pBI121质粒图谱及载体信息
pBI121质粒图谱及载体信息
启动子：35S
终止子：Nos
报告基因：GUS
抗性基因：NptII
pBI121载体信息：
LOCUS AY781296 8196 bp DNA linear SYN 10-NOV-2004 DEFINITION Expression vector pBI121_pro T-DNA region, complete sequence. ACCESSION AY781296
VERSION AY781296.1 GI:55418080
KEYWORDS .
SOURCE Expression vector pBI121_pro
ORGANISM Expression vector pBI121_pro
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.
TITLE Construction of the plant expression vector of the proBA gene from
Bacillus subtilis mutant to proline analog and analysis of the salt
tolerance of transgenic Arabidopsis thaliana
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8196)
AUTHORS Zeng,Y.H., Cao,J.W., Cai,H.Y., Chen,X.H. and Miao,L.X.
TITLE Direct Submission
JOURNAL Submitted (14-OCT-2004) College of Life Science, Wuhan University, Bayi Road, Wuhan, Hubei 430072, P.R.China
FEATURES Location/Qualifiers
source 1..8196
/organism="Expression vector pBI121_pro"
/mol_type="other DNA"
/db_xref="taxon:299464"
misc_feature 1..306
/note="contains T-DNA right border and Nos promoter"
gene 318..1112
/gene="NptII"
CDS 318..1112
/gene="NptII"
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase II"
/protein_id="AA V51261.1"
/db_xref="GI:55418081"
/translation="MIEQDGLHAGSPAAWVERLFGYDW AQQTIGCSDAA VFRLS AQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAA VLDVVTEAGRDWL LLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAG LVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDC GRLGV ADRY
QDIALATRDIAEELGGEW ADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 1113..1365
/note="Nos"
promoter 2059..2859
/note="CaMV 35S"
misc_feature 2872..3174
/note="Arabidopsis thaliana p5csB intron in GenBank
Accession Number X86778"
gene 3175..4287
/gene="proB"
CDS 3175..4287
/gene="proB"
/codon_start=1
/transl_table=11
/product="gamma-glutamyl kinase"
/protein_id="AA V51262.1"
/db_xref="GI:55418082"
/translation="MKKQRIVVKIGSSSLTNSNGSIDEAKIKEHTEAISLLKENGHE V
ILITSGA V AAGFSALGYPARPVTIKGKQAAAA VGQTLLMQQYMDHLKRY GLTPAQILL
TRNDFSKRERYRNAYATVMELLERGVVPIINENDSTSVEELTFGDNDMLS ALVSGLIH
ADQLMILTDINGL YDANPNENPDAKRFDYLTDITPELLGYAGSAGSKVGT GGMKSKFL
AAQTALSLGVKVFIGTGAGREKLKVILDGKGDGTYIGDKELSTVNNTRQ WIMFHSPIS
GEIIIDGGAEQAMIHNGSSLLPAGV ADVCGSFPKGAVVEVRGPGGIIGKGQ THYSSDE
ILEAKGKRSDQLPGAKQVEVIHRNDWVNLFDEGENK"
misc_feature 4300..4602
/note="Arabidopsis thaliana p5csB intron in GenBank
Accession Number X86778"
gene 4603..5733
/gene="proA"
CDS 4603..5733
/gene="proA"
/codon_start=1
/transl_table=11
/product="glu-5-semialdehyde"
/protein_id="AA V51263.1"
/db_xref="GI:55418083"
/translation="MSEVLQKAALAKEAAAEMVMKTTAEKNEALQCIADGLRN ERQLI
LTENQKDIEAGQKRGLTPDIIDRLTLDEKRLLDIADA VELLIGLEDPVGESL ETIQKE
NGLSIEKIRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVVLRGSSSAIH SNKALVSV
MKRALGLSKLPIDA VQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDLV VRESTVP
VLETGAGNCHVYIDESADPDMARDVVINAKTQRPSVCNAIESLLIHEKW AEVHGRKLL
NQLTEKGVELRGDQMICELEPNAKQAEEADWGAEYLAPILSVKTVHDV QEA VRHIQQY
GTNHSEAILTENGEHAAYFQTA VDAAA VYHNASTRFTDGFEF"
gene 5758..7569
/gene="GUS"
CDS 5758..7569
/gene="GUS"
/codon_start=1
/transl_table=11
/product="beta-glucuronidase"
/protein_id="AA V51264.1"
/db_xref="GI:55418084"
/translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQE SRA
IA VPGSFNDQFADADIRNY AGNVWYQREVFIPKGWAGQRIVLRFDA VTH YGKVWVNNQ
EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITD ENGKKKQSY
FHDFFNYAGIHRSVML YTTPNTWVDDITVVTHV AQDCNHASVDWQVV A NGDVSVELRD
ADQQVV ATGQGTSGTLQVVNPHLWQPGEGYL YELCVTAKSQTECDIYPL RVGIRSV A V
KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGA NSYRTSHYPYA
EEMLDWADEHGIVVIDETAA VGFNLSLGIGFEAGNKPKELYSEEA VNGET QQAHLQAI
KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT CVNVMFCD
AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQ PIIITEYGVD
TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSA VVGEQVWNFADFA TSQGILRVGGN
KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
terminator 7630..7882
/note="Nos"
misc_feature 7883..8196
/note="T-DNA left border"
ORIGIN
1 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta
61 agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact
121 gacagaaccg caacgattga aggagccact cagccgcggg tttctggagt ttaatgagct
181 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
241 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
301 aattaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
361 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 421 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
481 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 541 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
601 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 661 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 721 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 781 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 841 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 901 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
961 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 1021 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
1081 ccttctatcg ccttcttgac gagttcttct gagatcgttc aaacatttgg caataaagtt
1141 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
1201 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
1261 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
1321 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggcc tcctgtcaat
1381 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 1441 ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 1501 gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
1561 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
1621 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
1681 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
1741 ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
1801 tttgtctttg gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
1861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1921 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
1981 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
2041 aagcttgcat gcctgcagcc cacagatggt tagagaggct tacgcagcag gtctcatcaa 2101 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga 2161 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
2221 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt
2281 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa
2341 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag 2401 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
2461 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
2521 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
2581 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 2641 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac 2701 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 2761 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
2821 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactctag agtttgaaat
2881 tctctgtgaa ttttttttgt tgattgatga ttgtcttcaa aaacattcct gggtttgact
2941 tttttttcat cggagcttcg tctgtgttgt tggttgatgt caattggtat ttgtattaat
3001 ccgtactttt tcaatgattg ctttcttaag ttttttaaca tttgtttttt ggcggcggag
3061 aaatctggag taatgttgaa aaacaatgac aattttgttt ctttcaatcc taaattttcg
3121 atttttctca ctacccttat ctctatttga tcgatttttt gttattgttt acagatgaaa
3181 aagcagagaa ttgttgtgaa aatcgggagc agctcgctca caaacagtaa cggaagtatt 3241 gatgaagcca aaatcaagga gcatacggaa gccatttcat tattaaaaga aaacgggcat
3301 gaagtaatcc tcattacctc aggcgcggtg gcagccggtt tttccgctct cggctatccg
3361 gcgcgccccg ttacgataaa aggaaagcag gcagccgccg cggtcggcca gacgctttta 3421 atgcagcaat atatggacca tttaaaaaga tacggtctga cgccggcgca aattttatta
3481 acaagaaatg atttctcaaa aagagagcgg tacagaaatg cgtatgccac ggtaatggaa 3541 ttattggagc ggggagtcgt gccgattatt aatgagaatg attccacatc agtcgaagaa
3601 ctgacgttcg gagataatga catgctgtcg gcgcttgtga gcggcctgat tcatgcagac
3661 caattaatga ttctcacaga cattaacggg ctgtacgatg ccaatccgaa tgaaaaccct
3721 gacgcaaagc ggtttgacta tttgactgac attacacctg aattgctcgg gtatgccgga
3781 tcagcgggct cgaaggtcgg caccggaggc atgaagtcaa aatttctcgc tgcccagacc 3841 gcattgtcgc tcggagttaa ggtgtttatc ggaaccggtg ccggacggga aaaactgaag 3901 gtgattttag acggtaaagg cgacggcaca tatatcggtg ataaagagct gtccaccgtc
3961 aataatacac ggcaatggat catgtttcat tctccgatat caggggaaat catcattgat
4021 gggggagcgg agcaggcgat gatccataac ggctcaagtc tccttccggc cggtgtcgcc 4081 gatgtctgcg gcagttttcc gaaaggggcc gtggtggaag tcagaggtcc cggcggcatc 4141 atcggcaaag ggcagacgca ttactcatcc gatgagattt tggaagccaa agggaagcgg 4201 agcgatcagc ttccgggcgc gaagcaggtg gaagtgattc acagaaatga ttgggtgaat 4261 ctatttgacg agggggaaaa caaatgatct agaggatccg tttgaaattc tctgtgaatt
4321 ttttttgttg attgatgatt gtcttcaaaa acattcctgg gtttgacttt tttttcatcg
4381 gagcttcgtc tgtgttgttg gttgatgtca attggtattt gtattaatcc gtactttttc
4441 aatgattgct ttcttaagtt ttttaacatt tgttttttgg cggcggagaa atctggagta
4501 atgttgaaaa acaatgacaa ttttgtttct ttcaatccta aattttcgat ttttctcact
4561 acccttatct ctatttgatc gattttttgt tattgtttac agatgagtga agtattgcaa
4621 aaagctgcct tggcaaaaga agcggctgcc gaaatggtca tgaaaacgac tgctgaaaaa 4681 aacgaagcgc tccaatgtat tgcagacgga ctcagaaatg aacgccagct gattttaaca
4741 gagaatcaga aagatattga agcgggacag aaaagagggc tgacacctga cattattgac 4801 aggctgactc ttgatgaaaa acgcctgctc gatatcgcgg atgctgtaga actgttaatc
4861 ggtctggaag atcccgtcgg tgaatcattg gagaccattc aaaaagagaa tggattatcc
4921 attgaaaaaa tccgcgtgcc cctcggagta gtgggcatga tctatgaagc cagaccgaat 4981 gttaccgtag acgctgccac actttgcctg aaaaccggaa acgcggttgt gctcagagga 5041 agttcgtcag ccatccacag caacaaagcg cttgtcagtg tgatgaaacg cgcactggga 5101 ctgtcaaagc ttccgattga tgccgttcag ctcatagaag atacaagcaa agaaaccgcg
5161 aaacagctct tcacattaaa tgacgggctg gatgtattga tcccgcgcgg cggcaaaaat
5221 ttgattgatc tggtggtcag ggaatcaacc gtacccgtgt tagaaaccgg agcgggcaac 5281 tgccacgtgt acatagatga gtcagcagat cccgatatgg caagagatgt cgtcatcaac
5341 gccaaaacac agcggccttc tgtctgcaat gcgattgaat ctcttttgat tcatgaaaaa
5401 tgggcggaag tacacggacg aaagctgctg aatcagctga cagaaaaagg ggtggagcta 5461 cgcggagatc agatgatttg cgagctggag cctaatgcga aacaagccga agaggccgat
5521 tggggagcgg aatacctggc gccgattctc agtgtaaaaa cggtgcatga cgtacaggag 5581 gcggtccgcc acattcagca gtacggcaca aaccattcag aagccattct gacggaaaac 5641 ggtgagcatg ccgcttactt ccagacagcc gttgacgccg ccgccgtgta tcataatgct 5701 tccacacggt ttacagacgg ctttgaattc tgaggatccc cgggtggtca gtcccttatg
5761 ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc 5821 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa 5881 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt
5941 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc
6001 cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat
6061 caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat 6121 gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag
6181 actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc 6241 catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac
6301 acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct 6361 gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa 6421 caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc 6481 tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag 6541 tgtgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc 6601 ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg
6661 cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt
6721 ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca 6781 gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta
6841 ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac 6901 ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac 6961 cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca 7021 cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc 7081 acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat
7141 gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca 7201 gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc 7261 atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg 7321 agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc
7381 gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg
7441 cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct 7501 tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc 7561 aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg ctacagcctc gggaattgct
7621 accgagctcg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
7681 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
7741 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
7801 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg 7861 gtgtcatcta tgttactaga tggaattcac tggccgtcgt tttacaacgt cgtgactggg
7921 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
7981 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 8041 aatgaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
8101 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
8161 gcacaaaatc accactcgat acaggcagcc catcag