Effects of dietary supplementation of seleniu
O R I G I N A L A R T I C L E
Effects of dietary supplementation of selenium-enriched
probiotics on production performance and intestinal microbiota of weanling piglets raised under high ambient temperature
C.H.Lv 1,T.Wang 2,N.Regmi 2,X.Chen 1,K.Huang 1and S.F.Liao 2
1Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls,Nanjing Agricultural University,Nanjing,Jiangsu,China,and 2Department of Animal and Dairy Sciences,Mississippi State University,Mississippi State,MS,USA
Summary
This study was designed to evaluate the ef?cacy of selenium-enriched probiotics (SeP)on production performance and intestinal microbiota of piglets raised under high ambient temperature.Forty-eight cross-bred weanling piglets (28days old),randomly allotted into 12pens (four piglets/pen)and four dietary treatments (three pens/treatment group),were fed ad libitum for 42days a basal diet (Con)or the basal diet supplemented with probiotics (Pro),sodium selenite (ISe)or a SeP preparation.Blood and faecal samples were collected on days 0,14,28and 42post-treatment.The SeP group had higher ?nal BW (p <0.05),greater ADG (p <0.05)and lower FCR (p <0.01)than the Pro,ISe or Con group.The diarrhoea incidence rate of either SeP or Pro group was lower (p <0.01)than the ISe or Con group.Blood Se concentration and GSH-Px activity were both higher (p <0.01)in the SeP than in the Pro,ISe or Con group.On days 28and 42,the serum concentrations of T3were higher (p <0.01)and T4lower (p <0.01)in the SeP than in the ISe group,and the T3and T4concentrations in the ISe group,in turn,were higher (p <0.05)and lower (p <0.01),respectively,than in the Pro or Con group.Also on days 28and 42,the faecal counts of lactobacillus bacteria were higher (p <0.01)while Escherichia coli lower (p <0.01)in the SeP or Pro group as compared to the ISe or Con group.The results of RFLP showed that the faecal microbial ?ora in the SeP group changed the most (numerically)as compared to the Pro or ISe group.These results suggest that the SeP product may serve as a better alternative to antibiotics than the solo probiotics for using as a growth promoter for weanling piglets.
Keywords direct-fed microbials,selenium,swine,growth performance,faecal microbiota,glutathione peroxidase,thyroid hormone
Correspondence S.F.Liao,PO Box 9815,Mississippi State University,Mississippi State,MS 39762,USA.Tel:+1-662-3257318;Fax:+1-662-3258873;E-mail:sliao@https://www.wendangku.net/doc/8b11232841.html, and K.Huang,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China.Tel:+86-25-84395507;Fax:+86-25-84398669;E-mail:khhuang@https://www.wendangku.net/doc/8b11232841.html, Received:12August 2014;accepted:3March 2015
Introduction
Heat stress is a critical factor that negatively affects swine production performance and welfare.The harmful effects of heat stress on top of weaning stress can cause lethargy,anorexia,decrease in feed ef?-ciency,weight loss,and high morbidity and mortality in piglets,which in turn leads to prolonged production cycle and enormous economic loss in swine industry (Stahly et al.,1979;Fuquay,1981;McGlone and Pond,2003).Furthermore,as is known,environmen-tal and nutritional changes both can dramatically alter the gastrointestinal microbial population (Burkholder et al.,2008;Torrallardona et al.,2012),decrease the immune functions and damage the antioxidant defence system of weanling pigs (Morrow-Tesch et al.,1994).To minimize the harmful effects of weaning and heat stresses on piglet’s health and production
performance,probiotics and selenium (Se)have been postulated to be alternatives to antibiotic growth pro-moters.
Probiotics (a.k.a.direct-fed microbials)refer to viable micro-organisms (bacteria or yeast)that are harmless to the host but promote a bene?cial balance of the autochthonous gastrointestinal microbial population (Fuller,1989;Sharma and Devi,2014).Studies in human and animals have shown that oral administra-tion of probiotics can prevent pathogen proliferation,alleviate in?ammatory stress,improve digestive func-tion of the intestinal tract and stimulate the immune system of the host via maintaining a balanced intestinal microbiota (Fuller,1989;Jin et al.,2000;Steidler et al.,2000;Meng et al.,2010;Sharma and Devi,2014).Because of the public pressure and bans on the use of antibiotic growth promoters in animal feeding practice,probiotics have been suggested as the most desirable
DOI:
10.1111/jpn.12326
alternative for livestock production due to their bene?-cial effects(Shim et al.,2005;Meng et al.,2010). Selenium is an essential trace element nutrient that plays an important role in host health maintenance through certain selenoproteins such as glutathione peroxidase(GSH-Px)family and50-iodotyrosine deio-dinase type I.The effect of inorganic versus organic sources of Se(ISe vs.OSe)on heat stress has been conducted in a pilot study with broiler chickens, which indicated that OSe had advantages over ISe in enhancing animal glutathione-GSH-Px redox system and reducing animal oxidative stress caused by mild heat exposure(Mahmoud and Edens,2003).
Our laboratories have been developing a series of Se-enriched probiotics(SeP)products that may have combined effects of probiotics and Se for the use as feed additives to promote animal health and produc-tion performance.Several animal experiments on these SeP products have been conducted,and the resulted bene?cial effects have been reported(Qin et al.,2007;Pan et al.,2011;Ren et al.,2011;Ibrahim et al.,2012).In laying hens,it was found that dietary SeP supplementation increased laying rate,egg weight,egg Se content and egg GSH-Px activity and decreased the feed:egg ratio and the egg total choles-terol content(Pan et al.,2011).Feeding SeP to dogs and lambs increased their antioxidant status and blood Se concentration and enhanced the composition of gut bacterial?ora(Qin et al.,2007;Ren et al.,2011). The hypothesis for this study was that this new SeP product would have better bene?cial effect than the traditional probiotic products in terms of promoting pig production performance because of the additive effect between OSe and probiotics.The objectives of this study were to evaluate the ef?cacy of a new SeP product on(i)the production performance,(ii)the blood antioxidative and thyroid hormone status and (iii)the intestinal bacterial population,of weanling piglets raised under high ambient temperature. Materials and method
Preparation of selenium-enriched probiotics
Two probiotic strains of micro-organisms,Lactobacillus acidophilus(strain number AS1.1878,from the Insti-tute of Microbiology,Chinese Academy of Sciences) and Saccharomyces cerevisiae(strain number NAU-GH-SE1,from the Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls,Nanjing Agricultural University,China),were successfully pre-pared into one probiotics product and one SeP product (Chinese patent no.ZL200510040990.2;Pan et al., 2011).Brie?y,these two strains were cultured in 200ml MRS(de Man,Rogosa and Sharpe)and
200ml YEPD(yeast extract peptone dextrose)liquid
media,respectively,at32°C for24h for the ampli?-
cation of L.acidophilus and S.cerevisiae.While the
YEPD broth was composed of20g/l D-glucose,10g/l
peptone and5g/l yeast extract,the MRS broth was
composed of20g/l D-glucose,10g/l peptone,5g/l
yeast extract,10g/l beef extract,5g/l sodium acetate,
2g/l triammonium citrate,1ml of Tween80,0.58g/l
MgSO4á7H2O,0.05g/l MnSO4á4H2O and2g/l K2HPO4.Both the solid MRS medium and the solid
YEPD medium were prepared by adding1.5%agar
and used for the culture and enumeration of L.
acidophilus and S.cerevisiae respectively.
Based on the results of our preliminary study(K.
Huang and C.Pan,unpublished data),YEPD broth
was selected as the fermentative medium for both
strains.The concentration of ISe(in a form of sodium
selenite)added to the fermentation medium was
0.3mg/kg.Cells from the MRS and YEPD cultures
were inoculated into YEPD fermentation medium at
5%(v/v)level.The fermentative setting was estab-
lished as inoculation volume5%,culture time36h,
shaking speed100rpm and further anaerobical incu-
bation at37°C for48h.After recovery,the colony-
forming units(CFU)of L.acidophilus and S.cerevisiae
strains in both products(Pro and SeP)were approxi-
mately1011and109CFU/ml respectively.The total Se
content in SeP product was approximately10.0mg/l,
with over90%of the total Se being OSe and over
75%being selenomethionine(Gao et al.,2006).For
determination of OSe amount,the SeP culture was
centrifuged and total Se amounts in the supernatant
and the microbial sediment were both determined.
The Se in the supernatant was considered as ISe and
that in the sediment was considered as OSe.
Animal trial procedure and sample collection
The protocol of animal trial was approved by the
Committee for the Care and Use of Experimental
Animals of Nanjing Agriculture University,and the
trial was conducted in a semi-intensive swine farm
near Taizhou City(Jiangsu,China)during a hot
summer.Forty-eight cross-bred(Landrace9York-
shire9Duroc)weanling piglets whose individual
body weight(BW)was7.9?0.2kg at the age of
28days were randomly allotted into12feeding pens
(four piglets/pen),which were housed in a semi-open
barn in the farm.Pigs were allowed ad libitum access
to clean water and their respective treatment diets
during the whole period of the trial,which lasted for
42days.The average daily temperature was38°C
Effects of selenium-enriched probiotics on piglets C.H.Lv et al.
during the42-day trial period(35°C during the?rst 33days and40°C during the last9days).
The trial was conducted with four dietary treatments with individual feeding pens serving as experimental units and three pens serving as three replicates for each treatment.The four dietary treatments were as follows: (i)the control group(Con)fed a basal diet(Table1), (ii)the ISe group fed the basal diet supplemented with sodium selenite,(iii)the probiotics group(Pro)fed the basal diet supplemented with the probiotics product and(iv)the SeP group fed the basal diet supplemented with the SeP product.As shown in Table1,the basal diet contained0.16mg intrinsic total Se per kilogram. The ISe and SeP supplements elevated the dietary total Se level to0.46mg/kg.Both the probiotics and SeP-supplemented diets contained live L.acidophilus and S.cerevisiae at391011and39109CFU/kg respec-tively.All diets met or exceeded the nutrient require-ments as suggested by NRC(1998).
On days0,14,28and42post-initiation of the die-tary treatments,blood samples were collected by acute venipuncture of anterior vena cava of individual piglets between2:00and4:00pm.Two pigs from each pen were randomly selected for blood collection.One whole-blood sample from each pig was sent to the lab-oratory for immediate determination of GSH-Px activ-ity,and another whole-blood sample was used for Se concentration determination.One serum sample was prepared for each pig by letting the blood tube to stand in a slanting position for2h at ambient room temperature,followed by centrifugation at3000g for 15min.All serum samples were stored atà20°C until laboratory analysis of thyroid hormones.Fresh faecal samples were collected aseptically at8:00am on the same days from two randomly selected pigs of each pen and kept at4°C for<24h before they were sent to the laboratory for microbiota analysis. Determination of animal production performance
The BW of each individual pigs was taken at the begin-ning(day0)and also at the end(day42)of the animal trial period,and thus,the average daily gain(ADG)was calculated for the42-day period.The average daily feed intake(ADFI)was also calculated based on the total feed consumption during the42-day period.The feed conversion ratio(FCR)was calculated as ADFI:ADG. Pig faeces were examined on a daily basis for calcu-lating the rate of diarrhoea incidence for each treat-ment group,which was expressed as a percentage(%) of the total diarrhoea incidence for a given group(i.e. the diarrhoea counts during the whole42days) divided by the total possible diarrhoea counts for all the pigs,which is the product of the total number of the piglets(i.e.48)and the total experimental days (i.e.42days).The highest possible rate of incidence of each treatment group was25%.A faecal scoring sys-tem previously used by Healey et al.(2003)was adopted for counting diarrhoea incidence,and except for score1(normal solid faeces),all scores2–4were counted as diarrhoea.
Laboratory analyses of blood and faecal samples
The total Se concentrations of blood were determined using an AF-610A atomic?uorescence spectrometer (Ruili Analysis Instrument Co.,Beijing,China) according to the method described previously by Pan et al.(2007).The Se stock standard solution of sodium selenite[GBW(E)080215]and the certi?ed Se refer-ence material,pork liver[GBW08551],were provided by the National Research Center for Standard Materi-als(Beijing,China)and the Ministry of Commerce Food Detection Science Institute(Beijing,China) respectively.The water used in the chemical analyses was of ultrapure grade(resistance18MΩ/cm).
The whole-blood GSH-Px activities were determined using a commercial assay kit purchased from Nanjing Jiancheng Bioengineering Institute(Catalog No.A005; Jiangsu,China)in accordance with the manufacturer’s instructions.The serum concentrations of thyroid hor-
Table1Ingredient composition and nutrient analysis of the basal diets
(as fed basis)
Ingredient Content(%)Nutrient Content
Corn60.25Digestible energy
(kcal/kg)
3421
Soya bean meal22.50Metabolizable
energy(kcal/kg)
3168
Whey powder 5.50Crude protein(%)19.5
Fish meal 5.50Ca(%)0.88
Soya bean oil 2.50Available
phosphorus(%)
0.67
Mono-Cal* 1.15Fe(mg/kg) 1.34
Limestone0.85Se(mg/kg)0.16
Salt0.15Lysine(%) 1.40
Lysine-HCl0.38Methionine(%)0.46
Methionine0.12Cystine(%)0.33
Threonine0.07Threonine(%)0.88
Tryptophan0.03Tryptophan(%)0.25
Mineral–vitamin
premix?
1.00
*Mono-Cal,Calcium monohydrogen phosphate.
?Provided per kilogram of diet:Mn60mg,Fe130mg,Zn156mg,Cu
45mg,vitamin A11,025IU,vitamin D32,203IU,vitamin E80IU,vita-
min K4.4mg,vitamin B14.4mg,vitamin B211mg,vitamin B335mg,
vitamin B4330mg,vitamin B559.5mg,vitamin B110.9mg,vitamin H
0.5mg and vitamin B1255l g.
C.H.Lv et al.Effects of selenium-enriched probiotics on piglets
mones including triiodothyronine(T3)and thyroxine
(T4)were measured using Iodine[125I]3,30,5-Triiodo-
thyronine Radioimmunoassay Kit and Iodine[125I]
Thyroxine Radioimmunoassay Kit,respectively,fol-
lowing the manufacturer’s instructions(North Biotech-
nology Research Institute,Beijing,China).
The bacterial community of the faecal samples was
enumerated by various culture methods.Brie?y,one
gram of faecal sample from each pig was diluted by
10-fold serials until10à69with phosphate-buffered saline(PBS:137m M NaCl, 2.7m M KCl,10m M
Na2HPO4,2m M KH2PO4),and aliquots of100l l dilu-
tion were individually plated onto different selective
agar media which were supplied from a commercial
source(Qingdao Hope Bio-Technology,Co.Ltd.,
Shandong,China).The bacterial culture,colony iden-
ti?cation and enumeration were also conducted
according to the manufacturer’s instructions.Escheri-
chia coli were enumerated on EMB agar,lactobacilli on
MRS agar,bi?dobacteria on BLB agar,staphylococcus
on Vogel johnson(USP)agar and enterococcus on
mENT agar(Yang et al.,2009).Faecal cultures were
incubated at37°C,and lactobacilli and bi?dobacteria
were performed in an anaerobic cabinet within
24–48h.Each concentration gradient was performed
in triplicate.After culture,the number of faecal bacte-
ria was counted,and?nally,the bacterial population
was expressed as a log of the CFU/g on the plate.
PCR-Restriction fragment length polymorphism analysis
Faecal samples(approximately1g each)were washed
three times by suspension in3.0ml of aseptic PBS(pH
7.2)and centrifuged at300g for5min to remove the
impurities and PCR inhibitors.Subsequently,the super-
natant was centrifuged at3500g for10min and the fae-
cal pellets were extracted and puri?ed using the Column
Stool DNAout according to the manufacturer’s instruc-
tions(Tiandz,Beijing,China).The?nal faecal bacterial
DNA samples were stored atà20°C until analysis.
A fragment of the prokaryotic16S rDNA gene was
ampli?ed from the faecal DNA extracts by PCR using
primers speci?c to conserved sequences?anking vari-
able regions V3,V4and V5:50-CTACGGGAGGCAG-
CAGT-30(forward)and50-CCGTCWATTCMTTTGAGT
TT-30(reverse)(high-pressure liquid chromatography-
puri?ed;Invitrogen,Shanghai,China).Primers and
PCR conditions followed those described by Lane
(1991).All reactions were performed in0.2-ml tubes
with a Takara Thermal Cycler Dice TP600(Takara
Biotechnology Co.,Dalian,China)in a?nal volume of
0.05ml.Each reaction contained25ng of DNA,1m M MgCl2,1l M each of the primers,25l l premix (Takara).The thermal cycle programme for the reac-tion consisted of1cycle at94°C(for4min)and35 cycles at94°C(for1min),45°C(for1min)with an increment of1°C per cycle and72°C(for75s),fol-lowed by one?nal extension step at72°C(for 15min).After visual con?rmation of the PCR products with agarose gel electrophoresis,four restriction enzymes(Hha I,Alu I,Msp I and Afa I)were used, respectively,for the restriction fragment length poly-morphism(RFLP)analysis.The digestions were carried out for2h independently with appropriate restriction buffers at the recommended temperature according to the manufacturer’s instructions(Takara).Different fragments were separated using a2%agarose gel with a Tris-acetate buffer and a100-bp ladder.Gels were stained with Goldview and photographed with a Bio-Rad ChemiDoc XRS+(Bio-Rad,Hercules,CA,USA). The degree of microbial diversity was measured as the total number of different bands obtained from the four independent restriction digestions on each sam-ple(Castillo et al.,2006).The size and the intensity of the bands within each lane of a gel were analysed by the Quantity One1-D Analysis Software(Bio-Rad), and the degree of microbial biodiversity was expressed as the total number of different bands obtained from the four independent restriction digestions.For pair-wise comparisons of the banding patterns and the construction of dendrograms,similarity matrices were generated based on the Manhattan distance that takes into account the size and the intensity of the bands generated(Kaufmann and Rousseeuw,1990). Statistical analysis
The differences among the dietary treatments for the aforementioned parameters were analysed by one-way analysis of variance using SPSS statistical software(for windows;version17.0;SPSS Inc.,Armonk,NY,USA). Feeding pens were used as experimental units(n=3). The data for the faecal bacteria community were log transformed before statistical analysis.To separate the means among dietary treatments,Duncan’s multiple range tests were used,and p≤0.05was considered sta-tistically signi?cant unless indicated otherwise. Results
Animal production performance
While there were no differences in the initial BW and no differences in the ADFI among the four dietary treatment groups,the piglets in the SeP group had higher ADG(p<0.01)and?nal BW(p<0.05)than
Effects of selenium-enriched probiotics on piglets C.H.Lv et al.
the piglets in the Pro,the ISe or the Con group (Table2).Although the?nal BW of the ISe and Pro groups was not statistically higher than that of the Con group,the ADG of both ISe and Pro groups was higher (p<0.05)than that of the Con group.On the contrary to the pattern of the ADG differences among the four groups,the FCR of the SeP group was lower(p<0.01) than that of the Pro or ISe group(p<0.05),and the FCR of both Pro and ISe groups was further lower (p<0.05)than that of the Con group.There was no difference in the ADFI among the four groups.
The rate of diarrhoea incidence of the ISe group was lower(p<0.01)than that of the Con group(Table2). The rate of incidence of either the Pro group or the SeP group was further lower(p<0.01)than that of the ISe group,while there was no difference in the rate of incidence between the SeP and the Pro groups. Blood selenium concentration and glutathione peroxidase activity
On day0,there was no difference in blood Se concen-trations among the four dietary treatment groups (Table3).On day14,28or42post-treatment,the blood Se concentrations in the SeP group were higher (p<0.01)than that in the ISe group,which in turn was higher(p<0.01)than that in the Pro or the Con group,whereas no difference was found between the Pro and the Con groups on those days.
On day0,there was no difference in blood GSH-Px activities among the four dietary treatment groups (Table3).On day14,28,or42post-treatment,the blood GSH-Px activities in the SeP group were higher (p<0.01)than those in the ISe group,which in turn was higher(p<0.01)than that in the Pro or the Con group,whereas no difference was found between the Pro and the Con groups on those days.Serum thyroid hormone concentration
As can be seen in Table4,on day0or14,there were no differences among the four dietary treatment groups in either the serum T3or the serum T4concen-tration.On day28or42,the piglets in the SeP group had higher T3concentration(p<0.01)and lower T4 concentration(p<0.01)than the piglets in the ISe group,and the T3and T4concentrations in the ISe group,in turn,were higher(p<0.05)and lower (p<0.01),respectively,than those in the Pro or Con group.There were no difference between the Pro and the Con groups in either the T3or the T4concentra-tion on day28or42post-treatment.
Faecal microbial population
On day0or14post-treatment,there were no differ-ences among the four dietary treatment groups in the population of lactobacillus,bi?dobacterium, E.coli, enterococcus or staphylococcus bacteria(Table5).On days28and42post-treatment,although there was no difference between the SeP and the Pro groups in the population of lactobacillus,the populations of lactoba-cillus in both groups were higher(p<0.01)than the population in either the ISe or the Con group.Also on days28and42,although there was no difference between the SeP and the Pro groups in the population of E.coli,the population of E.coli in both groups was lower(p<0.01)than the population in either the ISe or the Con group.No differences were observed among the four dietary treatment groups in the popu-lations of bi?dobacterium,enterococcus or staphylo-coccus bacteria on day28or42post-treatment. Three faecal samples from each of the four treat-ment groups on day42post-treatment were analysed using the PCR-RFLP analysis method.For each pen,
Table2Effect of Se-enriched probiotics on the production performance of the weanling piglets
Items Dietary Treatment*
Con ISe Pro SeP SEM?
Initial BW,kg7.947.857.837.790.13 Final BW,kg26.7a28.5a28.5a30.8b0.56 ADG,g447a491b493b548c10.7 ADFI,g94596395795526.4 FCR 2.11c 1.98b 1.94b 1.74a0.02 Diarrhoea incidence,%15.6c11.3b9.52a8.53a0.68
Means within a row that have a different superscript letter differ(p<0.05or0.01).
*Con represents the control group fed the basal diet;ISe represents the inorganic Se group fed the basal diet supplemented with sodium selenite pro-viding additional0.3mg Se per kilogram of diet;Pro represents the probiotics group fed the basal diet supplemented with the probiotics product pro-viding live L.acidophilus and S.cerevisiae at391011and39109CFU per kilogram of diet respectively;SeP represents the selenium-enriched probiotics group providing additional0.3mg Se per kilogram of diet,and live L.acidophilus and S.cerevisiae at391011and39109CFU per kilogram of diet respectively.
?SEM represents the pooled standard error of the means(n=3).
C.H.Lv et al.Effects of selenium-enriched probiotics on piglets
one faecal sample was randomly selected from the two samples collected from the given pen on day 42post-treatment.The PCR-ampli?ed V3,V4and V5regions of the 16S rDNA were digested by Hha I,Afa I,Alu I and Msp I restriction enzymes.The numbers of bands,which were considered as the degree of micro-bial biodiversity,were 25.3,24.6,21.1and 20.3for the pigs in the SeP,Pro,ISe and Con groups respec-tively (Fig.1).Although the increase in the number of bands for the SeP group (25.3vs.20.3)was not sta-tistically signi?cant (p >0.05),the effects of dietary treatments on microbial composition were clearly dis-tinguished by the cluster analysis.A dendrogram com-paring different banding patterns (Fig.2)clearly illustrates four distinct clusters,which are associated
Table 3Effect of Se-enriched probiotics on the blood Se concentration and glutathione peroxidase activity of the weanling piglets
Day
Dietary treatment*
Con
Pro
ISe
SeP
SEM ?
Blood Se concentration (l g/l)0156.5157.1155.8
156.6
0.4214157.9a 158.4a
165.7b
177.7c 0.9428164.7a 166.3a
176.5b 194.5c 1.0442167.3a 168.5a 190.6b 202.8c 0.91Blood glutathione peroxidase activity (U/l)0192.7191.4191.1
191.0 1.7314214.4a 217.7a
295.8b 322.4c 1.9028235.9a 239.3a 417.9b 442.5c 2.4842293.3a 301.3a 523.8b
550.3c
4.23
Means within a row that have a different superscript letter differ (p <0.01).
*Con represents the control group fed the basal diet;ISe represents the inorganic Se group fed the basal diet supplemented with sodium selenite providing additional 0.3mg Se per kilogram of diet;Pro rep-resents the probiotics group fed the basal diet supplemented with the probiotics product providing live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively;SeP represents the selenium-enriched probiotics group providing addi-tional 0.3mg Se per kilogram of diet,and live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively.
?SEM represents the pooled standard error of the means (n =3).
Table 4Effect of Se-enriched probiotics on serum thyroid hormone concentrations (ng/ml)of the weanling piglets
Day Hormone ?Dietary treatment*
Con Pro ISe SeP SEM ?0T30.870.940.930.930.06T473.068.469.069.0 1.3614T30.940.970.98 1.020.05T474.972.178.276.6 1.3828T30.96a 1.01a 1.08b 1.19c 0.02T471.1c 69.4c 63.9b 58.2a 0.4742
T30.54a 0.58a 0.69b 0.89c 0.02T4
61.7c
60.0c
52.4b
48.8a
0.70
Means within a row that have a different superscript letter differ (p <0.05or 0.01).
*Con represents the control group fed the basal diet;ISe represents the inorganic Se group fed the basal diet supplemented with sodium selenite providing additional 0.3mg Se per kilogram of diet;Pro rep-resents the probiotics group fed the basal diet supplemented with the probiotics product providing live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively;SeP represents the selenium-enriched probiotics group providing addi-tional 0.3mg Se per kilogram of diet,and live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively.
?T3stands for triiodothyronine,and T4stands for thyroxine.?SEM represents the pooled standard error of the means (n =3).
Table 5Effect of Se-enriched probiotics on the intestinal microbiota (log CFU/g)of the weanling piglets
Bacteria
Dietary treatment*
Con
ISe
Pro
SeP
SEM ?
Day 0
Lactobacillus 8.398.538.548.510.09Bi?dobacterium 8.238.018.068.110.10Escherichia coli 7.747.657.677.690.10Enterococcus 6.47 6.42 6.59 6.530.07Staphylococcus 5.59 5.56 5.63 5.550.09Day 14
Lactobacillus 7.837.737.767.820.13Bi?dobacterium 8.027.927.817.880.09Escherichia coli 7.977.957.857.880.14Enterococcus 7.027.217.127.170.13Staphylococcus 6.02 6.09 6.15 5.980.13Day 28
Lactobacillus 8.48a 8.38a 9.22b 9.27b 0.12Bi?dobacterium 8.238.288.398.560.12Escherichia coli 7.83b 7.78b 6.73a 6.65a 0.09Enterococcus 6.72 6.8 6.99 6.950.08Staphylococcus 5.52 5.56 5.54 5.470.11Day 42
Lactobacillus 8.40a 8.25a 9.25b 9.33b 0.10Bi?dobacterium 8.228.188.468.30.08Escherichia coli 7.85b 7.78b 6.87a 6.73a 0.09Enterococcus 6.64 6.76 6.76 6.690.13Staphylococcus
5.84
5.77
5.75
5.68
0.09
Means within a row that have a different superscript letter differ (p <0.01).*Con represents the control group fed the basal diet;ISe represents the inorganic Se group fed the basal diet supplemented with sodium sele-nite providing additional 0.3mg Se per kilogram of diet;Pro represents the probiotics group fed the basal diet supplemented with the probiot-ics product providing live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively;SeP represents the selenium-enriched probiotics group providing additional 0.3mg Se per kilogram of diet,and live L.acidophilus and S.cerevisiae at 391011and 39109CFU per kilogram of diet respectively.
?SEM represents the pooled standard error of the means (n =3).
Effects of selenium-enriched probiotics on piglets C.H.Lv et al.
with four dietary treatments.The similarities between the SeP and the Con groups,between the ISe and the Con groups and between the Pro and the Con groups were 65.0%,74.6%and 68.1%respectively.Discussion
Blood selenium content and glutathione peroxidase activity
Selenium is a structural component in the function centre of GSH-Px,and thus,the GSH-Px activities are strongly associated with the Se status in the body of an animal or human (Bobcek et al.,2004).In the present study,we found that the blood GSH-Px activ-ity and Se concentration were higher in the SeP or ISe group as compared to the Pro or Con group.Similar result was also found in growing-?nishing pigs by Mahan et al.(1999)who reported that both serum Se concentration and GSH-Px activity were increased when Se (in the form of Se-enriched yeast or sodium selenite)was fed.This present study also found that both blood GSH-Px activity and Se concentration in the SeP group were higher than those in the ISe group,which is also in agreement with the results reported by Mahan et al.(1999),who found that the effect of Se-enriched yeast was more robust than that of sodium selenite.In ?nishing lambs,Qin et al.(2007)also reported that OSe was more effective than ISe in increasing blood Se concentration and GSH-Px activity.Similar results were also reported in beef cat-tle by Liao et al.(2011).
It is known that heat stress can lead to the genera-tion of lipoperoxides and free radicals (e.g.O à2and
HO ˙),which can in turn damage cell membranes by
inducing lipid peroxidation of polyunsaturated fatty acids in the membranes (Laudicina and Marnett,1990).Glutathione and GSH-Px redox system has the ability to protect cell membrane integrity by extin-guishing free radicals that cause lipid oxidation (Meister and Anderson,1983;Palmer and Paulson,1997).Zhao and Guo (2005)proved that Se supple-mentation can increase the activities of superoxide dismutase (SOD)and GSH-Px while decrease malondialdehyde (MDA)content and the free radical damages induced by heat stress in pigs.Mahmoud and Edens (2003)and Pan et al.(2011)also found that chickens fed OSe (vs.ISe)had higher blood GSH-Px activity and higher blood capacity to reduce the oxi-dized glutathione increased by heat stress.
The greatest blood Se concentration and GSH-Px activity associated with the dietary SeP supplementa-tion found in this study suggests that the SeP product used is highly effective in lifting the blood antioxida-tive capacity of pigs.
Blood thyroid hormones
Thyroid contains more Se than any other tissues,and Se de?ciency aggravates myxedematous cretinism and autoimmune thyroid disease in humans (Duntas,2010).Recent recognition of Se incorporation in all three deiodinases has decisively con?rmed an unam-biguous link between Se and thyroid function (Duntas,2010).T4,the major thyroid prohormone,is converted to the more metabolically active hormone,T3,by type I and type II iodothyronine
deiodinases
Fig.1Example of gel electrophoresis of the PCR-ampli?ed V3,V4and V5regions of the 16S rDNA digested by Msp I,Afa I,Alu I,and Hha I restriction enzymes.Four lanes represent four treatment groups of piglets receiving the basal diet (Con),the basal diet supplemented with sodium selenite (ISe),the basal diet supplemented with the probiotics product (Pro)or the basal diet supplemented with selenium-enriched probiotics product (SeP).Refer to the section of Materials and Methods for the de?nition of each treatment group.
C.H.Lv et al.Effects of selenium-enriched probiotics on piglets
(€Un
€u san,2001).Se de?ciency impairs thyroid hor-mone metabolism by inhibiting the synthesis and activity of iodothyronine deiodinases and the conver-sion of T4to T3(€Un
€u san,2001).As is known,thyroid hormones play important roles in animal growth performance and animal adaptation to environmental challenges,as T3and T4can increase the basal metabolic rate,enhance glucose utilization,modify lipid metabolism and stimulate protein synthesis (Todini et al.,2007).Previous stud-ies found that the levels of the two thyroid hormones were decreased,and the function of thyroid was inhibited due to high environmental temperature or hyperthermia in summer (Baccari et al.,1983;Tuckova et al.,1995;Mustafa et al.,2008;Melesse et al.,2011).This present study showed that the serum T3level was increased while T4level was decreased in the SeP or ISe group than in the Con or Pro group of pigs on days 28and 42post-treatment.This result is in agreement with the point of view that Se supplementation can affect peripheral thyroid metabolism (Arthur et al.,1992;Jianhua et al.,2000;Gursu et al.,2003).The alteration of thyroid hormone levels in the present study might be a result from the altered deiodinase activity due to Se supplementation,which suggests that dietary Se may exert bene?cial effects on thyroid hormone status in weanling pigs under high ambient temperature.The highest serum T3concentration and lowest T4concentration of the SeP group of pigs in this study suggest that more T4molecules were converted to T3molecules,which
should be one of the reasons responsible for the great-est production performance of the SeP group of pigs.The differences between the SeP and ISe groups should be attributed to the chemical form of Se.
Faecal microbial ecosystem
The gastrointestinal microbiota forms a complex eco-logical system that exerts speci?c trophic,metabolic and protective functions for the host (Guarner and Ma-lagelada,2003).The biodiversity and stability of this ecosystem bene?t not only animal nutrient utilization,but also animal health status and production perfor-mance (Jensen,1998).Early studies showed that stressful conditions could alter gastrointestinal micro-bial population (i.e.microbiota),which leads to detri-mental effects on the host (Fuller,1989).Oral administration of probiotics can modulate gastrointesti-nal microbiota by increasing the number of speci?c bacteria and thus change the microbiota composition.Shim et al.(2005)reported that feeding probiotics decreased the number of total coliform bacteria and increased the number of bi?dobacteria in the gastroin-testinal tract of suckling pigs.Marshall-Jones et al.(2006)reported that feeding L.acidophilus (DSM13241strain)to healthy cats increased the numbers of lacto-bacillus and L.acidophilus groups and decreased the numbers of Clostridium spp and Enterococcus faecalis in cats’faeces.Biagi et al.(2007)fed freeze-dried prepara-tion of L.animalis (LA4strain,isolated from the faeces of a healthy dog)to adult dogs for 10days and found that faecal lactobacilli count was increased,faecal enterococci showed a trend towards a reduction and L.animalis appeared in all faecal samples collected.Similarly,this study found that the lactobacillus count was higher while the E.coli count was lower in the Pro or SeP group than in the ISe or Con group on days 28and 42post-treatment.
The PCR-RFLP analysis of the 16S rDNA gene from faecal extraction was performed in this study to explore the biodiversity of the pig faecal microbiota.Based on the results of DNA ?ngerprinting pro?les,the differ-ences in the number of bands and the type of bands should be related to the changes in the biodiversity of the microbiota.Castillo et al.(2006)using the RFLP method comparing the biodiversity of gastrointestinal microbiota from early weaned pigs fed avilamycin,butyrate and plant extracts and found that the butyrate treatment resulted in a more complex microbial com-munity (increased bands)with a more robust intestinal environment.Montesi et al.(2005)evaluated the effect of probiotics in caecal microbiota of rats using the DGGE (denaturant gradient gel electrophoresis)technique
and
Fig.2Dendrogram of linkage illustrating the similarity (in percentage)of PCR-RFLP banding patterns for the different treatment groups.The dendrogram represents results from 12piglets killed on day 42post-treatment.Cluster C represents the Con group,cluster SS represents the ISe group,cluster P represents the Pro group and cluster SP repre-sents the SeP group (refer to the section of Materials and Methods for the de?nition of each treatment group).
Effects of selenium-enriched probiotics on piglets C.H.Lv et al.
found that the number of16S rRNA gene fragments was increased after fed the rats with probiotics.In this study,the number of RFLP bands in piglets fed SeP was more(numerically)than those with other three dietary treatments,suggesting a more complex and robust micro-bial ecosystem associated with the SeP supplementation. Animal production performance
The effect of heat stress on pigs is evident in low feed consumption,loss of weight and decreased production ef?ciency(St-Pierre et al.,2003).When weanling pig-lets are exposed to high ambient temperature,they will suffer from impaired health,disordered gastroin-testinal microbiota,diarrhoea and even death under a combination of different stress factors(Sutherland et al.,2006).Earlier studies on avian species have shown that supplementation of diets with Se or probi-otics is a measure to reduce the problems associated with heat stress and to improve the birds’health status and production performance(Zulki?i et al.,2000;Sa-hin and Kucuk,2001;Lan et al.,2004).Haddad and Goussous(2005)found that yeast culture(as probiot-ics)supplementation of high-energy diets fed to?n-ishing lambs improved lambs’weight gain,ADG and FCR.However,Mahan et al.(1999)did not?nd any effect of dietary supplementation of selenium-enriched yeast on ADG and FCR in growing-?nishing pigs.
In this study,piglets were raised under high ambi-ent temperature(average daily temperature ranging from35to40°C).Although there was only numerical increase in the?nal BW of pigs in the ISe or the Pro group when compared to the pigs in the Con group, the ADG of pigs in the ISe or the Pro group was higher than that in the Con group.The dietary supplementa-tion of SeP increased the ADG and the?nal BW of the piglets when compared to other three groups (Table2).The FCR of the piglets fed the SeP-supple-mented diet was lower than those of piglets fed the Pro supplemented,the ISe supplemented or the con-trol diet.These results suggest that the SeP used in this study is more effective than the ISe or probiotics on the production performance of the piglets under a heat stress.
The bene?cial effect of SeP on the piglets’production performance could be linked to the enhanced blood an-tioxidative capacity,the enhanced thyroid function and the healthier gastrointestinal ecosystem,as dis-cussed above.The robust effect of SeP product on pig-let’s production performance could also be linked to the decreased diarrhoea incidence in the SeP group when compared to the other three groups.It is known that probiotics may compete with harmful micro-organisms for adhesion sites,produce some compounds that inhibit pathogens,enhance immune response (Verschuere et al.,2000)and decrease diarrhoea inci-dence(Castillo et al.,2006)of the host.Huang et al. (2004)reported that the complex lactobacilli prepara-tion improved the balance of the gastrointestinal mic-robiota and enhanced the pigs’resistance to E.coli infection.Taras et al.(2007)reported that Enterococcus faecium and Bacillus cereus can reduce the incidence of post-weaning diarrhoea in pigs.
L.acidophilus and S.cerevisiae can reduce pH, improve digestive capacity and reduce the risk of gas-trointestinal disorders in swine,poultry and dairy calves(Cruywagen et al.,1996;Lin et al.,2007).An acidic condition in the gut may be in part responsible for the antibacterial effects on the harmful enterobac-teria.In addition,Se supplementation can also pre-vent Se-de?ciency diarrhoea(Thomas et al.,1994), and this may partially explain the low diarrhoea inci-dence rate of the piglets fed the Se diet when com-pared to the piglets fed the Con diet.The diarrhoea incidence rate of the piglets fed the SeP diet was the lowest among the four treatment groups,suggesting that the SeP product may be used as a better antibiotic alternative to control the intestinal disorder of the pig-lets exposed to heat stress,when comparing to solo probiotics.
Conclusion
The results generated from this study indicated that dietary SeP supplementation can enhance antioxidative capacity,enhance thyroid function,pro-duce a more stable and healthy gastrointestinal eco-system,decrease diarrhoea incidence rate and improve the production performance of post-weanling piglets raised under high ambient temperature.This conclusion suggests that the SeP product developed in these laboratories may serve as a better probiotic feed additive for using as a growth promoter for weanling pigs,especially under heat stress. Acknowledgement
This work was supported by the National Natural Sci-ence Foundation of China(grant number31272627), the Priority Academic Program Development of Ji-angsu Higher Education Institutions(Jiangsu,China) and the research fund from Mississippi Agricultural and Forestry Experiment Station(grant numbers 027*********and027*********;approved for pub-lication as Journal Article No.12571).
C.H.Lv et al.Effects of selenium-enriched probiotics on piglets
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C.H.Lv et al.Effects of selenium-enriched probiotics on piglets
大学生毕业晚会主持词
大学生毕业晚会主持词 A：青春是一曲荡气回肠的歌，三年前我们怀着彩色梦想走进了鄂大，三年中我们有过欢笑，流过泪水，经历磨炼，得到成长 B：三年后的今天，我们在这里重温青春过往，因为明天就将各奔天涯。也正因为此，今夜，我们承载了太多的祝福与惦念，寄托了太多的关怀与企盼。 C：今晚，让我们再一次重温，那些感动过我们的人和事：灯火通明的教学楼里用功的身影，篮球场上捍卫集体尊严的男儿霸气，满是欢声笑语的宿舍边不着边际的闲谈…… D：又一个三年轮回之后，在同样微凉的夏夜，你是否会记起校园里的梧桐树，你是否会记起日记本里的书签，那些五彩缤纷的日子？ A：无数个三年之后，你们的思念是否会有增无减？在你成长的岁月里，在你闯荡社会的每一天，是否会有那么几个瞬间让你渴望回到过去 B：让我们静静享受一下挑战，在日复一日与时间的赛跑中，你会变得更加坚强，更加神采奕奕！又一个青春之旅从今晚启航，又一个光阴的故事在今晚讲述 C：亲爱的同窗，不要带着离别的愁绪，因为明天又是一个新的起点，因为我们相信再次相逢，我们还是一首动人的歌！D：很高兴今天能有这个机会同大家相聚一堂，共叙离别。就让今天铭刻在我们心间，让母校留住我们的风采！ A：今天，我们也非常荣幸的请到了…… B：下面让我们用热烈的掌声有请……上台讲话 结束语A：欢快的舞蹈表达不尽我们对母校的敬意 B：热情的赞歌唱不尽我们对母校的一腔深情
C：流火的六月，我们将带着恩师的叮咛，怀着必胜的信心，走向新的征程 D：绚烂的七月，我们将载着母校的祝愿，带着亲人的希望，向着新的征程扬帆起航A：老师们，同学们，欢送10级毕业生联欢晚会合：到此结束 A李：毕业，是一个沉重的动词； 刘：毕业，是一个让人一生难忘的名词； 李：毕业，是感动时流泪的形容词； 刘：毕业，是当我们以后孤寂时候，带着微笑和遗憾去回想时的副词； 李：毕业，是我们夜半梦醒，触碰不到而无限感伤的虚词。 刘：若干年后，假如我们还能够想起那段时光，也许这不属于难忘，也不属于永远，而仅仅是一段记录了成长经历的回忆。 李：尊敬的各位领导老师 刘：亲爱的各位同学们 合：大家晚上好！ 李：很荣幸和大家相聚在这激情如火的六月，在这充满忧伤的六月！ 刘：很高兴和大家相聚在“放心去飞，20年后再相聚—毕业晚会”现场！ 李：我是李扬 刘：我是刘伟清 李：今天晚会现场非常荣幸的邀请到院系的各位领导和老师们。 刘：让我们首先欢迎…… 李：灿烂的星空曾经铭刻你我的笑容
湘艺版五年级音乐《长白山下的歌谣》教学设计
湘艺版五年级音乐《长白山下的歌谣》 教学设计 《长白山下的歌谣》教学设计 教材版本：湘艺版音乐教材 教学对象：五年级上期 一、教学案例： （一）题：《长白山下的歌谣》音乐综合 （二）教材版本：湘版新标实验教材 （三）年级：五年级 （四）教具准备：钢琴、AI、打击乐器、朝鲜族服饰、长鼓、金达莱花。 （五）教学目标： ①、认知目标：认真听赏朝鲜民歌《道拉基》，学习三拍子节奏特点。 ②、能力目标：通过小组合作学习（设计身势节奏、用长鼓为歌曲伴奏、即兴歌舞等形式）培养学生的创新能力、合作能力，体验朝鲜族民歌的韵律。 ③、情感目标：通过感受性学习歌曲《金达莱花开朵朵红》，了解朝鲜族人民勤劳淳朴、团结互助、热情好客、能歌善舞的优良传统与民族风情。 （六）设计思路： 五十六个民族五十六枝花，生活在吉林省长白山脚下的
朝鲜族，其歌舞有着独特的节奏韵律。湘版新标实验教材五年级上册第八单元题为：《长白山下的歌谣》，它引起了我的研究兴趣。本单元的教育主题为：用民族音乐激发学生的爱国情感，实现多元文化的交流。即：通过音乐去感受民族文化，进而去了解民族文化；反之，通过文化，我们才可以去体验民族音乐，整体地去认识民族音乐。本时选用听赏《桔梗谣》和演唱歌曲《金达莱花开朵朵红》这两部分内容，教学设计的基本思路是：使学生通过多种形式听、唱“长白山下的歌谣”，深刻体验民族音乐“原生态”的美，以及其歌舞文化、思想内涵；在师生平等互动的学习氛围里，通过实践创作、对歌曲的再表现等形式，激发学生热爱民族音乐、热爱我们伟大祖国的情感。 堂教学中我以“新程”为指导思想，对教学设计进行了一些新的尝试。以下结合自己教学实践中的堂教学个案，和大家共同评析与探讨。 （七）教学过程 ᠄ 导入新过程：即“看风景”。 、AI播放《大长今》影片，背景音乐:《大长今》的主题曲《希望》。 （学生起立，跟随老师自由律动。） 师：刚才同学们听到的旋律出自？
大学毕业晚会主持词四人
大学毕业晚会主持词四人 四个主持人主持的大学毕业晚会应该怎么说主持词呢下面小编跟大家分享几篇大学毕业晚会主持词，以供参考! 大学毕业晚会主持词一 1.节奏引领时尚，余音环绕广场，新鲜的韵律，高难度的声响，一样为您缔造不一样的完美想象，下面请欣赏来自07音乐班的xxx为您带来的《》相信会给您带来意外的惊喜。 2.铃声清脆鼓铿锵，天山铃舞尽展情，来自天山的自然是蝶飞花影动，美丽各不同，那就让我们停下脚步，一饱眼福，欣赏由xxx为我们带来的舞蹈《天山铃舞》 3. 军中有歌，歌才动情，军旅里飞来一只熟悉的百灵，不错下面就由请xxx为大家献上一首耳熟能详的老歌，《军中飞来一只百灵》 4.炫酷才叫时尚，八零后心之向往，相信大家都一样，需要个性张扬。今天我们特意请来了城市建设学院的武状元优秀团队，为大家献上一段充满活力的XXX，感谢他们的到来，大家掌声欢迎! 5.相信大家都看过一部曾经风靡一时的电影，那是周杰伦曾经推出的一部年度巨献《不能说的秘密》.里面的四手联弹一定给大家留下了不可磨灭的深刻印象，旋律依旧在脑中回响。不过经典一样可以复制，精彩一样能够粘贴，下面给大家带来的这个惊喜，可谓是非常具有特色，一样是所见
不多，机会难得。下面请欣赏xxxx为大家带来的，钢琴四手连弹《军队进行曲》 6.幽幽云水意.漫漫古典情. 诗情画境的晕染为我们带来流动的娴静。请大家随着动人的舞蹈穿越书法的妙境，伴随优雅的琴韵体会超越喧嚣的古韵墨香。下面请欣赏xxx为您带来的xxxx 7.四年的岁月流光见证了你我在辽东学院的欢欣成长，相信由很多即将走出校园的同学都想倾诉衷肠，下面我们就请出来自05对外汉语的毕业生代表xxx听听她如何表达。 8.当你离开的时候，我送走你的只有沉默。当你离开我的时候，我绝不会把泪滴落。这是金达莱花的歌词大意，相信朝鲜族金达莱花的舞姿会像歌词一样优美，让人回味。下面请欣赏xxxx金达莱花。 9.是谁带来远古的呼唤，是谁留下千年的期盼，这首歌自打年初就开始被人重新无数次的翻唱，不过下面的歌曲，同样青藏高原的演唱，绝对会给您用实力带来不一样。由请xxxx 10.因为有爱，我们才牵手，因为有爱，我们才会风雨同舟。与身边爱自己的人共同牵手，一同遨游，幸福生活才能没有尽头。下面欣赏牵手。 11. 我对你的敬仰之情,如滔滔江水,连绵不绝;又如黄河之水,一发不可收拾;相信大家对电影《月光宝盒》中这一
精彩的大学毕业晚会主持词示范文格式本推荐
精彩的大学毕业晚会主持词示范文格式本推荐 Ａ：青春是一曲荡气回肠的歌，三年前我们怀着彩色梦想走进了鄂大，三年中我们有过欢笑，流过泪水，经历磨炼，得到成长Ｂ：三年后的今天，我们在这里重温青春过往，因为明天就将各奔天涯。也正因为此，今夜，我们承载了太多的祝福与惦念，寄托了太多的关心与企盼。 Ｃ：今晚，让我们再一次重温，那些感动过我们的人和事：灯火通明的教学楼里用功的身影，篮球场上保卫集体尊严的男儿霸气，满是欢声笑语的宿舍边不着边际的闲谈…… Ｄ：又一个三年轮回之后，在同样微凉的夏夜，你是否会记起校园里的梧桐树，你是否会记起日记本里的书签，那些五彩缤纷的日子? Ａ：无数个三年之后，你们的思念是否会有增无减?在你成长的岁月里，在你闯荡社会的每一天，是否会有那么几个霎时让你渴望回到过去 Ｂ：让我们静静享受一下挑战，在日复一日与时间的赛跑中，你会变得更加坚强，更加神采奕奕!又一个青春之旅从今晚启航，又一个光阴的故事在今晚讲述 Ｃ：亲爱的同窗，不要带着离别的愁绪，因为明天又是一个新的起点，因为我们相信再次相逢，我们还是一首动人的歌!Ｄ：很快乐今天能有这个时机同大家相聚一堂，共叙离别。就让今天铭刻在我们心间，让母校留住我们的风采!
Ａ：今天，我们也非常荣幸的请到了…… Ｂ：下面让我们用热烈的掌声有请……上台讲话 结束语Ａ：愉快的舞蹈表达不尽我们对母校的敬意 Ｂ：热情的赞歌唱不尽我们对母校的一腔深情 Ｃ：流火的六月，我们将带着恩师的叮咛，怀着必胜的信心，走向新的征程 Ｄ：绚烂的七月，我们将载着母校的祝愿，带着亲人的希望，向着新的征程扬帆起航 Ａ：教师们，同学们，欢送10级毕业生联欢晚会合：到此结束Ａ李：毕业，是一个沉重的动词; 刘：毕业，是一个让人一生难忘的名词; 李：毕业，是感动时流泪的形容词; 刘：毕业，是当我们以后孤寂时候，带着浅笑和遗憾去回想时的副词; 李：毕业，是我们夜半梦醒，触碰不到而无限感伤的虚词。 刘：若干年后，假设我们还能够想起那段时光，也许这不属于难忘，也不属于永远，而仅仅是一段记录了成长经历的回忆。 李：尊敬的各位领导教师 刘：亲爱的各位同学们 合：大家晚上好! 李：很荣幸和大家相聚在这激情如火的六月，在这充满忧伤的六月!
金达莱花 谐音
na bu gei ma ya kiu 那不给妈呀久儿我 ga xi yi dai gei no 噶西带给路 ma lou xi bu yi mu lei du gei you li da 吗楼西不一木类度给有大 na bu gei ma ya kiu 那不给妈呀久儿我 ga xi yi dai gei no 噶西带给路 qiu kou lo a ni nu mu nu li you li na 求口咯啊你奴木奴里又里那 na dou na han bu ka qi 那都那汗不卡气 yi jian gu dai a ni ji 一见古带啊你几 ku dai pa la bu miao 苦带怕拉不秒 ca la wu dai ka 擦拉屋带卡 ku niao ti yi ka niao ji 苦鸟提一卡鸟几 ca la gu a gu mi no mu kou 擦拉古啊古米闹木口 cu wu xi wu s ka wo so 粗屋西屋丝卡我收 ku dai han bu ka gei 苦带汗不卡给 yi lu jiur gei you 一路酒给又 nei you mu nu lo 内有木奴咯 yi lu jiu gei you 一路就给又 na bu gei ma ya kiu 那不给妈呀久我 ga xi yi dai gei no 噶西带给路 ma lou xi bu yi mu lei du gei you li da 吗楼西不一木类度给有里大yang gou li ya sang jin dang nei gu wu 羊够里呀桑金当内古 a long da ma de xi gei lei gu li you li da 啊龙大吗的西给类古里有里大ka xi le gong long no yin ku kou qiu 卡西了工龙木因哭口求 na bu li qiu liu ma pu ma xi you so sou 那不里求留吗扑吗西有否搜 na bu gei ga ya piao ka xi dei you nu 那不给妈呀久我噶西带给路 qiu kou lo a ni nu mu nu li you li na 求口咯啊你奴木奴里又里那 nei ga dou na ba leng dei you 内噶都那吧冷给又 ku dai lu man du la wu 苦带路满度啦舞 ku dan ku miao sa la han gei ji 苦但苦秒撒拉汗给几 na bu gei ma ya kiu 那不给妈呀久我 ga xi yi dai gei no 噶西带给路 ma lou xi bu yi mu lei du gei you li da 吗楼西不一木类度给有里大 yang gou li ya sang jin dang nei gu wu 羊够里呀桑金当内古 a long da ma de xi gei lei gu li you li da 啊龙大吗的西给类古里有里大ka xi le gong long no yin ku kou qiu 卡西了工龙木因哭口求 na bu li qiu liu ma pu ma xi you so sou 那不里求留吗扑吗西有否搜na bu gei ga ya piao ka xi dei you nu 那不给妈呀久我噶西带给路 qiu kou lo a ni nu mu nu li you li na 求口咯啊你奴木奴里又里那 na bu gei ga ya piao ka xi dei you nu 那不给妈呀久我噶西带给路 qiu kou lo a ni nu mu nu li you li na 求口咯啊你奴木奴里又里那
主持词 2020年大学毕业晚会主持词
xx年大学毕业晚会主持词 b：让我们静静享受一下挑战，在日复一日与时间的赛跑中，你会变得更加坚强，更加神采奕奕!又一个青春之旅从今晚启航，又一个光阴的故事在今晚讲述 c：亲爱的同窗，不要带着离别的愁绪，因为明天又是一个新的起点，因为我们相信再次相逢，我们还是一首动人的歌!d：很高兴今天能有这个机会同大家相聚一堂，共叙离别。就让今天铭刻在我们心间，让母校留住我们的风采! a：今天，我们也非常荣幸的请到了 b：下面让我们用热烈的掌声有请上台讲话 结束语a：欢快的舞蹈表达不尽我们对母校的敬意 b：热情的赞歌唱不尽我们对母校的一腔深情 c：流火的六月，我们将带着恩师的叮咛，怀着必胜的信心，走向新的征程 d：绚烂的七月，我们将载着母校的祝愿，带着亲人的希望，向着新的征程扬帆起航 a：老师们，同学们，欢送10级毕业生联欢晚会合：到此结束a李：毕业，是一个沉重的动词; 刘：毕业，是一个让人一生难忘的名词; 李：毕业，是感动时流泪的形容词; 刘：毕业，是当我们以后孤寂时候，带着微笑和遗憾去回想时的副词;
李：毕业，是我们夜半梦醒，触碰不到而无限感伤的虚词。 刘：若干年后，假如我们还能够想起那段时光，也许这不属于难忘，也不属于永远，而仅仅是一段记录了成长经历的回忆。 李：尊敬的各位领导老师 刘：亲爱的各位同学们 合：大家晚上好! 李：很荣幸和大家相聚在这激情如火的六月，在这充满忧伤的六月! 刘：很高兴和大家相聚在放心去飞，20年后再相聚毕业晚会现场! 李：我是李扬 刘：我是刘伟清 李：今天晚会现场非常荣幸的邀请到院系的各位领导和老师们。 刘：让我们首先欢迎 李：灿烂的星空曾经铭刻你我的笑容 刘：美丽的海岸曾经珍藏你我的背影。 李：连绵起伏的山川，像一双腾飞的翅膀支撑起我们坚韧不屈的信念， 刘：沸腾的黄海血脉，像千万奔涌的旋律连绵成我们亘古不灭的传说。 李：在这个光荣同梦想交融的时刻，让我们共同祈祷 合：环保的明天更美好!
毕业生晚会主持稿
毕业生晚会主持稿 毕业生晚会主持稿(一) A：青春是一曲荡气回肠的歌，三年前我们怀着彩色梦想走进了鄂大，三年中我们有过欢笑，流过泪水，经历磨炼，得到成长：三年后的今天，我们在这里重温青春过往，因为明天就将各奔天涯。也正因为此，今夜，我们承载了太多的祝福与惦念，寄托了太多的关怀与企盼。 C：今晚，让我们再一次重温，那些感动过我们的人和事：灯火通明的教学楼里用功的身影，篮球场上捍卫集体尊严的男儿霸气，满是欢声笑语的宿舍边不着边际的闲谈 D：又一个三年轮回之后，在同样微凉的夏夜，你是否会记起校园里的梧桐树，你是否会记起日记本里的书签，那些五彩缤纷的日子? A：无数个三年之后，你们的思念是否会有增无减?在你成长的岁月里，在你闯荡社会的每一天，是否会有那么几个瞬间让你渴望回到过去 ：让我们静静享受一下挑战，在日复一日与时间的赛跑中，你会变得更加坚强，更加神采奕奕!又一个青春之旅从今晚启航，又一个光阴的故事在今晚讲述 C：亲爱的同窗，不要带着离别的愁绪，因为明天又是一个新的起点，因为我们相信再次相逢，我们还是一首动人的歌!D：很
高兴今天能有这个机会同大家相聚一堂，共叙离别。就让今天铭刻在我们心间，让母校留住我们的风采! A：今天，我们也非常荣幸的请到了 ：下面让我们用热烈的掌声有请上台讲话 结束语A：欢快的舞蹈表达不尽我们对母校的敬意 ：热情的赞歌唱不尽我们对母校的一腔深情 C：流火的六月，我们将带着恩师的叮咛，怀着必胜的信心，走向新的征程 D：绚烂的七月，我们将载着母校的祝愿，带着亲人的希望，向着新的征程扬帆起航 A：老师们，同学们，欢送10级毕业生联欢晚会合：到此结束 A李：毕业，是一个沉重的动词; 刘：毕业，是一个让人一生难忘的名词; 李：毕业，是感动时流泪的形容词; 刘：毕业，是当我们以后孤寂时候，带着微笑和遗憾去回想时的副词; 李：毕业，是我们夜半梦醒，触碰不到而无限感伤的虚词。 刘：若干年后，假如我们还能够想起那段时光，也许这不属于难忘，也不属于永远，而仅仅是一段记录了成长经历的回忆。 李：尊敬的各位领导老师 刘：亲爱的各位同学们
大学生精彩毕业晚会主持词
大学生精彩毕业晚会主持词 大学生精彩毕业晚会主持词 A：六月、江畔的微风情深意暖，校园涌动的花海流溢飘香。 B：六月、想起毕业带来的奔忙，满怀即将失去的无限感伤。 C：六月、拾起曾经蕴藏的过往，体味相伴四年的欢欣成长。 D：六月、让你我懂得了珍惜，珍惜在校的每一段岁月流光。 A：毕业，是一沉重的动词，行动里带着眷恋与不舍。 B：毕业，是一难忘的名词，话语里带着心酸与苦涩。 C：毕业，是一流泪的形容词，不可或缺但又不能推辞。 D：毕业，是个遗憾的副词，历练成长却又让生活充实。 A：四年前，大家相遇相知，温暖胸膛、怀揣梦想。 B：四年里，大家共同努力，激越青春、欢乐成长。 C：四年后，即将天各一方，各自打造梦想的天堂。 D：朋友们，不要让泪水打湿容颜，火热激情即将绽放当晚，让我们的心于空中翱翔，在青春的蓬勃下生长，在岁月流光中温暖启航。 A：下面我宣布，20XX年师范学院青春启航文化节之毕业生晚会正式开始。 1.节奏引领时尚，余音环绕广场，新鲜的韵律，高难度的声响，一样为您缔造不一样的完美想象，下面请欣赏来自XX音乐班的xxx为您带来的《》相信会给您带来意外的惊喜。
2.铃声清脆鼓铿锵，天山铃舞尽展情，来自天山的自然是蝶飞花影动，美丽各不同，那就让我们停下脚步，一饱眼福，欣赏由xxx为我们带来的舞蹈《天山铃舞》 3. 军中有歌，歌才动情，军旅里飞来一只熟悉的百灵，不错下面就由请xxx为大家献上一首耳熟能详的老歌，《军中飞来一只百灵》 4.炫酷才叫时尚，八零后心之向往，相信大家都一样，需要个性张扬。今天我们特意请来了城市建设学院的武状元优秀团队，为大家献上一段充满活力的XXX，感谢他们的到来，大家掌声欢迎! 5.相信大家都看过一部曾经风靡一时的电影，那是周杰伦曾经推出的一部年度巨献《不能说的秘密》.里面的四手联弹一定给大家留下了不可磨灭的深刻印象，旋律依旧在脑中回响。不过经典一样可以复制，精彩一样能够粘贴，下面给大家带来的这个惊喜，可谓是非常具有特色，一样是所见不多，机会难得。下面请欣赏xxxx为大家带来的，钢琴四手连弹《军队进行曲》 6.幽幽云水意.漫漫古典情. 诗情画境的晕染为我们带来流动的娴静。请大家随着动人的舞蹈穿越书法的妙境，伴随优雅的琴韵体会超越喧嚣的古韵墨香。下面请欣赏xxx为您带来的xxxx 7.四年的岁月流光见证了你我在XX学院的欢欣成长，相信由很多即将走出校园的`同学都想倾诉衷肠，下面我们就请出来自05对外汉语的毕业生代表xxx听听她如何表达。
校园艺术节主持词开场白
校园艺术节主持词开场白（一） 男：尊敬的各位领导、各位来宾。 女：亲爱的老师们、同学们。 合：大家好！ 男：聆听十月的讯息，秋风吹拂大地； 女：走进十月的校园，我们充满活力！ 男：今天，我们相聚这儿，绽放灿烂的笑容；凝聚我们的勇气； 女：今天，我们相聚这儿，放飞我们的希望，追求我们的梦想！ 男：校园的艺术是知识的一种新的升华，校园的生活是多彩的音乐的合奏。 女：校园艺术节是师生展示各自文艺风采、凸显个性魅力的一个最佳舞台。 合：xx中学第x届校园艺术节开幕式现在开始！（鸣礼炮） 男：下面进行升旗仪式，请仪仗队入场。 女：请全体起立，升国旗、奏国歌！ 男：请大家入座。今天来到我们会场的领导有：xx、xx、xx…… 女：让我们用热烈的掌声对他们的到来表示欢迎！ 男：另外许多家长代表也来到了我们的会场，一个成功的学生背后总有辛勤付出的家长，您辛苦了！ 女：让我们用热烈的掌声对他们表示感谢！ 男：回首我们走过的这一个学年，经过全校师生上下一心、卓有成效的努力，我校各项工作都取得了骄人的成绩：校容校貌焕然一新，常规管理扎实有效，教学质量跃上新高。
女：成绩已然过去，面对未来，我们信心百倍。下面有请x校长讲话。 （鼓掌感谢x校长的精彩致辞） 环节一：教师合唱《走进新时代》、《团结就是力量》 男：我们xx人是这样的热情奔放！ 女：我们xx人是这样的团结激昂！ 男：我们的手牵在一起，就是战胜困难的力量！ 女：我们的心连在一起，就是坚不可摧的铜壁铁墙！ 男：让我们与优美同行，与高雅同行，与时代同行 女：请欣赏教师合唱《走进新时代》和《团结就是力量》。 指挥：xx 领唱：xx 环节二：舞蹈《开门红》 女：扬起你欢乐的嘴角，带上你的好心情，在这个特别的日子里跟我们一起感受这花样年华。
毕业典礼晚会主持词
毕业典礼晚会主持词X 花开花落，又是一年毕业季，我们毕业典礼就要开始举办，下面是搜集整理的毕业典礼晚会主持词，欢迎阅读。更多资讯请继续关注毕业典礼栏目! 毕业典礼晚会主持词(一) A：青春是一曲荡气回肠的歌，三年前我们怀着彩色梦想走进了鄂大，三年中我们有过欢笑，流过泪水，经历磨炼，得到成长 B：三年后的今天，我们在这里重温青春过往，因为明天就将各奔天涯。也正因为此，今夜，我们承载了太多的祝福与惦念，寄托了太多的关怀与企盼。 C：今晚，让我们再一次重温，那些感动过我们的人和事：灯火通明的教学楼里用功的身影，篮球场上捍卫集体尊严的男儿霸气，满是欢声笑语的宿舍边不着边际的闲谈…… D：又一个三年轮回之后，在同样微凉的夏夜，你是否会记起校园里的梧桐树，你是否会记起日记本里的书签，那些五彩缤纷的日子? A：无数个三年之后，你们的思念是否会有增无减?在你成长的岁月里，在你闯荡社会的每一天，是否会有那么几个瞬间让你渴望回到过去 B：让我们静静享受一下挑战，在日复一日与时间的赛跑中，你会变得更加坚强，更加神采奕奕!又一个青春之旅从今晚启航，又一个光阴的故事在今晚讲述 C：亲爱的同窗，不要带着离别的愁绪，因为明天又是一个新的起点，因为我们相信再次相逢，我们还是一首动人的歌!D：很高兴今天能有这个机会同大家相聚一堂，共叙离别。就让今天铭刻在我们心间，让母校留住我们的风采! A：今天，我们也非常荣幸的请到了…… B：下面让我们用热烈的掌声有请……上台讲话
结束语 A：欢快的舞蹈表达不尽我们对母校的敬意 B：热情的赞歌唱不尽我们对母校的一腔深情 C：流火的六月，我们将带着恩师的叮咛，怀着必胜的信心，走向新的征程 D：绚烂的七月，我们将载着母校的祝愿，带着亲人的希望，向着新的征程扬帆起航 A：老师们，同学们，欢送10级毕业生联欢晚会合：到此结束 A李：毕业，是一个沉重的动词; 刘：毕业，是一个让人一生难忘的名词; 李：毕业，是感动时流泪的形容词; 刘：毕业，是当我们以后孤寂时候，带着微笑和遗憾去回想时的副词; 李：毕业，是我们夜半梦醒，触碰不到而无限感伤的虚词。 刘：若干年后，假如我们还能够想起那段时光，也许这不属于难忘，也不属于永远，而仅仅是一段记录了成长经历的回忆。 李：尊敬的各位领导老师 刘：亲爱的各位同学们 合：大家晚上好! 李：很荣幸和大家相聚在这激情如火的六月，在这充满忧伤的六月! 刘：很高兴和大家相聚在“放心去飞，20年后再相聚—毕业晚会”现场! 李：我是李扬