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组胚切片报告

组织学与胚胎学 实验报告

指定主题:

实验一 、上皮组织 基本组织1

报告主题:假复层纤毛柱状上皮 主题属性:指定 标本号:27# 染色:HE 染色

教学要求:掌握假复层纤毛柱状上皮得光镜结构特征

光镜下可见假复层纤毛柱状上皮得各种上皮细胞界限不清,细胞核染蓝紫色,高矮不一,其中柱状细胞最多,游离面有大量纤毛,柱状细胞之前常夹有杯状细胞,其底部狭窄,顶部膨大,细胞核深染。所有细胞得基底膜均附着在基膜上,即使细胞核高低不齐排列,形似复

杯状细胞 Goblet cell

结缔组织 Connective Tissue

纤毛 Cilium

层,实为单层。Then visible pseudostratified ciliated columnar epithelium of epithelial cell line is not clear, the nucleus dyed purple blue, height is differ, the columnar cells most, free surface has a lot of ciliated columnar cells, before often clip has goblet cell, its bottom is narrow, the top swollen, with hyperchromatic nuclei 、 All the cells of the basement membrane are attached on the basement membrane, whose nucleus are irregular, and the shape see as pound layer, and it is a single 、实验二 结缔组织 基本组织2

报告主题:骨单位 主题属性:指定 标本号:6# 材料:骨切片

染色:Schmorl 氏法块染

教学要求:掌握骨组织得结构

图2骨单位(骨切片,Schmorl 氏法块染,40×100)

Figure 2 osteon (osseous smear,Schmorl stained,40×100)骨单位位于内外环骨板之间,数量最多,中央为圆心得中央管,中央管与穿通管相通,数层同心圆排列得骨板围绕中央管。在骨单位之间,有一些不规则排列得间骨板,其中无血管通道,在骨板之间或骨板中可见黄色小腔,即骨陷窝。Osteon is located in the inside and outside ring bone plate between, the largest

中央管 Central canal

骨板 Osteon lamella

间骨板 Interstitial lamella

number, the central to the right of the central tube, the central tube and pierced

tube are interlinked, layers of concentric arrangement of the bone plate around

the central canal、 There are some interstitial lamellae between osteon, which

has no vascular channels, in the bone plate or between bone plate visible yellow

small cavities, named lacuna、

实验三、肌组织与神经组织实验二

基本组织3

报告主题:心肌

主题属性:指定

标本号:12#

材料:人心肌切片

染色:HE染色

教学要求:观察人心肌得结构特点,掌握其判断依据

闰盘

Intercalated

disk

图3 心肌(人心肌切片,HE染色,40×100)

Figure 3 Cardiac muscle(human cardiac muscle smear,HE stained 40×100)

多数心肌纤维有一个细胞核,少数有双核,核呈卵圆形,位于细胞中央。心肌纤维得连接处称闰盘,且染色深。心肌纤维呈明暗相间得周期性横纹,但由于其肌原纤维粗细不

等,界限不明,因此横纹不如平滑肌那么清楚。

Most of the myocardial fibers have a nucleus, a few have dual-core, and nucleus is oval, located in the central cell、 The joint of the myocardial fibers called

intercalated disc, and deep dyeing、 The myocardial fibers are light and shade

and periodic horizontal lines, but as a result of its myofibril different

thicknesses of the unknown, so horizontal stripes as smooth muscle so clear 、

基本组织4

报告主题:多级神经元 主题属性:指定 标本号:9#

材料:小牛脊髓及神经节 染色:HE 染色

教学要求:观察脊髓前角多级神经元得结构特点,掌握其判断依据

图4 多级神经元(小牛脊髓切片,HE 染色,40×100)

多级神经元细胞核有大、圆,亮得特点,胞体与突起中可见到许多紫蓝色得不规则得小斑块,即尼氏体。突起从胞体发出,一个神经元有多个树突,一个轴突,因此轴突很难切到,不容易瞧见。Multistage neurons nuclei have big, round, bright characteristics, cell and bumps in the visible to many purple blue irregular small patches, namely the Nissl body 、 Flash from the cell body out, a neuron have multiple dendrite, an axon, so it is difficult to cut the axon, which is not easy to see 、实验四 消化管 中空性器官1

报告主题:空肠绒毛

尼氏体 Nissl body

树突 Dendrit

细胞核 Nucleus 神经胶质细胞得细胞核

主题属性:指定

标本号:19#

材料:猫空肠横切片

染色:HE染色

教学要求:掌握空肠得结构特征与判断依据,特别注意肠绒毛与肠腺得结构)

纹状缘

Striated border

单层柱状上皮

Simple

columnar

epithelium

平滑肌

Smooth muscle

图5 空肠绒毛(猫空肠横切片,HE 染色,40×100)

Figure5 jejunum villi(cat jejunum transverse section, HE stained 40×100)

空肠绒毛上皮由吸收细胞、杯状细胞与少量内分泌细胞组成。吸收细胞数量最多,呈高柱状,核长椭圆型,位于基部,细胞游离有深染层,称纹状缘。绒毛中轴得固有层为细密

结缔组织,其内常可见纵行得平滑肌、丰富毛细血管、1~2条中央乳糜管(多数未切到)及

大量淋巴细胞。

The epithelium of Jejunum villi is posed of absorption, goblet cell and a small amount of endocrine cells、 The largest number of absorptive cells, a tall

columnar, nuclear long elliptic, is located in the base, cell free have

hyperchromatic layer, called striated border、 Villi axis of the lamina propria

for fine connective tissue, it often visible longitudinal done smooth muscle,

rich capillaries, 1 ~ 2 a central lacteal (most not cut) and a large number of

lymphocytes、

实验五消化管以外得其她中空性器官

中空性器官2

报告主题:中等动脉 主题属性:指定 标本号:10#

材料:狗中等动、静脉横切片 染色:HE 染色

教学要求:掌握中等动脉得结构特点

jLhHP 。

图6 中等动脉 (狗中等动脉横切片,HE 染色, 40×100)

Figure 6 medium-sized artery (dog medium-sized artery and vein transverse section smear ,HE stained 40×100)在高倍镜下,可见内弹性膜为一条呈波浪状得折光性强得粉红色亮带,它就是内膜与中膜得分界标志,中膜较厚,由数十层平滑肌纤维组成,因此中动脉也称肌性动脉。外膜由疏松结缔组织构成,多数中动脉得外膜与中膜之间有明显得外弹性膜。At high magnification, internal elastic membrane is a wavy refraction sex strong pink light band, which is the border of tunica media and tunica adventitia 、tunica media is consist of dozens of layer of the smooth muscle fibers, so the artery is also called the muscular artery 、 External elastic membrane is posed of loose connective tissue, the majority of the artery; there is obvious elastic membrane between tunica media and tunica adventitia 、实验六 免疫系统

内膜 Tunica intima

中膜 Tunica media

外膜 Tunica adventitia

内弹性膜 Internal elastic Membrane

外弹性膜External elastic membrane

实质性器官1

报告主题:淋巴结

主题属性:指定

标本号:13#

材料:狗淋巴结切片

染色:HE 染色

被膜

Envelope

淋巴小结

Lymphoid

nodule

深皮质

Deep cortex

髓索

Medullary

cord

髓质

Medulla

图7 淋巴结 (狗淋巴结切片,HE染色 ,40×100)

Figure 7 Lymphoid node (Dog lymphoid smear , HE stained ,40×100)

淋巴结多呈扁平豆形或者卵圆形,表面有薄层结缔组织被膜,伸入实质形成小梁,小梁互相连接成网。皮质位于被膜下,浅皮质由淋巴小结与弥散淋巴组织构成,副皮质为胸

腺依赖区,为较大片得弥散淋巴组织,皮质淋巴窦位于被膜下方与小梁周围、髓质由髓索

及其间得髓窦构成。

Lymph node is flat bean shaped or ovoid, the surface has a thin layer of connective tissue capsule, stretch into substantial form trabecular, trabecular

interconnected into network、 Cortex is located in the envelope, shallow cortex

by lymph nodule and dispersion lymphoid tissue structure; vice cortex for thymus

depend on the area, for a large dispersion lymphoid tissue, cortex lymphatic sinus,

in the envelope below and trabecular meshwork、 Medulla around by pulp line and

the pulp sinus between positions、

实验八肾与内分泌系统

实质性器官2

报告主题:下颌下腺

主题属性:自选

标本号:24#

材料:人下颌下腺切片

染色:HE染色

教学要求:掌握下颌下腺得三种腺泡与导管得结构

浆液性腺泡

Serous acinus

分泌管

Secretary duct

半月(混合性

腺泡)

Demilune

黏液性腺泡

Mucous acinus

图8下颌下腺(人下颌下腺切片,HE染色,40×100)

Figure 8 (Submandibular gland smear, human submandibular gland,HE stained 40×100)

下颌下腺为混合性腺,小叶内可见很多圆形或不规则得腺泡,其中染紫红色得就是浆液性腺泡,染色浅得就是黏液性腺泡,混合性腺泡主要由黏液性腺细胞构成,少量浆液性

腺细胞位于黏液性细胞之间或聚集在腺泡底部,也称为半月。分泌管为单层柱状上皮,多

且发达,闰管短而不明显。

The submandibular gland is mixed gland, the leaves can be seen many round

or irregular acinus , including dye aborigine is serous acinus, dyeing shallow

is mucous acinus, mixed gland is mainly posed of mucous cells and a small amount

of serous cells in the slime sex between cells or gathered in gland bubble bottom,

called demilune、 Secretary duct for single columnar epithelium, and developed,

leap tube short is not obvious、

实验八肾与内分泌系统

实质性器官3

报告主题:肾单位

主题属性:指定

标本号:29#

材料:兔肾切片

染色:HE染色

教学要求:掌握肾单位各部分得结构特点

远曲小管Distal

convoluted

tubule

近曲小管

Proximal

convoluted

tubule

肾小囊

Renal capsule

血管球

Renal

glomerulus

图9 肾单位 (兔肾切片,HE染色, 40×100)

Figure 9 Nephron ( Rabbit kidney smear,HE stained ,40×100)

肾单位由肾小体与与其相连得肾小管组成,肾小体由血管球与肾小囊构成,血管球为肾小囊内一团蟠曲得毛细血管,肾小囊就是上皮性管道盲端膨大并凹陷得双层囊。近曲小

管位于肾小体周围,管径大,管壁厚,管腔小而且不规则。远曲小管与近曲小管混在一起,

管径小,管壁薄,管腔较大而规则。

Nephron is posed of renal corpuscle and renal tubule、 Renal corpuscle by vascular ball and renal capsule form, glomus for renal capsule in a group of

capillary, renal capsule is double capsule、 Proximal convoluted tubule in renal

corpuscle around, pipe diameter, wall thickness, the lumen is small and irregular、

Distal convoluted tubule and proximal convoluted tubule mixed together, pipe

diameter is small, and the wall is thin

实验九生殖腺

实质性器官4

报告主题:生精小管

主题属性:指定

标本号:41#

材料:人睾丸与附睾切片

染色:HE染色

教学要求:了解睾丸与附睾得一般结构,掌握生精小管得结构与间质细胞得位置及其结构特点。

精子

Spermatozoon

精子细胞

Spermatid

初级精母细胞

Primary

spermatocyte

精原细胞

Spermatogonium

支持细胞

Sustentacular

cell

图10 生精小管(人睾丸与附睾切片,HE染色,40×100)

Figure 10 Somniferous tubule (human testis and epididymis , HE stained ,40×100)

生精小管为高度弯曲得复层上皮性管道,外有薄层基膜,管壁由各级生精细胞与支持细胞构成。精原细胞附着在基膜上,胞体较小,核染色较深。初级精母细胞位于精原细胞近腔侧,细胞体积大而圆,核亦大而圆,染色质密集呈绒球状。次级精母细胞因存在时间短不易找到,精子细胞位于近腔面,体积更小,核大而圆,染色质细密。精子位于腔面,头部呈扁圆形,嵌入支持细胞得胞质中,染色深,尾部游离于生精小管腔内。

Somniferous tubules are curved stratified epithelium of pipeline, outside a thin layer of basement membrane, wall by various raw sperm cell and support cells position、 Spermatophore attached on the basement membrane, cell is lesser, nuclear staining deep、 Primary spermatocyte located in nearly cavity side, cell volume is big and round, nuclear also large, round, chromatin concentrated in wool ball、 Secondary spermatocyte there for short time is not easy to find, sperm cells in the nearly cavity surface, the volume is smaller, nuclear large, round, chromatin close、 Sperm is located in the cavity surface, the head is oblateness, embedded support cell cytoplasm, dyeing deep, tail free born in somniferous tubule cavity、

自选主题

实验二、结缔组织

基本组织5

报告主题:嗜酸性粒细胞

主题属性:自选

标本号:7#

材料:人血涂片

染色:Wright染色

教学要求:掌握嗜酸性粒细胞

红细胞

Erythrocyte

细胞核

Nucleus

嗜酸性颗粒

Eosinophilic

Granule

图11 嗜酸性粒细胞(人血涂片,HE染色,40×100)

Figure 11 Esoinophil (human blood smear,HE stained ,40×100)

嗜酸性粒细胞核多分为两叶,胞质内充满粗大、分布均匀、染成橘红色、有折光性得

嗜酸性颗粒。其主要作用就是能吞噬抗原抗体复合物,分解组胺,灭活白三烯,从而减

弱过敏反应,又参与对蠕虫得免疫反应。

Eosinophilic nuclear are mainly divided into two leaves, cytoplasm filled

with bulky, distribution uniformity, dyed orange, refraction sex

eosinophilic particles、 Its main function is to devour antigen antibody plex,

deposition of histamine, inactivated leukotriene, thereby deadening allergic

reaction, and the worm in the immune response、

实验一、上皮组织

基本组织6

报告主题:变移上皮

主题属性:自选

标本号:31#

染色:HE染色

材料:猫膀胱切片(空虚状态)

教学要求:掌握膀胱空虚状态下变移上皮得形态结构

盖细胞

Cover cells

基膜

Basement

membrane

图12 变移上皮 (猫膀胱切片,HE染色,40×100)

Figure 12 Transitional epithelium (cat bladder smear,HE stained ,40×

100)

变移上皮由多层细胞构成,基底面平整。细胞核常排成5~6层,其中,基底层细胞核密

集,小且染色深;中间数层细胞多为多边形或倒梨形,核圆;表层细胞大,分界清晰,有

防止尿液侵蚀得作用,称为盖细胞。

Transitional epithelium cells by multilayer structure, base with smooth

surface、 The nucleus is often in 5 ~ 6 layer, among them, the basal lamina

nuclei intensive, small and dyeing deep; Intermediate layers of cells for

polygon or fall pear-shaped, nuclear round; The surface cells, boundary clear,

have prevent urine erosion effect, called cover cell、

实验四、消化管

中空性器官3

报告主题:食管

主题属性:自选

标本号:18#

染色:HE染色

材料:狗食管与胃纵切片

教学要求:掌握食管得结构特点及判断依据

复层扁平上皮

Stratified

squamous

epithelium

粘膜肌层

Muscularis

mucosa

食管腺

Esophageal

glands

肌层

Muscularis

图13 食管 (狗食管与胃纵切片,HE染色,40×100)

Figure 13 Esophagus (dog esophagus and stomach longitudinal section, HE

stained ,40×100)

食管黏膜得上皮为复层扁平上皮,固有层为细密结缔组织,黏膜肌层由纵行平滑肌束

组成。黏膜下层得结缔组织中存在许多黏液性得食管腺。肌层由内环形与外纵行两

层,人得食管上段为骨骼肌,下段为平滑肌。外膜为纤维膜。

Esophageal mucosa epithelial is stratified squamous epithelium, Muscularis

mucosa is connective tissue, longitudinal beam of smooth muscle make up muscularis

mucosa 、 Submucosa of connective tissue has many esophageal glands、 musularis

from inner ring and outer longitudinal two layer, human esophageal upper segment

for skeletal muscle, lower segment for smooth muscle、 Tunica adventitia is fiber

membrane、

实验七、消化腺与肺

实质性器官5

报告主题:肝

主题属性:自选

标本号:26#

染色:HE染色

材料:香猪肝切片

教学要求:掌握肝得结构特点及其判断依据。

肝血窦

Hepatic

sinusoid

中央静脉

Central vein

门管区

Portal area

肝板

hepatic

plate

图14 肝 (香猪肝切片,HE 染色 ,40×100)

Figure 14 liver (xiang pig liver smear , HE stained ,40×100)

肝就是实质性器官,外有结缔组织被膜,伸入实质将其分为许多小叶。小叶中央为中

央静脉,以其为中心,肝板与肝血窦向周围呈放射状排列。肝板为肝细胞单层排列得

板状结构,肝血窦为肝板之间得间隙。在相邻小叶之间得结缔组织为门管区,其内可

见小叶间动脉、静脉及小叶间胆管。

Liver is substantive organs, the connective tissue capsule, and stretch into

essence which is divided into many lobules、 Centrilobular for central vein,

with it as the center, liver plate and hepatic sinusoids into the surrounding

radiated arrangement、 Liver plate for liver cell monolayer arranged plate

structure, hepatic sinusoids for liver board between clearances、 In between

the adjacent lobules connective tissue for portal area, its visible

interlobular arteries and veins and interlobular bile duct、

实验八、肾与内分泌系统

实质性器官6

报告主题:甲状腺

主题属性:自选

标本号:33#

染色:HE染色

材料:狗甲状腺切片

教学要求:掌握甲状腺得结构特点。

甲状腺滤泡

Thyroid follicle

滤泡上皮细胞

Follicular

epithelial cell

胶质

Colloid

图15 甲状腺(狗甲状腺切片,HE 染色,40×100)

Figure 15 thyroid (dog thyroid smear,HE stained ,40×100)

甲状腺为实质性器官,外有结缔组织被膜伸入实质将其分为多个小叶,小叶中可见许

多大小不一得滤泡,由滤泡上皮细胞围成,多为单层立方上皮,滤泡腔内充满均质嗜

酸性得胶质。滤泡间细胞团中可见有少量滤泡旁细胞。

Thyroid for substantive organs, the connective tissue capsule stretch into

essence which is divided into multiple lobules, lobules seen in many sizes

follicle, the follicle epithelial cells surrounded, for single cuboidal

epithelium and follicular cavity filled with homogeneous eosinophilic

colloid、 Follicle cells visible between the group with a small amount of

the follicle parietal cell、