常用限制性内切酶的识别序列和同裂酶

酶同裂酶识别序列酶同裂酶识别序列酶ACC I-GT (A/C)(G/T)AC BsuR I Hae III GG CC Msp I4 ACC III BspE II T CCGGA Cfo I Hha I GCG C

Bspm II HinP1 I G CGC Mst II

Mro I Cfr9 I Xma I C CCGGG Nar I Aha II Acy I G(A/G) CG(T/C)C Sma I CCC GGG

Hsp92 I Cfr13 I Asu I G GNCC

Aha III Dra I TTT AAA Sau96 I

Alu I-AG CT Cfr42 I Sac II CCGC GG Nco I Alw26 I2BsmA I GTCTC(1/5)Cla I Ban III AT CGAT Nde I Alw44 I ApaL I G TGCAC Bsp106 I Nde II SnoI BspD I

Apa I-GGGCC C Bsu15 I Nhe I Asp I Tth111 I GACN NNGTC Csp I Rsr II CG G(A/T)CCG

Asp718 I Acc65 I G GTACC Cvn I Bsu36 I CC TNAGG Not I Kpn I GGTAC C Dde I- C TNAG Nst I Asn I Vsp I AT TAAT Dpn I3-GmeA TC

Asu I Sau96 I G GNCC Dra I Aha III TTT AAA

Cfr13 I Ecl136 II EcolCR I GAG CTC Nsp III Asu II Csp45 I TT CGAA Sal I GAGCT C PaeR7 I Ava I Eco88 I C(T/C)CG(A/G)G Eco24 I Ban II G(AG)GC(TC) C Pal I Nsp III Eco32 I EcoR V GAT ATC Pst I Ava II Sin I G G(A/T)CC Eco64 I Ban I G G(TC)(AG)CC Pvu I Eco47 I Eco88 I Ava I C (TC)CG(AG)G Pvu II Axy I Bsu36 I CC TNAGG Eco91 I BstE II G GTNACC Rsa I Bal I Msc I TGG CCA Eco147 I Stu I AGG CCT Rsr II MluN I Eco255 I Sca I AGT ACT Sac I BamH I-G GATCC EcolCR I Ecl136 II GAG CTC

Ban I BshN I G G(T/C)(A/G)CC Sac I

Eco64 I Sst I GAGCT C Sac II

HgaC I EcoR I-G AATTC

Ban III Cla I AT CGAT EcoR II BstO I CC (A/T)GG

Bbe I-GGCGC C EcoR V Eco32 I GAT ATC Sal I Nar I GG CGCC EcoT14 I Sty I C C(A/T)(A/T)GG Sau I Bbu I Pae I GCATA C EcoT22 I Nsi I ATGCA T Sau3A I Sph I Eco47 I Ava II G G(A/T)CC

Bcl I-T GATCA Sin I Sau96 I Bcn I Nci I CC (C/G)GG Eco81 I Bsu36 I CC TNAGG

Bst98 I C TTAAGG Fok I-GGATG(9/13)Sca I Bgl I-GCCNNNN NGGC Hae II Bsp143 II(A/G)GCGC (T/C)Sfi I BseN I BsrS I ACTGGN Hae III BsuR I GG CC Sin I Bsr I Pal I

Bgl II- A GATCT Hap II Hpa II C CGG Sma I BshN I Ban I G G(T/C)(A/G)CC Msp I

Bsp19 I Nco I C CATGG HgaC I Ban I G G(T/C)(A/G)CC

Bsp68 I Nru I TCG CGA Hha I Cfo I GCG C Spe I Bsp106 I Cla I AT CGAT HinP1 I G CGC Sph I

Bsp143 I Mbo I GATC Hinc II Hind II GT(T/C) (A/G)AC

Bsp143 II Hae II(A/G)GCGC (T/C)Hind III- A AGCTT Ssp I BspD I Cla I AT CGAT Hinf I-G ANTC Sst I BspE I Acc III T CGGA HinP1 I-G CGC

BspM II Acc III T CCGGA Hha I Cfo I GCG C Sst II Bst71 I2Bbv I GCAGC(8/12)Hpa I-GTT AAC Sty I Bst98 I Afl II C TTAAG Hpa II Msp I C CGG Taq I Bfr I Hap II Tru9 I BstB I Csp45 I TT CGAA Kpn I-G GTACC Tru II BstE II BstP I G GTNACC Asp718 I GGTAC C Tth111 I Eco91 I Acc65 I

BstN I BstO I CC (A/T)GG Ksp I Sac II CCGC GG TtHB8 I Bst0 I Apy I CC (A/T)GG Xba I BstN I Mbo I Sau3A I GATC Xho I

EcoR II Nde II Xho II

Mva I Bsp143 I

BstX I-CCANNNNN NTGG Mbo II-GAAGA(8/7)Xma I BstY I Xho II(A/G) GATC(T/C)Mfl I Xho II(A/G) GATC(T/C)

Bsu15 I Cla I AT GAT Mlu I- A CGCGT Xma III Bsu36 I Axy I CC TNAGG MluN I Bal I TGG CCA

Sau I Mro I Acc III T CCGGA

MstII Msc I Bal I TGG CCA

Cvn I

Aoc I

Eco81 I

同裂酶识别序列

Hpa II C CGG

Hap II

Bsu36 I CC TNAGG

-GG CGCC Ehe I GGC GCC

Kas I G GCGCC

Bbe I GGCGC C

Bsp19 I C CATGG

-CA TATG Mbo I GATC

Sau3A I

-G CTAGC Sph I

-GC GGCCGC Ava III ATGCA T EcoT22 I

Mph1103 I

Ava I C (T/C)CG(A/G)G Xba I T CTAGA Hae III GG CC

-CTGCA G

Xor II CGAT CG

-CAG CTG

-GT AC Csp I CG G(A/T)CCG

Sst I GAGCT C

Ecl136 II GAG CTC EcolCR I GAG CTC

Sst II CCGC GG

Ksp I

Cfr42 I

-G TCGAC

Bsu36 I C CTNAGG

Mbo I GATC

Nde II

Asu I G GNCC

Cfr13 I

Eco255 I AGT ACT

-GGCCNNNNN NGGCC Ava II G G(A/T)CC Eco47 I

-CCC GGG Xma I C CCGGG

Cfr9 I

- A CTAGT Bbu I GCATG C

Pae I

-AAT ATT

Sac I GAGCT C EcolCR I GAG CTC

Sca II CCGC GG EcoT14 I C C(A/T)(A/T)GG TthHB8 I T CGA

Mse I T TAA

Tth111 I GACN NNGTC Asp I GACN NNNGTC Tru II

Taq I T CGA

-T CTAGA PaeR7 I C TCGAG BstY I(A/G) GATC(T/C) Mfl I

Cfr I C CCGGG Sma I CCC GGG

Eco52 I C GGCCG BstZ I

Eag I

EclX I