AMG_517_LCMS_08471_MedChemExpress

=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 49

Acq. Instrument : HY-LCMS-02 Location : Vial 37Injection Date : 3/19/2015 1:00:22 PM Inj : 1

Inj Volume : 3.000 μl

Acq. Method : D:\AGLIENT 1260\DATA\20150319\20150319 2015-03-19 09-00-09\100-1000MS+3MIN- 1.5_(0.02%FA).M

Last changed : 3/19/2015 9:00:09 AM by Li Shan(LCMS-02)

Analysis Method : D:\AGLIENT 1260\METHOD\HY-303_17-H01RS(LCMS).M Last changed : 3/19/2015 3:01:44 PM by Li Shan(LCMS-02) (modified after loading)Method Info : HPLC

Catalog No : HY-10634 Batch#08471 A-RP-132

Additional Info : Peak(s) manually integrated

min

0.5

1

1.52

2.53mAU 0

200400600800100012001400 DAD1 B, Sig=214,4 Ref=off (D:\AGLIENT...0\DATA\20150319\20150319 2015-03-19 09-00-09\CPK2015-319-08471.D)

1.539

2.231

2.324

2.654

===================================================================== Area Percent Report =====================================================================

Sorted By : Signal Multiplier : 1.0000Dilution : 1.0000

Do not use Multiplier & Dilution Factor with ISTDs

Signal 1: DAD1 B, Sig=214,4 Ref=off

Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %

----|-------|----|-------|----------|----------|--------| 1 1.539 MM 0.0469 7.95452 2.82567 0.1970 2 2.231 MM 0.0341 2.22033 1.08644 0.0550 3 2.324 MM 0.0431 4016.43384 1553.01147 99.4617 4 2.654 MM 0.1081 11.56137 1.45189 0.2863

Totals : 4038.17006 1558.37548

===================================================================== *** End of Report ***

=====================================================================Acq. Operator : Li Shan(LCMS-02) Seq. Line : 49

Acq. Instrument : HY-LCMS-02 Location : Vial 37Injection Date : 3/19/2015 1:00:22 PM Inj : 1

Inj Volume : 3.000 μl

Acq. Method : D:\AGLIENT 1260\DATA\20150319\20150319 2015-03-19 09-00-09\100-1000MS+3MIN- 1.5_(0.02%FA).M

Last changed : 3/19/2015 9:00:09 AM by Li Shan(LCMS-02)

Analysis Method : D:\AGLIENT 1260\METHOD\HY-303_17-H01RS(LCMS).M Last changed : 3/19/2015 3:02:57 PM by Li Shan(LCMS-02) (modified after loading)Method Info : HPLC

Catalog No : HY-10634 Batch#08471 A-RP-132

Additional Info : Peak(s) manually integrated

min

0.5

1

1.5

2

2.5

3

50000

100000150000200000250000 MSD1 TIC, MS File (D:\AGLIENT 1260\DATA\20150319\20150319 2015-03-19 09-00-09\CPK2015-319-08471.D) ES-API, Pos, Sc

2.327

MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 50 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts.

Reportable Ion Abundance: > 10%.

Retention Mol. Weight Time (MS) MS Area or Ion

2.327 1457918 431.70 I 430.70 I

m/z

100

150

200

250

300

350

400

450

20406080100*MSD1 SPC, time=2.309:2.345 of D:\AGLIENT 1260\DATA\20150319\20150319 2015-03-19 09-00-09\CPK2015-319-08471.D ES-API Max: 169451 432.8 431.7

430.7

*** End of Report ***