Establishment of a Two-site ELISA for Detection of Chlamydia Trachomatis

Establishment of a Two-site ELISA for Detection of

Chlamydia Trachomatis

WANG Huj(王慧);XIONG Ljkuan(熊礼宽);LI Ying(李影)

【期刊名称】《中华性传播感染杂志（英文版）》

【年(卷),期】2002(002)001

【摘要】Objective:To establish a rapid and simple assay for the diagnosis of Chlamydia trachonatis (CT) infection.Methods BALB/c mice were immunized with SDS-PAGE purified major outer membrane protein (MOMP) from CT and the monoclonal antibodies were obtained subsequently.Two-site ELISA was developed to detect CT infection.Results: The established assay was able to detect as low as 1.248ug/ml MOMP with interrun and inrun CV 6.9% and 3.1% respectively. 94% (34/36) of culture-positive samples were found to be positive in the current examination,indicating the high sensitivity of this assay.Conclusion: The assay is applicable for clinical diagnosis of CT infection.

【总页数】3页(41-43)

【关键词】chlamydia trachomatis;ELISA;major outer membrane protein 【作者】WANG Huj(王慧);XIONG Ljkuan(熊礼宽);LI Ying(李影)

【作者单位】Department of Biology, Xu Zhou Medical College, Xu Zhou, 221002, China;Department of Clinical Laboratory, Institute of Shenzhen Chronic Disease Prevention and Treatment, Shenzhen, 518020, China;Da