多发性骨髓瘤患者原代细胞与U266细胞株zeste基因增强子同源物2表达

多发性骨髓瘤患者原代细胞与U266细胞株zeste基因增强子同源物2表达升高的初步研究

马艳萍;贾谷;张灵

【期刊名称】《白血病·淋巴瘤》

【年(卷),期】2013(022)005

【摘要】Objective To investigate the expression of DNA methylation related protein enhancer of zeste homolog 2 (EZH2) in multiple myeloma (MM) cell line U266 and enriched MM cells (MM-BMMNC) from 10 patients,and analyze the potential role of DNA methylation in the occurrence and development of MM.Methods Human MM cell line U266 and MM cells from 10 MM patients were studied.Enriched bone marrow mononuclear cells (N-BMMNC) from 10 healthy persons were used as the control.The expression of EZH2 were determined by Western blot using the ratio of target protein EZH2 and the internal reference β-actin expression as the final results.Results The preliminary in vitro results showed that EZH2 expression was higher in MM cell line U266 and patient MM-BMMNC (N-BMMNC 0.2277±0.1306,MM-BMMNC 0.9220±0.1301,U266 0.9730±0.1029),with statistically significant differences (P ＜0.05).Conclusion EZH2 is enriched in U266 and MM-BMMNC.DNA methylation may have a close correlation with the occurrence and development of MM.%目的探讨多发性骨髓瘤(MM) U266细胞株和MM患者的骨髓单个核细胞(BMMNC)中DNA甲基化相关蛋