et al.,2004a),it is not completely clear why block of MCP-1/CCR 2signaling has such a major effect.However,a similar problem applies to the major effect of this chemokine when considering its role in at-tracting leukocytes into the brain,because other chemokines are also synthesized un-der the same circumstances (Babcock et

al.,2003;Eugenin et al.,2006).Clearly,therefore,the mere synthesis of a chemo-kine only partially explains these data.The experiments imply that other chemokines such as RANTES and IP-10are less effec-tive chemoattractants for neural progeni-tors,at least under these circumstances.The reasons for this are unclear,but there are several possibilities.For example,me-tabolism of SDF-1yields a molecule that may act as an SDF-1antagonist or other properties,and it is possible that similar

things may occur in the case of other chemokines (Loetscher et

al.,1998;Zhang et al.,2003).In addition,the expression of dif-ferent chemokine receptors by the same cell can result in the

formation of heterodimers that may enhance or reduce the effects

of specific agonists.(Percherancier et al.,2005).

What evidence exists that mechanisms such as those suggested

here actually operate in vivo ?Numerous papers have demon-strated the homing of neural progenitor cells to sites of brain injury.Increased neurogenesis and migration of progenitors has been noted in animal models of epilepsy,stroke,trauma,Alzhei-mer’s disease,Parkinson’s disease,and Huntington’s disease (Parent,2003).In demyelinating diseases such as EAE or in

the Figure 8.Activation of macrophages/microglia and migration of transplanted cells in response to inflammatory stimuli in

7-d-old hippocampal slice cultures.In A and B ,hippocampal slices were generated from CX3CR 1–EGFP transgenic mice,in which EGFP–CX3CR 1

is mostly expressed in microglia.When injected with the inflammatory stimulus (TNF-?–IFN-?)into the dentate

gyrus(dg),sliceshowsactivationofEGFP–CX3CR 1-expressingmicrogliaatthesiteoftheinflammatorystimulus(B )andincontrol slice after saline injection (A ).In A ?,B ?,and C ?,slices were injected with EGFP–?-amyloid-expressing adenovirus as an inflam-matory stimulus.A ?is magnified in B ?(scale bar,200?m)and C ?(scale bar,100?m),showing CD11b-and F4/80-stained microglia/macrophages at the injection site of the inflammatory stimulus (red in A ?,B ?,C ?).In A ?and B ?,grafted GFP–NPs in slices injected with TNF-?–IFN-?were immunostained for macrophage/microglia markers using a combined cyanine

3-conjugated monoclonal to CD11b ?and F4/80?

.Box in A ?is magnified in B ?(scale bar,200?m).Box in B ?is magnified in C ?,D ?,and E ?(scalebar,20?m),whichshowsCD11b/F4/80-stainedmacrophage/microglia(redin D ?,E ?).Imagesin C ?and D ?were mergedtoshowthatthereisnocolocalizationbetweenmigratingEGFP–NPsandactivatedmacrophage/microglia.n ?1,9slices

were used.

3188?J.Neurosci.,March 22,2006?26(12):3182–3191Belmadani et al.?Chemokines and Neural Progenitor Migration

shiverer mouse,progenitors migrate into the white matter in which they primarily form oligodendrocytes that remyelinate nerves,sometimes with clearly beneficial behavioral effects (Picard-Riera et al.,2002;Ben-Hur et al.,2003;Pluchino et al.,

2005).In some paradigms,these experiments were performed using transplanted progenitors such as those used here (Parent,2003;Imitola et al.,2004;Kelly et al.,2004;Lindvall et al.,2004).These cells clearly express diverse chemokine receptors (Tran et al.,2004a;Pluchino et al.,2005).In other studies,endogenous progenitors migrated toward damaged areas of the brain.For example,a recent study demonstrated that nestin–EGFP-expressing cells migrated toward glial tumors that had been transplanted previously into the brains of adult mice (Glass et al.,2005).Few studies have examined the question of whether pro-genitors normally express chemokine receptors in vivo .However,Lu et al.(2002)did demonstrate the expression of CXCR 4by progenitors in the SGZ of the adult mouse hippocampus.In ad-dition,we demonstrated that progenitors normally residing in the subgranular zone,SVZ,and olfactory bulb express a number of other chemokine receptors in addition to CXCR 4(Tran et al.,2004b).Interestingly,three recent studies have demonstrated that some progenitor cells on the periphery of brain lesions ex-press CXCR 4receptors and that interference with CXCR 4-mediated signaling can interfere with the migration of these cells (Imitola et al.,2004;Kao and Price,2004;Kelly et al.,2004;Krath-wohl and Kaiser,2004a).

As our data also demonstrate,migration of progenitor cells away from the presumably neurogenic environment of the DG compromises their survival.According to data in the literature,this may be attributable to the synthesis of IL-6at neuroinflam-matory sites (Vallieres et al.,2002).Indeed,as we show here the synthesis of IL-6is also strongly upregulated by the inflammatory stimuli in our experiments.Consequently,the ultimate effect of the neuroinflammatory milieu on the development of neural progenitors may be complex.Chemokines may act to attract pro-genitors to the appropriate place for repair purposes.However,on their arrival,the progenitors may also be influenced by IL-6and other factors that may decrease their ability to survive (Monje et al.,2002,2003).Hence,the net result of CCR 2-mediated signaling may even be the ultimate overall demise of progenitor cells.

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Figure 9.Migration of EGFP–NPs in response to inflammation depends on MCP-1/CCR 2signaling.In A ,B ,and C ,EGFP–CCR 2KO labeled NPs (B6?129strain)were transplanted into the area of the DG of 7-d-old hippocampal slice cultures from wild-type mice (B6?129strain).In A ?,B ?,and C ?,wild-type EGFP-labeled NPs were transplanted into the area of the DG of 7-d-old slice cultures from MCP-1KO mice.After 3d,cells failed to show oriented migration toward the inflammatory site (red spot)in TNF-?–IFN-?-treated slices (B ,B ?).The extent of cell “migration”(ECM)in cytokine-treated group was found in the same range as in control group.In A and B,the ECM was 612?40versus 562?31?m in control group.In A ?and B ?,the ECM was 875?45versus 824?87?m in control group.However,when wild-type EGFP-labeled NPs were transplanted into the area of the DG of wild-type slices (all from B6?129strain),the ECM increased by 34%over control values (p ?0.01;n ?2and 9slices per group).Scale bar,500?

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