Ndrg2蛋白在匹罗卡品诱导癫痫大鼠模型的表达规律

Ndrg2蛋白在匹罗卡品诱导癫痫大鼠模型的表达规律

陈刚;邓艳春

【期刊名称】《中华神经医学杂志》

【年(卷),期】2011(010)001

【摘要】Objective To test a hypothesis that the protein expression of N-Myc downstream regulated gene 2 (Ndrg2) may be enhanced in the hippocampus of rat models of pilocarpine-induced epilepsy and explore the pattern of Ndrg2 expression. Methods Twenty-eight rats were recruited and randomly divided into 2 groups: 24 rats were enrolled in experimental group while the others in controls.The rat models in the experimental group were induced by lithium-pilocarpine protocol. Then survival rats were sacrificed 4 and 24 h, and 7 d and 6 weeks after the onset of status epilepticus (SE) while the controls were sacrificed 24 h after vehicle treatment. Temporal and spatial protein expression characteristics of Ndrg2 in the hippocampus were analyzed by immunofiuorescence labeling. Results The protein level of Ndrg2 increased by 62% and 37% on the 7th d and in the 6th weeks of onset of SE,respectively; statistically significant differences were noted between the experimental group at the above time points and the control group (P＜0.05). Ndrg2 immunoreactive cells were concentrated along the subgranular zone of the hippocampus. Conclusion The protein expression of Ndrg2 increases significantly in the hippocampus