Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity

Estimation of total phenolic content, in-vitro

antioxidant and anti-inflammatory activity of

flowers of Moringa oleifera

Fatma Alhakmani;Sokindra Kumar;Shah Alam Khan

【期刊名称】《亚太热带生物医学杂志（英文版）》

【年(卷),期】2013(000)008

【摘要】Objective: To evaluate and compare the antioxidant potential and anti-inflammatory activity of ethanolic extract of flowers of Moringa oleifera (M. oleifera) grown in Oman.
Methods: Flowers of M. oleifera were collected in the month of December 2012 and identified by a botanist. Alcoholic extract of the dry pulverized flowers of M. oleifera were obtained by cold maceration method. The ethanolic flower extract was subjected to preliminary phytochemical screening as the reported methods. Folin-Ciocalteu reagent was used to estimate total phenolic content. DPPH was used to determine in-vitro antioxidant activity and anti-inflammatory activity of flowers was investigated by protein denaturation method.
Results:Phytochemical analysis of extract showed presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycosides etc. M. oleifera flowers were found to contain 19.31 mg/g of gallic acid equivalent of total phenolics in dry extract but exhibited moderate antioxidant activity. The anti-inflammatory activity of plant extract was significant and