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DAPI染色液说明书

DAPI染色液

DAPI染色液说明书

产品简介:

DAPI染色液(DAPI Staining Solution)是经过精心优化几乎适用于所有常见细胞和组织细胞核染色的染色液。

DAPI,即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,也称DAPI dihydrochloride,分子式为C16H15N5 · 2HCl ,分子量为350.25 ,CAS Number 28718-90-3。

DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。

DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。

DAPI的最大激发波长为340nm,最大发射波长为488nm;DAPI和双链DNA结合后,最大激发波长为364nm,最大发射波长为454nm。

本DAPI染色液可以直接用于固定细胞或组织的细胞核染色。

DAPI染色液说明书

保存条件:

-20℃避光保存,一年有效。

注意事项:

本DAPI 染色液的浓度经过碧云天的优化,确保可以满足各种常规染色的需要。如需使用特定浓度的DAPI,请选购碧云天的DAPI(C1002)。

荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。

为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。

DAPI对人体有一定刺激性,请注意适当防护。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:

1.对于细胞或组织样品,固定后,适当洗涤去除固定剂。随后如果需要进行免疫荧光染色,则先进行免疫荧光染色,染色

完毕后再按后续步骤进行DAPI染色。如果不需要进行其它染色,则直接进行后续的DAPI染色。

2.对于贴壁细胞或组织切片,加入少量DAPI染色液,覆盖住样品即可。对于悬浮细胞,至少加入待染色样品体积3倍的染

色液,混匀。

3.室温放置3-5分钟。

4.吸除DAPI染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。

5.直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时,会看到凋亡细胞的细胞核呈致密浓染,或呈碎

块状致密浓染。

使用本产品的文献:

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Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis.

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Taurine protects transformed rat retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction. Brain Res. 2009 Jul 7;1279:131-8. Epub 2009 May 7.

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Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.

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Clarifying the signal network of salvianolic acid B using proteomic assay and bioinformaticanalysis.

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14.Zheng Y, Ren PP, Wang JL, Wang YF.

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15.Li H, Ma G, Gui D, Zhao S, Wang P, He K, Wang X, Ruan J, Cao J, Yang S, Li K.

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Mol Immunol. 2011 Mar;48(6-7):914-23.

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18.Yang XZ, Dou S, Sun TM, Mao CQ, Wang HX, Wang J.

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19.Zhao J, Yu H, Lin L, Tu J, Cai L, Chen Y, Zhong F, Lin C, He F, Yang P.

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29.Hu X, Chen Z, Mao X, Tang S

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30.Jiang F, Liu T, He Y, Yan Q, Chen X, Wang H, Wan X

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31.Li X, Chen D, Le C, Zhu C, Gan Y, Hovgaard L, Yang M.

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32.Shen X, Wang C, Zhu Q, Li T, Yu L, Zheng W, Fei C, Qiu M, Xue F

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Vet Parasitol. 2012 Apr 30;185(2-4):290-5.

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A biodegradable amphiphilic and cationic triblock copolymer for the delivery of siRNA targetingthe acid ceramidase gene for cancer therapy. Biomaterials. 2011 Apr;32(11):3124-33.

34.Wang Y, Xu H, Liu X, Liu L, Liang Z

Inhibition of fibroblast differentiation of muscle-derived stem cells in cell implantation treatment ofstress urinary incontinence.

Cell Reprogram. 2011 Oct;13(5):459-64.

35.Yan P, Li T, Bo M, Die L, Xing L

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J Cell Physiol. 2011 Nov;226(11):2834-40

37.Cheng J, Wang J, Chen B, Xia G, Cai X, Liu R, Ren Y, Bao W, Wang X.

A promising strategy for overcoming MDR in tumor by magnetic iron oxide nanoparticles co-loaded with daunorubicin and 5-bromotetrandrin.

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39.Shen L, Xu W, Li A, Ye J, Zhou J.

JWA enhances As?O?-induced tubulin polymerization and apoptosis via p38 in HeLa and MCF-7 cells.

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40.Gan Q, Dai D, Yuan Y, Qian J, Sha S, Shi J, Liu C.

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41.Zhang SJ, Ye F, Xie RF, Hu F, Wang BF, Wan F, Guo DS, Lei T.

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Id1-induced inhibition of p53 facilitates endothelial cell migration and tube formation by regulatingthe expression of beta1-integrin.

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Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targetingMMP16.

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51.Wang SB, Shi Q, Xu Y, Xie WL, Zhang J, Tian C, Guo Y, Wang K, Zhang BY, Chen C, Gao C, Dong XP.

Protein Disulfide Isomerase Regulates Endoplasmic Reticulum Stress and the Apoptotic Processduring Prion Infection and PrP Mutant-Induced Cytotoxicity. PLoS One. 2012;7(6):e38221.

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microRNA-124 Regulates Cardiomyocyte Differentiation of Bone Marrow-Derived MesenchymalStem Cells via Targeting STAT3 Signaling.

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53.Li S, Yu S, Zhang C, Shu H, Liu S, An N, Yang M, Yin Q, Yang H.

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MicroRNA-199a targets CD44 to suppress the tumorigenicity and multidrug resistance of ovariancancer-initiating cells.

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55.Li Q, Yan Z, Li F, Lu W, Wang J, Guo C

The improving effects on hepatic fibrosis of interferon-γ liposomes tar geted to hepatic stellatecells.

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56.Qu KX, He ZX, Nie WH, Zhang JC, Jin XD, Yang GR, Yuan XP, Huang BZ, Zhang YP, Zan LS.

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A comparative study of mouse nasal septal and turbinal epithelium for in vitro cell cultures.

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59.Ren J, Shen S, Wang D, Xi Z, Guo L, Pang Z, Qian Y, Sun X, Jiang X.

The targeted delivery of anticancer drugs to brain glioma by PEGylated oxidized multi-walledcarbon nanotubes modified with angiopep-2. Biomaterials. 2012 Apr;33(11):3324-33.

60.Ding M, Zhang E, He R, Wang X, Li R, Wang W, Yi Q

The radiation dose-regulated AND gate genetic circuit, a novel targeted and real-time monitoringstrategy for cancer gene therapy.

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63.Du Z, Yang Y, Hu Y, Sun Y, Zhang S, Peng W, Zhong Y, Huang X, Kong W.

A long-term high-fat diet increases oxidative stress, mitochondrial damage and apoptosis in theinner ear of D-galactose-induced aging rats.

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64.Wang L, Zhang H, Chen B, Xia G, Wang S, Cheng J, Shao Z, Gao C, Bao W, Tian L, Ren Y, Xu P, Cai X, Liu R, Wang X.

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells.

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65.Yu Y, Liu M, Zhang L, Cao Q, Zhang P, Jiang H, Zou Y, Ge J.

Heat shock transcription factor 1 inhibits H?O?induced cardiomyocyte death through suppression of high-mobility group box 1.

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66.Chang H, Zhang C, Cao Y

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70.Meng X, Li F, Chen S, Tang C, Zhang W, Wang Z, Zhao S

Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats.

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77.Zhang W, Huang Q, Hua Z.

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78.Liu L, Wang P, Liu X, He D, Liang C, Yu Y.

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Macrophages are involved in the protective role of human umbilical cord-derived stromal cells in renal ischemia-reperfusion injury.

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80.Hu X, Li H, Luo S, Liu T, Jiang Y, Liu S.

Thiol and pH dual-responsive dynamic covalent shell cross-linked micelles for triggered release of chemotherapeutic drugs.

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81.Wu YS, Lu HL, Huang X, Liu DH, Meng XM, Guo X, Kim YC, Xu WX.

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82.Yang F, Sun X, Shen J, Yu LP, Liang JY, Zheng HQ, Wu ZD.

A recombined protein (rSj16) derived from Schistosoma japonicum induces cell cycle arrest and apoptosis ofmurine myeloid leukemia cells.

Parasitol Res. 2013 Mar;112(3):1261-72. doi: 10.1007/s00436-012-3260-8. Epub 2013 Jan 15.

83.Xie Q, Xinyong G, Xianjin C, Yayu W.

PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-steptransfection process.

Cytotechnology. 2013 Mar;65(2):263-71. doi: 10.1007/s10616-012-9483-9. Epub 2012 Jul 11.

84.Li H, Zhang X, Zeng S, Chen L, Li X, Lin C, Zhang M, Shu S, Xie S, He Y, Yang L, Tang S, Fu X.

The cellular localization of Na+/H+ exchanger 1, cystic fibrosis transmembrane conductance regulator, potassium channel, e pithelial sodium channel γ and vacuolar-type H+-ATPase in human eccrine sweat glands.

Acta Histochem. 2014 Jul 28. pii: S0065-1281(14)00185-8. doi: 10.1016/j.acthis.2014.07.005.

85.Su J, Hu G, Wang Y, Liang D, Gao M, Sun H, Zhang Y.

Recombinant human growth differentiation factor-9 improves oocyte reprogramming competence and subsequent development of bovine cloned embryos.

Cell Reprogram. 2014 Aug;16(4):281-9. doi: 10.1089/cell.2014.0001. Epub 2014 May 19.

86.Fu Y, Zhang P, Ge J, Cheng J, Dong W, Yuan H, Du Y, Yang M, Sun R, Jiang H.

Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells by inhibiting histone H3K9 acetylation and RUNX2 activity. Int J Biochem Cell Biol. 2014 Sep;54:68-77. doi: 10.1016/j.biocel.2014.07.003. Epub 2014 Jul 11.

87.Chen Z, Zhu M, Pan X, Zhu Y, Yan H, Jiang T, Shen Y, Dong X, Zheng N, Lu J, Ying S, Shen Y.

Inhibition of Hepatitis B virus replication by SAMHD1.

Biochem Biophys Res Commun. 2014 Aug 8;450(4):1462-8. doi: 10.1016/j.bbrc.2014.07.023. Epub 2014 Jul 11.

88.Li D, Zhu B, Ding L, Lu W, Xu G, Wu J.

Role of the mitochondrial pathway in serum deprivation-induced apoptosis of rat endplate cells.

Biochem Biophys Res Commun. 2014 Sep 26;452(3):354-60. doi: 10.1016/j.bbrc.2014.08.054. Epub 2014 Aug 27.

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Xiao-Xu-Ming decoction preserves mitochondrial integrity and reduces apoptosis after focal cerebral ischemia and reperfusion via the mitochondrial p53 pathway.

J Ethnopharmacol. 2014;151(1):307-16. doi: 10.1016/j.jep.2013.10.042. Epub 2013 Nov 1.

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