金银花根围AM真菌多样性的季节性变化研究

金银花根围AM真菌多样性的季节性变化研究

贺学礼;耿晓进;赵丽莉;牛凯

【期刊名称】《生态环境学报》

【年(卷),期】2013(000)001

【摘要】In this paper, the seasonal variation of AM fungal diversity in the rhizosphere of Lonicera japonica was investigated by the methods of nested-PCR and DGGE to explore seasonal variation of AM fungal diversity and the influencing factors. Soil samples were collected in the rhizosphere of L. japonica and divided a depth of 30 cm into 3 sections, i. e. 0-10, 10-20 and 20-30 cm from Anguo city of Hebei province in March, June, September and December 2011 respectively. The results showed that a total of 15 molecular species of AM fungi were discovered. Shannon-Weiner indices, richness and evenness of AM fungi were first decreased and then increased from spring to winter, minimum values occurred in summer. Soil sampling depth had a significant influence on AM fungal diversity. AM fungal diversity had a decreasing trend with soil layers deepen. Cluster analysis showed that AM fungal community composition has significant differences with seasonal change and had similar community composition in winter and spring, and in summer and autumn respectively. Correlation analysis revealed that soil total N was significantly and positively correlated with richness, Shannon-Weiner and Simpson’s diver sity indices. Soil pH was