pEASY-Blunt clonning vector 载体说明书WORD版
pEASY-Blunt
Complete sequence AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGA TTCATTAATGCAGCTGGCACGA CAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCA TTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGA T AACAATTTCACACAGGAAACAGCTATGACCATGA TTACGCCAAGCTTGGTACCGAGCTCGGATC CACTAGTAACGGCCGCCAGTGTGCTGGAATT GCCCTTAAGGGCAA TTCTGCAGA TATCCA TC ACACTGGCGGCCGCTCGAGCATGCA TCTAGAGGGCCCAA TTCGCCCTATAGTGAGTCGTA TTAC AA TTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATC GCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCC TTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCG GCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCT TTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAA TCGGGG GCTCCCTTTAGGGTTCCGA TTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT GA TGGTTCACGTAGTGGGCCATCGCCCTGA TAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCA CGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTA TCTCGGTCTATTCT TTTGATTTA TAAGGGA TTTTGCCGA TTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAA ATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAG AAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAA TGTCAGCTACTGGGCTA TCTGGACA AGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACA TGGCGA TAGCT AGACTGGGCGGTTTTA TGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAA GGTTGGGAAGCCCTGCAAAGTAAACTGGA TGGCTTTCTTGCCGCCAAGGA TCTGATGGCGCAG GGGATCAAGATCTGA TCAAGAGACAGGA TGAGGATCGTTTCGCATGATTGAACAAGA TGGATTG CACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACA ATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCA AGACCGACCTGTCCGGTGCCCTGAA TGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGG CCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGC TGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCA TCCCACCTTGCTCCTGCCGAGAAAGT ATCCATCATGGCTGA TGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACC ACCAAGCGAAACATCGCA TCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGG ATGA TCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGC GCATGCCCGACGGCGAGGA TCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAA TATCA TGGT GGAAAATGGCCGCTTTTCTGGA TTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAG GACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCC TCGTGCTTTACGGTATCGCCGCTCCCGA TTCGCAGCGCA TCGCCTTCTA TCGCCTTCTTGACGAG TTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTG AAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGA TCCTTG AGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGC GGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCA TCTTACGGATGGCA TGACAGTAAGAGAA TTATGCAGTGCTGCCA TAACCATGAGTGA TAACACTGCGGCCAACTTACTTCTGACAACGA TCG GAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGA TCG TTGGGAACCGGAGCTGAA TGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGC AA TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTG
GCTGGTTTATTGCTGA TAAA TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT GGGGCCAGATGGTAAGCCCTCCCGTA TCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG GA TGAACGAAATAGACAGA TCGCTGAGA TAGGTGCCTCACTGATTAAGCA TTGGTAACTGTCAG ACCAAGTTTACTCATA TATACTTTAGATTGA TTTAAAACTTCATTTTTAATTTAAAAGGATCTAGG TGAAGA TCCTTTTTGA TAA TCTCA TGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCG TCAGACCCCGTAGAAAAGA TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTG CTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAAC TCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAG CCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACA TACCTCGCTCTGCTAATCCT GTTACCAGTGGCTGCTGCCAGTGGCGA TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAG TTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGA GCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACG AGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGAC TTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTA TGGAAAAACGCCAGCAACG CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCC CTGA TTCTGTGGATAACCGTA TTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAAC GACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG
Lac Z fragment: bases 1-548
M13 reverse priming site: bases 205-221
Multiple cloning site: bases 234-358
T7 promoter/priming site: bases 364-381
M13 Forward (-20) priming site: bases 389-404
f1 origin: bases 546-983
Kanamycin resistance ORF: bases 1317-2111
Ampicillin resistance ORF: bases 2129-2989
pUC origin: bases 3134-3807
pEASY-Blunt
Restriction Map
Enzyme # of cuts positions
Acc65I (1) ggtacc 246
AceIII (3) cagctc 949, 2630, 3870
AciI (47) ccgc 88, 191, 329, 561, 575,
1014, 1159, 1515,
1579, 1680
AclI (2) aacgtt 2319, 2692
AcuI (4) ctgaag 3283, 1576, 2008,
2235
AflII (1) cttaag 297
AflIII (2) acrygt 1021, 3810
AhdI (1) gacnnnnngtc 2922
AloI (1) gaacnnnnnntcc 835
AluI (22) agct 58, 122, 217, 239, 251,
477, 691, 948, 1069,
1122
AlwI (16) ggatc 258, 1700, 2292, 3072,
3169, 257, 1263, 1280,
1310, 1621
AlwNI (2) cagnnnctg 1073, 3399
ApaBI (1) gcannnnntgc 1002
ApaI (1) gggccc 356
ApaLI (2) gtgcac 2250, 3496
ApoI (3) raatty 964, 975, 986
AseI (3) attaat 52, 111, 2746
AvaI (1) cycgrg 338
AvaII (3) ggwcc 1966, 2557, 2779
BamHI (1) ggatcc 258
BanI (7) ggyrcc 137, 246, 740, 1202,
1450, 1485, 2969 BanII (4) grgcyc 252, 356, 706, 1812
BbeI (2) ggcgcc 1202, 1450
BbvI (18) gcagc 1002, 1410, 1594,
1687, 2500, 3194,
3400, 57, 457, 530 BccI (11) ccatc 771, 1243, 1269, 1334,
1762, 2470, 2757,
2881, 319, 789 BceAI (6) acggc 397, 1424, 3324, 270,
737, 1860
BcefI (6) acggc 397, 1424, 3324, 270,
737, 1860
BcgI (1) cgannnnnntgc 2401
BciVI (2) gtatcc 1659, 3601
BclI (1) tgatca 1292
BfaI (7) ctag 263, 349, 626, 1150,
2727, 3062, 3315 BfrBI (1) atgcat 345
BglI (2) gccnnnnnggc 539, 2803
BglII (1) agatct 1287
Bme1580I (5) gkgcmc 356, 1393, 1486, 2250,
3496
BmrI (5) actggg 420, 1075, 1156, 1390,
2872
BpmI (1) ctggag 2832
BpuEI (5) cttgag 1842, 3442, 2297,
3165, 3704
BsaAI (3) yacgtr 779, 1022, 1753 BsaBI (3) gatnnnnatc 319, 1286, 1310 BsaHI (3) grcgyc 1202, 1450, 2381 BsaI (1) ggtctc 2850
BsaJI (7) ccnngg 142, 430, 1056, 1260,
1614, 1883, 3650 BsaWI (5) wccggw 1184, 1482, 2626,
3457, 3604
BsaXI (2) acnnnnnctcc 833, 28
BscAI (11) gcatc 1418, 1673, 1889,
2230, 2670, 3722, 346,
1740, 1804, 2079 BseMII (4) ctcag 2960, 3126, 3535,
2420
BseYI (3) cccagc 1197, 1955, 3506 BsiEI (8) cgrycg 272, 332, 508, 1357,
2401, 2550, 3473,
3897
BsiHKAI (6) gwgcwc 252, 1560, 1750, 2250,
2335, 3496
BslI (9) ccnnnnnnngg 39, 561, 887, 1431,
1975, 3331, 3610,
3776, 3794
BsmAI (2) gtctc 1298, 2850
BsmFI (1) gggac 1587
Bsp1286I (11) gdgchc 252, 356, 706, 1393,
1486, 1560, 1750,
1812, 2250, 2335
Bsp24I (1) gacnnnnnntgg 3130
BspCNI (4) ctcag 2960, 3126, 3535,
2420
BspHI (1) tcatga 3090
BspMI (3) acctgc 1118, 1347, 1710 BsrBI (5) ccgctc 191, 335, 635, 2062,
3879
BsrDI (3) gcaatg 2863, 1677, 2681 BsrFI (4) rccggy 676, 1770, 1951, 2837 BsrI (20) actgg 277, 1032, 2446, 3394,
3407, 81, 326, 393,
419, 864
BssHII (1) gcgcgc 1848
BssSI (3) cacgag 2043, 2253, 3637 BstAPI (1) gcannnnntgc 1002
BstF5I (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937 BstKTI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780 BstNI (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
BstUI (12) cgcg 33, 550, 574, 594, 970,
1514, 1815, 1847,
2360, 2853
BstXI (2) ccannnnnntgg 284, 326
BstYI (11) rgatcy 258, 1264, 1287, 1622,
1868, 2275, 2292,
3060, 3072, 3158 BtgI (1) ccrygg 1883
BthCI (34) gcngc 57, 273, 330, 457, 530,
562, 576, 598, 1002,
1255
BtsI (4) gcagtg 2524, 94, 1129, 2496 Cac8I (24) gcnngc 61, 90, 343, 476, 500,
619, 633, 676, 1101,
1125
CdiI (7) catcg 1888, 2669, 790, 1736,
1939, 2080, 2265 ChaI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780 CjeI (7) ccannnnnngt 3228, 3783, 243, 482,
3129, 3229, 3307 CjePI (4) ccannnnnnntc 3277, 2538, 3010,
3130
Csp6I (3) gtac 245, 1754, 2438 CviAII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510 CviJI (78) rgcy 41, 58, 122, 144, 165,
217, 239, 251, 271,
331
DdeI (4) ctnag 2420, 2960, 3126,
3535
DpnI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780 DraI (3) tttaaa 2342, 3034, 3053 DraIII (1) cacnnngtg 782
DrdI (3) gacnnnnnngtc 828, 1478, 3708
EaeI (9) yggccr 42, 272, 332, 396,
1357, 1531, 1922,
1949, 2529
EagI (3) cggccg 272, 332, 1357
EarI (4) ctcttc 494, 1800, 2010, 2128 EciI (4) ggcgga 1964, 2764, 3592,
3738
Eco57MI (5) ctgrag 3283, 1576, 2008,
2235, 2832
EcoHI (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431 EcoICRI (1) gagctc 252
EcoO109I (1) rggnccy 356
EcoRV (1) gatatc 315
EsaBC3I (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
FalI (1) aagnnnnnctt 1773
FatI (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510 FauI (6) cccgc 89, 579, 1580, 32, 500,
633
FmuI (8) ggncc 355, 498, 786, 1966,
2557, 2779, 2796,
2875
Fnu4HI (34) gcngc 57, 273, 330, 457, 530,
562, 576, 598, 1002,
1255
FokI (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937 FspI (3) tgcgca 528, 1551, 2697
GdiII (11) cggccr 42, 272, 332, 396,
1357, 1922, 1949, 272,
332, 1357
HaeI (4) wggccw 1531, 3334, 3786,
3797
HaeII (6) rgcgcy 7, 622, 630, 1202,
1450, 3566
HaeIII (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
HaeIV (2) gaynnnnnrtc 1628, 2922
HgaI (4) gacgc 3130, 3708, 549, 2380 HhaI (30) gcgc 6, 34, 99, 527, 551,
564, 573, 595, 621,
629
Hin4I (8) gaynnnnnvtc 257, 346, 1628, 1658,
2848, 2922, 1628,
2922
HindIII (1) aagctt 240
HinfI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910 HinP1I (30) gcgc 6, 34, 99, 527, 551,
564, 573, 595, 621,
629
HpaII (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702 HphI (6) ggtga 2202, 2443, 768, 2217,
2839, 3066
Hpy188I (15) tcnga 255, 721, 1266, 1289,
1415, 1759, 2112,
2421, 2541, 2552 Hpy188III (21) tcnnga 77, 350, 415, 1084,
1284, 1295, 1468,
1784, 1793, 1876 Hpy8I (7) gtnnac 776, 831, 848, 1236,
2250, 3008, 3496 Hpy99I (9) cgwcg 399, 412, 1536, 1564,
1858, 1873, 2652,
2915, 3709
HpyAV (10) ccttc 1584, 2562, 3270,
3580, 514, 654, 2085,
2095, 2187, 2799 HpyCH4I (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510 HpyCH4III (11) acngt 522, 804, 1045, 1828,
1944, 2052, 2479,
2994, 3307, 3777 HpyCH4IV (11) acgt 410, 668, 778, 821,
833, 1021, 1565, 1752,
2318, 2691
HpyCH4V (17) tgca 55, 309, 344, 455,
1126, 1228, 1340,
1499, 1674, 1688 HpyF10VI (25) gcnnnnnnngc 15, 99, 143, 290, 339,
363, 509, 539, 571,
615
KasI (2) ggcgcc 1202, 1450
KpnI (1) ggtacc 246
LpnI (6) rgcgcy 7, 622, 630, 1202,
1450, 3566
M.AacDam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.AclI (2) aacgtt 2319, 2692
M.AflIII (2) acrygt 1021, 3810
M.AlaK2I (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.AloI (1) gaacnnnnnntcc 835
M.AluI (22) agct 58, 122, 217, 239, 251,
477, 691, 948, 1069,
1122
M.Alw26I (2) gtctc 1298, 2850
M.AlwI (16) ggatc 258, 1700, 2292, 3072,
3169, 257, 1263, 1280,
1310, 1621
M.ApaI (1) gggccc 356
M.AquI (1) cycgrg 338
M.AvaIX (4) rccggy 676, 1770, 1951, 2837 M.AvaV (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.AvaVI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.AvaVII (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.BabI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.BanII (4) grgcyc 252, 356, 706, 1812 M.BbvI (18) gcagc 1002, 1410, 1594,
1687, 2500, 3194,
3400, 57, 457, 530 M.BbvSI (18) gcwgc 57, 457, 530, 598,
1002, 1410, 1511,
1553, 1594, 1687
M.BcgI (1) cgannnnnntgc 2401
M.BclI (1) tgatca 1292
M.BcnIA (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431
M.BcnIB (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431
M.BepI (12) cgcg 33, 550, 574, 594, 970,
1514, 1815, 1847,
2360, 2853
M.BfiIA (5) actggg 420, 1075, 1156, 1390,
2872
M.BfiIB (5) actggg 420, 1075, 1156, 1390,
2872
M.BflBF4I (18) gcsgc 273, 330, 562, 576,
1255, 1358, 1421,
1516, 1681, 1923
M.BglII (1) agatct 1287
M.BmeTI (1) tgatca 1292
M.BnaI (1) ggatcc 258
M.BsaAI (3) yacgtr 779, 1022, 1753
M.BspKT6I (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.BspLU11IIIA (1) gggac 1587
M.BspLU11IIIC (1) gggac 1587
M.BspRI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.BspST5I (11) gcatc 1418, 1673, 1889,
2230, 2670, 3722, 346,
1740, 1804, 2079
M.BstF5IA (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937
M.BstF5ID (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937
M.BstI (1) ggatcc 258
M.BstNI (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
M.BstVI (1) ctcgag 338
M.BstYI (11) rgatcy 258, 1264, 1287, 1622,
1868, 2275, 2292,
3060, 3072, 3158
M.BsuBI (2) ctgcag 310, 1500
M.BsuEII (12) cgcg 33, 550, 574, 594, 970,
1514, 1815, 1847,
2360, 2853
M.BsuFI (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
M.BsuRI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.CboI (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
https://www.wendangku.net/doc/3c18379361.html,rMI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.Cfr10I (4) rccggy 676, 1770, 1951, 2837 M.Cfr13I (8) ggncc 355, 498, 786, 1966,
2557, 2779, 2796,
2875
M.Cfr6I (4) cagctg 59, 478, 1195, 1555 M.CfrBI (2) ccwwgg 1260, 1883
M.CfrI (9) yggccr 42, 272, 332, 396,
1357, 1531, 1922,
1949, 2529
M.CglASI (18) gcsgc 273, 330, 562, 576,
1255, 1358, 1421,
1516, 1681, 1923
M.CviAI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.CviAII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.CviAIV (260) rgcb 7, 23, 41, 58, 122, 144,
165, 217, 234, 239 M.CviBI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.CviBIII (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.CviJI (260) rgcb 7, 23, 41, 58, 122, 144,
165, 217, 234, 239 M.CviPI (309) gc 4, 15, 22, 32, 40, 54,
57, 61, 86, 90
M.CviQI (3) gtac 245, 1754, 2438
M.CviQIII (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.CviQVI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.CviQVII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.CviRI (17) tgca 55, 309, 344, 455,
1126, 1228, 1340,
1499, 1674, 1688
M.CviRII (3) gtac 245, 1754, 2438
M.CviSI (17) tgca 55, 309, 344, 455,
1126, 1228, 1340,
1499, 1674, 1688
M.CviSII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.CviSIII (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.CviTI (78) rgcy 41, 58, 122, 144, 165,
217, 239, 251, 271,
331
M.DdeI (4) ctnag 2420, 2960, 3126,
3535
M.DpnIIA (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
1621, 1699, 1780
M.DraI (3) tttaaa 2342, 3034, 3053
M.DraIII (1) cacnnngtg 782
M.DsaV (12) ccngg 142, 430, 1056, 1453,
1613, 1836, 2384,
2735, 3431, 3649
M.EaeI (9) yggccr 42, 272, 332, 396,
1357, 1531, 1922,
1949, 2529
M.Ecl18kI (12) ccngg 142, 430, 1056, 1453,
1613, 1836, 2384,
2735, 3431, 3649
M.Eco1831I (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431
M.Eco57IA (4) ctgaag 3283, 1576, 2008,
2235
M.Eco57IB (4) ctgaag 3283, 1576, 2008,
2235
M.Eco67Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.EcoHI (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431
M.EcoHK31I (9) yggccr 42, 272, 332, 396,
1357, 1531, 1922,
1949, 2529
M.EcoKDam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.EcoKDcm (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
M.EcoKI (1) aacnnnnnngtgc 2259
M.EcoO109I (1) rggnccy 356
M.EcoP15I (3) cagcag 3193, 3402, 3286
M.EcoP1Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.EcoPI (6) agacc 890, 1772, 2849, 1470,
3000, 3135
M.EcoR124II (1) gaannnnnnnrtcg 1741
M.EcoRII (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
M.EcoRV (1) gatatc 315
1621, 1699, 1780
M.EcoT2Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.EcoT4Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.EsaBC1I (22) agct 58, 122, 217, 239, 251,
477, 691, 948, 1069,
1122
M.EsaBC3I (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.EsaBS1I (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.EsaDix3I (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.EsaDix4I (18) ttaa 51, 110, 296, 445, 569,
840, 938, 955, 966,
978
M.EsaDix5I (18) ttaa 51, 110, 296, 445, 569,
840, 938, 955, 966,
978
M.EsaLHCI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.FnuDII (12) cgcg 33, 550, 574, 594, 970,
1514, 1815, 1847,
2360, 2853
M.FokI (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937
M.HaeIII (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.HaeIV (2) gaynnnnnrtc 1628, 2922
M.HapII (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
M.HgaIA (4) gacgc 3130, 3708, 549, 2380 M.HgaIB (4) gacgc 3130, 3708, 549, 2380
M.HgiCI (7) ggyrcc 137, 246, 740, 1202,
1450, 1485, 2969
M.HhaI (30) gcgc 6, 34, 99, 527, 551,
564, 573, 595, 621,
629
M.HhaII (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.HindDam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.HindI (99) cac 94, 114, 176, 186, 278,
372, 414, 581, 586,
601
M.HindIII (1) aagctt 240
M.HinfI (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.HinHP1Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.HinP1I (30) gcgc 6, 34, 99, 527, 551,
564, 573, 595, 621,
629
M.HpaII (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
M.HphIA (6) ggtga 2202, 2443, 768, 2217,
2839, 3066
M.HphIB (6) ggtga 2202, 2443, 768, 2217,
2839, 3066
M.Hpy166IV (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.Hpy188I (15) tcnga 255, 721, 1266, 1289,
1415, 1759, 2112,
2421, 2541, 2552
M.Hpy188II (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.Hpy8I (7) gtnnac 776, 831, 848, 1236,
2250, 3008, 3496
M.Hpy99II (6) gtsac 416, 603, 1571, 1877,
2449, 2660
M.Hpy99IX (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.Hpy99VA (18) cctc 352, 495, 1307, 1371,
1507, 1864, 2554,
2760, 3638, 3921
M.Hpy99VI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.Hpy99X (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.Hpy99XI (11) acgt 410, 668, 778, 821,
833, 1021, 1565, 1752,
2318, 2691
M.HpyAI (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.HpyAIIA (11) gaaga 649, 2109, 3160, 3312,
493, 1799, 2009, 2127,
2236, 2314
M.HpyAIII (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.HpyAIV (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.HpyAIX (7) gtnnac 776, 831, 848, 1236,
2250, 3008, 3496
M.HpyAVIII (30) gcgc 6, 34, 99, 527, 551,
564, 573, 595, 621,
629
M.HpyAX (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.HpyCH4IV (11) acgt 410, 668, 778, 821,
833, 1021, 1565, 1752,
2318, 2691
M.HpyI (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.KpnI (1) ggtacc 246
M.LlaDCHIA (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.LlaDCHIB (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.LlaKR2I (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.MboIA (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.MboIB (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.MboIIA (11) gaaga 649, 2109, 3160, 3312,
493, 1799, 2009, 2127,
2236, 2314
M.MjaVI (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
M.MlyI (6) gastc 376, 828, 850, 2922,
3439, 3910
M.MmeIA (3) tccrac 825, 3436, 3620
M.MmeIB (3) tccrac 825, 3436, 3620
M.MnlIA (18) cctc 352, 495, 1307, 1371,
1507, 1864, 2554,
2760, 3638, 3921
M.MnlIB (18) cctc 352, 495, 1307, 1371,
1507, 1864, 2554,
2760, 3638, 3921
M.MseI (18) ttaa 51, 110, 296, 445, 569,
840, 938, 955, 966,
978
M.MspI (21) ccgg 163, 675, 1056, 1183,
1354, 1431, 1453,
1481, 1612, 1702
M.MthTI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.MthZI (7) ctag 263, 349, 626, 1150,
2727, 3062, 3315
M.MvaI (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
M.NgoBIX (3) gtannnnnctc 2261, 3543, 252
M.NgoBVIIIA (6) ggtga 2202, 2443, 768, 2217,
2839, 3066
M.NgoGII (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.NgoHVIII (6) ggtga 2202, 2443, 768, 2217,
2839, 3066
M.NgoMIV (2) gccggc 676, 1951
M.NgoMV (16) ggnncc 137, 246, 258, 356,
707, 719, 740, 1202,
1450, 1485
M.NgoMXV (59) gcchr 40, 61, 90, 134, 143,
327, 394, 431, 480,
785
M.NgoPII (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.NlaIII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510
M.NlaX (12) ccngg 142, 430, 1056, 1453,
1613, 1836, 2384,
2735, 3431, 3649
M.NmeSI (1) agtact 2439
M.NspHI (3) rcatgy 343, 1852, 3810
M.NspI (3) rcatgy 343, 1852, 3810
M.PaeR7I (1) ctcgag 338
M.PgiI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.Phi3TI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.Phi3TII (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.PhiCh1I (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.PhiMx8I (4) ctssag 310, 338, 1500, 2832 M.PspPI (8) ggncc 355, 498, 786, 1966,
2557, 2779, 2796,
2875
M.PstI (2) ctgcag 310, 1500
M.PvuII (4) cagctg 59, 478, 1195, 1555 M.Rho11sI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.RsaI (3) gtac 245, 1754, 2438
M.SacI (1) gagctc 252
M.Sau3239I (1) ctcgag 338
M.Sau3AI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.Sau96I (8) ggncc 355, 498, 786, 1966,
2557, 2779, 2796,
2875
M.SauLPI (2) gccggc 676, 1951
M.ScaI (1) agtact 2439
M.SenPI (12) ccngg 142, 430, 1056, 1453,
1613, 1836, 2384,
2735, 3431, 3649
M.SinI (3) ggwcc 1966, 2557, 2779
M.SmaII (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.SPBetaI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.SPRI (19) ggcc 41, 271, 331, 355, 395,
497, 786, 928, 1356,
1530
M.SscL1I (10) gantc 46, 376, 828, 850,
1935, 2069, 2922,
3439, 3835, 3910
M.Sse9I (15) aatt 108, 183, 200, 288,
304, 360, 387, 963,
974, 985
M.SsoII (12) ccngg 142, 430, 1056, 1453,
1613, 1836, 2384,
2735, 3431, 3649
M.SssI (263) cg 5, 14, 21, 31, 35, 42,
64, 76, 87, 98
M.Sth368I (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.StsI (10) ggatg 463, 1632, 1059, 1242,
1304, 1761, 1786,
2469, 2756, 2937
M.Sty1344Dam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.StyDam (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.StyLTI (6) cagag 1207, 1414, 3351,
3377, 3697, 3288
M.StyLTIII (1) gagnnnnnnrtayg 2418
M.StySPI (1) aacnnnnnngtrc 2259
M.TaeII (1) tgatca 1292
M.TaqI (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.TfiI (4) gawtc 46, 1935, 2069, 3835 M.TpaI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780
M.Tsp45I (6) gtsac 416, 603, 1571, 1877,
2449, 2660
M.TspRI (14) castg 94, 278, 325, 392,
1129, 1573, 2496,
2523, 2870, 2975
M.Tth111I (1) gacnnngtc 1569
M.TthHB8I (9) tcga 337, 744, 1562, 1718,
1742, 1778, 1940,
2266, 3710
M.VspI (3) attaat 52, 111, 2746
M.XbaI (1) tctaga 350
M.XcmI (1) ccannnnnnnnntgg 1269
M.XhoI (1) ctcgag 338
M.XhoII (11) rgatcy 258, 1264, 1287, 1622,
1868, 2275, 2292,
3060, 3072, 3158
M.XmaIII (3) cggccg 272, 332, 1357 MaeIII (15) gtnac 267, 416, 436, 591,
603, 1571, 1877, 2261,
2449, 2602
MboI (25) gatc 257, 507, 1263, 1280,
1286, 1291, 1310,
1621, 1699, 1780 MboII (11) gaaga 649, 2109, 3160, 3312,
493, 1799, 2009, 2127,
2236, 2314
MlyI (6) gagtc 850, 3439, 376, 828,
2922, 3910
MmeI (3) tccrac 825, 3436, 3620
MnlI (18) cctc 352, 495, 1307, 1371,
1507, 1864, 2554,
2760, 3638, 3921
MscI (1) tggcca 1531
MseI (18) ttaa 51, 110, 296, 445, 569,
840, 938, 955, 966,
978
MslI (4) caynnnnrtg 1888, 2151, 2510,
2669
MspA1I (7) cmgckg 59, 478, 1195, 1555,
2284, 3225, 3470 MwoI (25) gcnnnnnnngc 15, 99, 143, 290, 339,
363, 509, 539, 571,
615
NaeI (2) gccggc 676, 1951
NarI (2) ggcgcc 1202, 1450
NciI (6) ccsgg 1056, 1453, 1613,
2384, 2735, 3431 NcoI (1) ccatgg 1883
NgoMIV (2) gccggc 676, 1951
NlaIII (14) catg 226, 342, 1139, 1320,
1665, 1851, 1882,
1908, 2474, 2510 NlaIV (16) ggnncc 137, 246, 258, 356,
707, 719, 740, 1202,
1450, 1485
Nli3877I (1) cycgrg 338
NotI (1) gcggccgc 333
NsiI (1) atgcat 345
NspI (3) rcatgy 343, 1852, 3810
PciI (1) acatgt 3810
PleI (6) gagtc 850, 3439, 376, 828,
2922, 3910
PpiI (3) gaacnnnnnctc 835, 3119, 2276
Ppu10I (1) atgcat 345
PsiI (1) ttataa 907
Psp03I (3) ggwcc 1966, 2557, 2779 PspGI (6) ccwgg 142, 430, 1836, 3649,
3662, 3783
PspOMI (1) gggccc 356
PssI (1) rggnccy 356
PstI (2) ctgcag 310, 1500
PvuI (2) cgatcg 508, 2550
PvuII (4) cagctg 59, 478, 1195, 1555 RsaI (3) gtac 245, 1754, 2438
RsrII (1) cggwccg 1967
SacI (1) gagctc 252
SapI (2) gctcttc 1801, 2011