Cloning, Characterization and Primary Function Study of a Novel Gene, Cymgl, Related to Fa

Cloning, Characterization and Primary Function Study of a Novel Gene, Cymgl, Related to Family 2

Cystatins

Yang XIANG;Dong-Song NIE;Jian WANG;Xiao-Jun TAN;Yun DENG;Shu-Wei LUO;Guang-Xiu LU

【期刊名称】《生物化学与生物物理学报：英文版》

【年(卷),期】2005(037)001

【摘要】Cystatins are cysteine proteinase inhibitors. We found two expression sequence tags (ESTs),CA463109 and AV042522, from a mouse testis library using Digital differential display (DDD). By electrical hybridization, a novel gene, Cymgl (GenBank accession No. AY600990), which has a full length of 0.78 kb,and contains four exons and three introns, was cloned from a mouse testis cDNA library. The gene is located in the 2G3 area of chromosome 2. The full cDNA encompasses the entire open reading frame, encoding 141amino acid residues. The protein has a cysteine protease inhibitor domain that is related to the family 2cystatins but lacks critical consensus sites important for cysteine protease inhibition. These characteristics are seen in the CRES subfamily, which are related to the family 2 cystatins and are expressed specifically in the male reproductive tract. CYMG1 has a 44% (48/108) identity with mouse CRES and 30% (42/140)identity with mouse cystatin C. Northern blot analysis showed that the Cymgl is specifically expressed in adult