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光合细菌利用有机废水连续产氢的试验研究

工业用水与废水

INDUSTRIAL WATER&WASTEWATER Vol.49No.2Apr.,2018

秦芳玲1,王啸熠1,文星1,2,刘文辉3

(1.西安石油大学化学化工学院,西安710065;2.中国石油兰州化工研究中心环境化工所,兰州730060;

3.西安工业大学材料与化工学院,西安710021)

光合细菌制氢是在适宜的光照强度下,利用光

合细菌分解有机质,释放氢气的一种生物制氢技

术三该技术是将光能利用二产氢及有机物去除有机

结合的一种生物制氢方式,具有污染少,能量利用

率高二耗能低的优点,是最具发展潜力的生物制氢

方式之一[1-3]三截止到目前,已经在国际上报道的可以进行生物制氢的微生物可分为5种:真核藻

类二固氮菌二异养型厌氧细菌二厌氧光营养细菌和蓝细菌,这5种分别代表了不同产氢类型[4]三本研究中的厌氧光营养细菌属于球形红螺菌属,能在厌氧光照或好氧光照条件下利用自然界中的有机物二硫化物二氨等作为供氢体兼碳源进行光合作用[5]三在1962年,Gest[6]首次证明球形红螺菌在厌氧光照条件下产生氢气,从而正式开启了微生物光合产氢研究的大门三20世纪七八十年代,能源危机促使光合产氢的研究达到了一个高潮三目前光合生物

摘要:为考察光合细菌连续性生物制氢过程中碳源浓度二水力停留时间等因素对连续运行稳定性能和制氢量的影响,通过改变碳源浓度和水力停留时间来测定产氢量以及产氢率,确定最佳的连续运行条件三结果表明:光合细菌利用含糖废水为基质进行连续性生物制氢过程中,在最佳温度为30?二最佳光照强度为3000Lux的情况下,且当葡萄糖质量浓度为12g/L,水力停留时间为72h时,产氢效率最高,连续产氢速率达45mL/h三关键词:光合细菌;生物制氢;碳源浓度;水力停留时间

中图分类号:X703.1文献标志码:A文章编号:1009-2455(2018)02-0032-06

Experimental study on continuous production of hydrogen with

photosynthetic bacteria from organic wastewater

QIN Fang-ling1,WANG Xiao-yi1,WEN Xing1,2,LIU Wen-hui3

(1.College of Chemistry and Chemical Engineering,Xi'an Shiyou University,Xi'an710065,China;2.Environmental

Chemical Industry Institute of PetroChina Lanzhou Chemical Industry Research Center,Lanzhou730060,China;

3.Faculty of Materials and Chemical Engineering,Xi'an Industrial University,Xi'an710021,China)

Abstract:In order to investigate the effect of carbon source concentration,hydraulic retention time and some other factors on the stability and the hydrogen production capacity of the photosynthetic bacteria continuous hydrogen production system,an experiment was carried out.Through changing the carbon source concentration and the hydraulic retention time to measure hydrogen production capacity and rate,the optimal condition for the system continuous operation was determined.The results of the experiment showed that,for the continuous hy-drogen production by photosynthetic bacteria with sucrose-containing wastewater as the substrate,the optimal condition was as follows:the temperature was30?,the illumination intensity was3000Lux,the mass concen-tration of glucose was12g/L,the hydraulic retention time was72h.Under the above condition,the hydrogen production rate reached the highest and the velocity was as high as45mL/h.

Keywords:photosynthetic bacteria;biological hydrogen production;carbon source concentration;hydraulic retention time

光合细菌利用有机废水连续产氢的试验研究

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