TGF-β1诱导胃癌细胞上皮间质转化及其上调CD44表达的实验研究

TGF-β1诱导胃癌细胞上皮间质转化及其上调CD44表达

的实验研究

蔡成;俞继卫;吴巨钢;陆瑞祺;倪晓春;王守练;姜波健

【期刊名称】《国际外科学杂志》

【年(卷),期】2012(039)011

【摘要】Objective To investigate the biological effect of epithelial-to-mesenchymal transition (EMT) under the treatment of transforming growth factor (TGF)-β1 on the human gastric cancer cell line SGC7901 in vitro,and to observe whether the TGF-β1 can generate the tumor initiating cells ability in SGC7901 or not.Methods SGC7901 cells were cultured with TGF-β1.The morphological change was observed.The effect on proliferation of SGC7901 cells was detected by CCK-8.The invasion assay was used to investigate the motility and the invasion ability of SGC7901 cells.Immuofluorescence was used to detect the expression of E-cadherin and N-cadherin.The mRNA and protein's expression levels of EMT-related factors and CD44 were analyzed by RT-PCR and Western blotting respectively.Results TGF-β1 induced morphological alterations from epithelial to mesenchymal cells.The proliferation of SGC7901 cells was inhibited,and the ability of motility and invasion of SGC7901 cells were greatly enhanced after being treated with TGF-β1.RT-PCR and Western blotting showed that the expression of Snail (P ＜0.05),N-cadherin (P ＜0.05) and CD44 (P ＜