Herbicidal activity of Aureobasidium pullulans PA-2 on weeds and optimization of its solid

Herbicidal activity of Aureobasidium pullulans PA-

2 on weeds and optimization of its solid-state

fermentation conditions

GUO Qing-yun1, 2, 3＊,CHENG Liang1, 2, 3＊,ZHU Hai-xia1, 2, 3,Ll Wei1, 2, 3,WEl You-hai1, 2, 3,CHEN Hong-yu1, 2, 3,GUO Liang-zhi1, 2, 3,WENG Hua1, 2, 3,WANG Jian1, 4

【摘要】Abstract Fungal strain PA-2 was isolated from infected poplar leaves from the Ping’an District,Haidong City of Qinghai Province,China.Based on the culture characteristics and the internal transcribed spacer (ITS) sequence of its 16S rDNA,the strain was identified as Aureobasidium pullulans.The culture and metabolites of strain PA-2 showed high herbicidal potential to five tested weeds Galium aparine var. tenerum,Chenopodium album,Malva crispa, Polygonum lapathifolium and Avena fatua. For the in vitro test,5 days after the detached leaves were inoculated with PA-2 culture,all leaves infected by the hyphae and became black and rotten.For the in vivo test,a metabolite filtrate of PA-2 culture was sprayed over the living weed plants,and five days after inoculation,the weed plants became withered and necrotic.Seven days after inoculation,the fresh weight reductions of G.aparine var.tenerum,C.album,M.crispa,https://www.wendangku.net/doc/692466434.html,pathifolium and A.fatua were 87.25,78.46,82.25,62.11,and 80.27%,respectively.Galium aparine var.tenerum and M.crispa exhibited