PCR引物设计

PCR引物设计

ORIGIN

1 atgcgtaact ttgatttatc cccgctttac cgttctgcta ttggatttga ccgtttgttt

61 aaccacttag aaaacaacca gagccagagt aatggcggct accctccgta taacgttgaa

121 ctggtagacg aaaaccatta ccgcattgct atcgctgtgg ctggttttgc tgagagcgaa

181 ctggaaatta ccgcccagga taatctgctg gtggtgaaag gtgctcatgc cgacgaacaa

241 aaagagcgca cctatctgta ccagggcatc gctgaacgca actttgaacg caaattccag

301 ttagctgaga acattcatgt tcgtggtgct aacctggtaa atggtttgct gtatatcgat

361 ctcgaacgcg tgattccgga agcgaaaaaa ccgcgccgta tcgaaatcaa ctaa

Non Cut Enzymes

AatII Acc65I AcyI AflII AgeI AhaIII

Alw26I Alw44I AlwNI ApaBI ApaI ApaLI

AscI Asp718I AsuI AsuII AvaI AvaII

AvrII BalI BamHI BanI BanII BbeI

BbvI BbvII BclI BglI BglII Bpu1102I

BsaHI BsaOI Bsc91I BsiI BsmI Bsp1407I

BspHI BspMI BssHII Bst71I BstD102I BstEII

Bsu36I Cfr10I CfrI Csp45I CspI CvnI

DraI DraII DraIII DrdI EagI Eam1105I

Ecl136II Eco31I Eco47III Eco52I Eco56I Eco57I

Eco72I EcoHI EcoICRI EcoNI EcoRI EcoRV

EheI EspI FokI FseI HaeII HaeIII

HgaI HindII HindIII HpaI I-PpoI KpnI

MaeI MboII MfeI Mlu113I MscI MspA1I

MstI MstII NaeI NarI NcoI NdeI

NheI NlaIV NotI NruI NsiI NspBII

NspI PacI PflMI PinAI PleI PmaCI

PmeI PpuMI PssI PstI PvuI PvuII

RleAI SacI SacII SalI SapI SauI

ScaI SciI SfiI SgrAI SmaI SnaBI

SpeI SphI SplI SpoI SrfI SspI

SstI SstII StuI StyI SunI SwaI

Tth111I Tth111II VspI XbaI XcmI XhoI XhoII XmaI XmaIII XmnI XorII

PET-28a Cut Enzymes

XhoI NotI EagI HindIII SalI SacI EcoRI BamHI NheI NdeI NcoI

Common Cut Enzymes

XhoI NotI EagI HindIII SalI SacI EcoRI BamHI NheI NdeI NcoI

EcoRI :GAATTC

HindIII :AAGCTT

P1:GAATTC atgcgtaact ttgatttatc

P2:AAGCTT ttagttgatt tcgatacggc