生化分析仪光学检测系统设计与优化
2018年 第12期仪表技术与传感器Instrument Technique and Sensor 2018 No.12
基金项目:国家自然科学基金项目(61327002);重庆市教委科
研项目(KJ1729406)
收稿日期:2017-11-10生化分析仪光学检测系统设计与优化
武 新1,3,李光林1,温志渝2,廖金权3
(1.西南大学工程技术学院,重庆 400715;2.重庆大学微系统研究中心,重庆 400044;3.重庆电子工程职业学院,重庆 401331) 摘要:面向多参数生化分析仪的测试需求,基于分光光度法,提出一种新型半自动多参数生化分析仪的光学检测系统三系统采用多个不同波长LED 单色光源和多进一出耦合光纤对光源进行分光,实现多参数快速准确测试;采用固定部件,无任何活动构件,大幅度减少了光学检测系统带来的系统误差,保证整个仪器测量精确度和重复性三文中对光学检测系统的光源驱动电流二耦合光纤对LED 光谱的影响二检测室恒温控制分别进行测试,测试数据表明各项光学参数满足分光光度法的要求三对氰化物标准样品基于朗伯比尔定律建标,分析线性度和相关度,对比测试分析仪器的精确度二重复性和最低检出限三各项测试结果均达到设计要求,可实现生化多参数的快速准确测量三
关键词:光谱分析;光学系统;光纤;LED 光源
中图分类号:TN202 文献标识码:A 文章编号:1002-1841(2018)12-0038-04
Design and Optimization of Optical Detection System of Biochemical Analyzer
WU Xin 1,3,LI Guang-lin 1,WEN Zhi-yu 2,LIAO Jin-quan 3(1.College of Engineering and Technology ,Southwest University ,Chongqing 400715,China ;2.Micro System Research Center ,Chongqing University ,Chongqing 400044,China ;
3.Chongqing College of Electronic Engineering ,Chongqing 401331,China )
Abstract :A novel optical detection system of semi-automatic multi-parameter biochemical analyzer was proposed based on spectrophotometry and can meet the needs of multi-parameter testing.The novel system included many LED sources of different wavelength,a coupling fiber of many inputs and one output.It all used fixing part and had not the moving part,which greatly de-creased the system error that the traditional optical detection system brought,and guaranteed the accuracy and repeatability of measurement.This work tested the light source driving current ,the influence of LED spectrum by a coupling fiber,temperature control respectively,the testing data indicated each optical parameter according with the spectrophotometry.The semi-automatic multi-parameter biochemical analyzer with the new system built a calibration curve of cyanide,analyzed the linearity and correla-tion,and calculated the accuracy,repeatability and LOD (limit of detection).Test results of the novel optical detection system
meet the design requirements,the analyzer can measure the biochemical multi-parameters fast and accurately.Keywords :spectrum analysis;optical systems;fiber optics;LED sources 0 引言
基于分光光度法的多参数半自动生化分析仪具
有便携二快速二低成本和智能化的测量特点,可进行生
化多参数浓度测量,已经成为生化参数测量行业应用
最为广泛的仪器之一三半自动多参数生化分析仪的
测量原理是基于分光光度法,在检测室中经过显色反
应后,利用朗伯比尔定律获得被检测物质的浓度[1]三
其典型的组成部分有:光学检测系统二控制系统和数据处理系统三其中光学检测系统[2]由光源系统二光路检测室系统二光电接收系统3部分构成,光学检测系统是半自动生化分析仪快速准确检测的前提三另外,光学检测系统决定着分析仪器的发展趋势:小型化二自动化二精确化二多参数化三通过对国内外研究现状的分析,发现半自动多参数生化分析仪为实现多参数检测其光学检测系统通常采用方案为:(1)对光源进行分光(滤光片)三如国内的AC880型半自动生化分析仪[3]采用卤素灯作为光源,对光源