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一株产油酵母菌摇瓶产油发酵条件的优化

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一株产油酵母菌摇瓶产油发酵条件的优化

作者:马勇陈秀莉刘晓光

来源:《湖北农业科学》2014年第01期

摘要:以产油酵母突变菌株Y1m为试验菌株,通过单因素试验对突变菌株Y1m发酵条件进行了优化。结果表明,该突变菌株最适发酵产脂条件为发酵时间5 d,100 mL摇瓶装液量20 mL,发酵液初始pH 6.0,种子液接种量为10%,温度27 ℃,产脂培养基中硫酸锌含量为0.5 mg/L,氯化钙含量为0.3 g/L,氯化铁含量为0.5 mg/L,硫酸铜含量为0.05 mg/L时,突变菌株Y1m在上述条件下油脂产量最高。

关键词:产油酵母;产脂发酵;金属离子;优化

中图分类号:TQ920.1 文献标识码:A 文章编号:0439-8114(2014)01-0025-03

Optimizing Fermentation Conditions for Oil Production from A Strain of Oleaginous Yeast with Shake Flask

MA Yong,CHEN Xiu-li,LIU Xiao-guang

(Department of Biological Sciences and Technology, Baotou Teachers College, Baotou 014030, Inner Mongolia, China)

Abstract: Using the oleaginous yeast mutant strain Y1m as material, conditions for its lipid fermentation were optimized with single factor experiment. The results showed that the optimum fermentation conditions were as follows: 5 d, 20 mL volume of 100 mL shake flask, 100% seed inoculation of liquid,pH 6.0, 27 ℃, lipid fermentation with the concentration of ZnSO4,

CaCl2, FeCl3, CuSO4 being 0.5 mg/L, 0.3 g/L, 0.5 mg/L, 0.05 mg/L, respectively.

Key words: oleaginous yeast; lipid fermentation; metal ion; optimization

收稿日期:2013-05-10

基金项目:包头师范学院青年科学研究基金项目(BSYKJ2011-17)

作者简介:马勇(1980-),男,辽宁台安人,实验师,在读博士研究生,主要从事微生物油脂及植物生理与农作物基因工程方面研究,(电话)

136********(电子信箱)besbandon@https://www.wendangku.net/doc/368885039.html,;通讯作者,刘晓光(1963-),男,内蒙古海拉尔人,教授,硕士生导师,主要从事动物

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