多发性骨髓瘤患者骨髓间充质干细胞体外生长特性和转化生长因子-β

多发性骨髓瘤患者骨髓间充质干细胞体外生长特性和转化生长因子-β1基因的表达及其意义

雷艳花;陆益龙;朱伟;雷芳;余先球;费小明;朱彦

【期刊名称】《白血病·淋巴瘤》

【年(卷),期】2013(022)004

【摘要】Objective To investigate the expression and significance of transforming growth factor-β1 (TGF-β1) gene in bone marrow mesenchymal stem cell (BMMSC) derived from patients with multiple myeloma (MM).Methods BMMSC of 7 MM patients and 10 patients with iron deficiency anemia were cultured in vitro.The morphology of BMMSC was observed and the growth curve was portrayed according to the daily results of BMMSC proliferation.Total RNA was extracted from BMMSCs and transcription of TGF-β1 gene in BMMSC was measured by reverse transcription-PCR.Results The proliferative activity of BMMSC was not significantly different between the two groups,but expression of TGF-β1 gene of BMMSC was higher in MM patients (0.01241±0.00419) than the control group (0.00122±0.00030) (t =3.218,P ＜0.05).Conclusion The abnormally high expression of TGF-β31 gene in BMMSCs could contribute to the pathogenesis of MM.%目的探讨多发性骨髓瘤(MM)患者骨髓间充质干细胞(BMMSC)体外生长特性和转化生长因子-β 1 (TGF-β 1)基因表达变化及意义.方法以7例MM患者为试验组(MM组),10例单纯缺铁性贫血患者为对照组,取骨髓单个核细胞进行