标记TaqMan探针的常见荧光基团

报告基团英文名称中文名称Em max Abs max M.W颜色FAM6-carboxy-fluorescein6-羧基荧光素495nm521nm537.46Green

TET Tetrachlorofluore

scein

四氯－6－羧基荧

光素

521nm536nm675.24Orange

JOE JOE 2，7－二甲基－

4，5－二氯－6－

羧基荧光素

520nm548nm667Pink

HEX Hexachlorofluorescei

n

六氯－6－甲基荧

光素

535nm556nm744.13Pink

CY3CY3CY3550nm570nm507.59Red

TAMRA Carboxytetramethylrh

odamine

6-羧基四甲基若丹

明

555nm576nm527.53Rose

ROX Carboxy-X-

Rhodamine ROX575nm602nm516.7Red

Texas Red Texas Red Texas Red589nm610nm702Red LC RED640LC RED640LC RED640625nm640nm758Red CY5CY5CY5649nm670nm533.63Violet LC RED705LC RED705LC RED705689nm705nm758Violet

标记TaqMan探针的常见荧光基团