Physiological, histopathological and cellular immune effects of Pergularia tomentosa extra

Physiological,histopathological and cellular immune effects of Pergularia tomentosa extract on Locusta migratoria nymphs

Meriam Miladi1＊,Khemais Abdellaoui1＊,Amel Ben Hamouda1,lteb Boughattas1,Mouna Mhafdhi2,Fatma Acheuk3,Monia Ben Halima-Kamel1

【摘要】Abstract The migratory locust Locusta migratoria (Orthoptera,Acrididae) is one of the most important pests due to its extensive and severe damage to crops in large parts of Africa and Asia. Biodegradable and ecologically natural products such as botanical insecticides are emerging candidates for replacement of usually applied chemical pesticides. The crude methanolic extract of Pergularia tomentosa (PME) was investigated for their toxicity and physiological aspects on L. migratoria nymphs. Results showed that treatment of newly emerged fourth and fifth instar nymphs resulted in significant mortality and significant repellent activity with an LC50value of 0.18 and 0.38%,respectively,after seven days of treatment.The PME toxicity was also demonstrated by histopathological changes in the alimentary canal resulting in considerable disorganization and severe damage of the caeca and proventriculus structure. The extract induced cellular immune reactions which manifested by a significant decrease in the number of the differential haemocyte counts (prohemocytes and plasmatocytes)