Effects of pioglitazone on expressions of matrix metalloproteinases 2 and 9 in kidneys of

Effects of pioglitazone on expressions of matrix metalloproteinases 2 and 9 in kidneys of diabetic

rats

董凤芹;李红;蔡卫民;陶君;李群;阮昱;郑芬萍;张哲

【期刊名称】《中华医学杂志（英文版）》

【年(卷),期】2004(000)007

【摘要】Background The changes in matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were examined in the kidneys of diabetic rats to investigate the degradative pathway of collagen type Ⅳ (C-Ⅳ) and the protective effects of pioglitazone on an experimental model of diabetic nephropathy.Methods In 54 SD rats used in our study, 18 served as normal controls. Diabetes mellitus was induced in 36 age- and weight-matched rats by intraperitoneal injection of streptozotocin (70 mg/kg); 18 of the diabetic rats were allocated at random to receive pioglitazone (20 mg*kg-1*d-1) in their drinking water and 18 served as diabetic controls. Rats were killed after 2, 4, or 8 weeks of treatment. Kidneys were examined pathomorphologically and the expressions of MMP-2, MMP-9, and C-Ⅳ were analyzed by immunohistochemistry, and the results were quantified by image analysis techniques.Results Diabetes mellitus was associated with a decrease in the expression of MMP-2 in the glomeruli (P<0.05, vs control). By contrast, MMP-2 expression in the interstitium increased,