CFI-400945_fumarate_LCMS_24740_MedChemExpress

File ..70526 2017-05-26 14-00-39\FSD4294-006-2_1.D Tgt Mass (CHM): Injection Date : 26 May 17 3:13 pm +0800 Seq. Line : 17 Sample Name : FSD4294-006-2_1 Location : P1-A-07 Acq. Operator : CLJ_701 Inj : 1 Spec. Reported : MS Integration Inj Volume : 0.4 ul

Acq. Method : D:\chem32\1\DATA\20170526\20170526 2017-05-26 14-00-39\1-POS-3MIN.M Analysis Method : D:\CHEM32\1\DATA\20170526\20170526 2017-05-26 14-00-39\1-POS-3MIN.M Catalog No : HY-12300B Batch#24740 A-RP-186

Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min

Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C

min*

0.5

1

1.52

2.53mAU 0

5001000*DAD1 B, Sig=214,4 Ref=off

1.449

1.4891.555

1.651

1.722

1.828

min*

0.51

1.5

2 2.53mAU 0

200400

600*DAD1 C, Sig=254,4 Ref=off 1.452

1.489

1.5601.5921.6221.6511.724

1.829

min

0.51

1.5

2

2.5

3

10000

2000030000 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 70

1.711

min

0.5

1

1.5

2

2.5

3

-0.1

-0.0500.05 Ion 1, MSD1 1,Target Mass 0 +H Positive, EIC=0.7:1.7 Integration Results for DAD1 B, Sig=214,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.45 0.02 1.50 1.36 0.07 ND 1.49 0.02 2058.03 1644.59 98.38 ND 1.55 0.02 17.34 12.21 0.83 ND 1.65 0.02 3.62 3.80 0.17 268 1.72 0.02 8.08 5.48 0.39 268 1.83 0.02 3.37 3.45 0.16 268

Integration Results for DAD1 C, Sig=254,4 Ref=off

RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.45 0.03 1.79 1.51 0.16 ND 1.49 0.02 1081.71 868.08 98.57 ND 1.56 0.03 5.04 3.63 0.46 ND 1.59 0.03 0.70 0.71 0.06 268 1.62 0.07 0.26 0.31 0.02 268 1.65 0.04 1.19 1.25 0.11 268 1.72 0.03 5.10 3.12 0.47 268 1.83 0.04 1.57 1.28 0.14 268

MS Report from Instrument: LCMS-02

Integration Results for MSD1 TIC, MS File

RetTim Width Area Height Area% MS(+) |-------|--------|--------------|--------------|--------|------| 1.71 0.17 392351.09 37719.88 100.00 268

Ret. Time: 1.71 <<<< POSITIVE SPECTRA >>>>

m/z

200

300

400

500

600

700

800

2000

40006000

8000100001200014000 ES-API Positive

535.3

268.2