电穿孔法转化大肠杆菌TG1的条件优化

电穿孔法转化大肠杆菌TG1的条件优化

李南;凌世淦;吴梧桐

【期刊名称】《药物生物技术》

【年(卷),期】2001(008)003

【摘要】Electroporation was performed to transfer plasmi d DNA of pFab74X into E.coli strain TG1.A number of factors that effected transformation effi ci ency were investigated.High transformation efficiency was obtained with an app ropriat e combination of field strength and pulse time.Transformation efficiency could also be elevated by using hosted cells harvested in the early exponential growth phase, increasing the density of cells or decreasing the concentration of Ampci llin in the course of screening.%研究了质粒pFab74X经电穿孔转化大肠杆菌TG1菌株的影响因素，并优化其转化条件。电场强度和脉冲时间的影响较为复杂，两者适当组合才可获得高转化效率。此外，采用对数生长早期的细菌，提高细菌浓度和降低筛选用氨苄青霉素的浓度均可提高转化效率。

【总页数】4页(143-146)

【关键词】电穿孔;转化效率;大肠杆菌TG1

【作者】李南;凌世淦;吴梧桐

【作者单位】中国药科大学生物制药学院，南京210009;军事医学科学院基础医学研究所分子病毒学实验室，北京100850;中国药科大学生物制药学院，南京 210009

【正文语种】中文