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燃料法荧光定量PCR分析模版

CELF5过表达CELF5过表达UL8325.072124.275924.149726.2775β-actin20.225520.329220.388620.2181ΔCT 4.8466 3.9467 3.7611 6.0594均值ΔCT 4.1848 5.240166667

对照对照

UL8330.445524.360824.927726.422β-actin19.750620.121220.312518.3777ΔCT10.6949 4.2396 4.61528.0443均值ΔCT 6.516566667 6.8577

ΔΔCT-2.331766667-1.617533333 2-ΔΔCT 5.034214423 3.068499468

干扰干扰

UL8324.733124.276323.648124.0403β-actin17.455717.771217.483617.5939ΔCT7.2774 6.5051 6.1645 6.4464均值ΔCT 6.649 6.3439

ΔΔCT0.132433333-0.5138 2-ΔΔCT0.912291428 1.42780603

干扰对照干扰对照

UL8337.044137.975824.0506β-actin30.05730.944517.3818ΔCT 6.98717.0313 6.6688均值ΔCT7.0092 6.735466667ΔΔCT0.492633333-0.122233333 2-ΔΔCT0.710726633 1.088418462

CELF5过表达

26.279825.079724.003324.590424.5996 21.438120.260320.881919.855319.0383 4.8417 4.8194 3.1214 4.7351 5.5613

4.4726

对照

23.494727.115625.472324.610124.0619 19.317218.764318.361418.054317.8639 4.17758.35137.1109 6.5558 6.198

6.621566667

-2.148966667

4.435100101

干扰

24.042524.944224.977225.049824.1739 18.234718.166717.643616.658616.9953 5.8078 6.77757.33368.39127.1786

7.634466667

1.0129

0.495549129

干扰对照

24.034524.029933.836625.547729.2051 17.390617.136222.594422.376827.8587 6.6439 6.893711.2422 3.1709 1.3464

5.253166667

-1.3684

2.58184072

2-ΔΔCT2-ΔΔCT

2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT2-ΔΔCT