Cloning and Characterization of a Differentially Expressed Phenylalanine Ammonialyase Gene

Cloning and Characterization of a Differentially Expressed Phenylalanine Ammonialyase Gene (liPAL) After Genome Duplication from Tetraploid

Isatis indigotica Fort.

Bei-Bei Lu; Zhen Du; Ru-Xian Ding; Lei Zhang; Xiao-Jing Yu; Cheng-Hong Liu; Wan-Sheng Chen

【期刊名称】《《植物学报（英文版）》》

【年(卷),期】2006(048)012

【摘要】Phenylpropanoid derivatives are a complex class of secondary metabolites that have many important roles in plants during normal growth and in responses to environmental stress. Phenylalanine ammonialyase(PAL) catalyzes the first step in the biosynthesis of phenylpropanoids. In the present study, we isolated a novel phenylalanine ammonialyase gene (designated as liPAL) from tetraploid Isatis indigotica Fort. by rapid amplification of cDNA ends (RACE), which was a cultivar from the diploid plant by genome duplication.The full-length cDNA of liPAL was 2 530-bp long with an open reading frame (ORF) of 2 178 bp encoding a polypeptide of 725 amino acid residues. Analysis of liPAL genomic DNA revealed that it was structurally similar to other plant PAL genes, with a single intron at a conserved position, and a long highly conserved second exon. Semi-quantitative RT-PCR revealed that the liPAL expression in roots and leaves from a tetraploid