Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineral

Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineralized

dentin matrix

Rui-Rui Liu;Ming Fang;Ling Zhang;Cheng-Fang Tang;Qi Dou;Ji-Hua Chen

【期刊名称】《国际口腔科学杂志（英文版）》

【年(卷),期】2014(000)003

【摘要】Our previous studies showed that biomodification of demineralized dentin collagen with proanthocyanidin (PA) for a clinically practical duration improves the mechanical properties of the dentin matrix and the immediate resin-dentin bond strength. The present study sought to evaluate the ability of PA biomodification to reduce collagenase-induced biodegradation of demineralized dentin matrix and dentin/adhesive interfaces in a clinically relevant manner. The effects of collagenolytic and gelatinolytic activity on PA-biomodified demineralized dentin matrix were analysed by hydroxyproline assay and gelatin zymography. Then, resin-/dentin-bonded specimens were prepared and challenged with bacterial collagenases. Dentin treated with 2%chlorhexidine and untreated dentin were used as a positive and negative control, respectively. Collagen biodegradation, the microtensile bond strengths of bonded specimens and the micromorphologies of the fractured interfaces were assessed. The results revealed that both collagenolytic and gelatinolytic activity on demineralized dentin were