低能氮离子注入和紫外线复合诱变选育高产酿酒酵母
第35卷 第2期
辐射研究与辐射工艺学报 J. Radiat. Res. Radiat. Process. V ol.35, No.2 2017年4月 https://www.wendangku.net/doc/8214475160.html,/fushe/CN/volumn/home.shtml April 2017
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基金资助：国家高技术研究发展计划（863计划）(2012AA023400)
第一作者：虞龙，男，1970年2月出生，2002年于中科院获得博士学位，教授，E-mail: lzsswgc@https://www.wendangku.net/doc/8214475160.html,
收稿日期：初稿2016-11-9；修回2017-1-6 Supported by the National High-tech Research Development Plan of China (863 Program, No. 2012AA023400)
First author: YU Long (male) was born in February 1970, and received his doctor’s degree from Chinese Academy of Science in 2002, professor, E-mail: lzsswgc@https://www.wendangku.net/doc/8214475160.html, Received 9 November 2016; accepted 6 January 2017
低能氮离子注入和紫外线复合诱变
选育高产酿酒酵母
虞 龙 姚驰亚 吴晓菲 李玉燕 张 娣 虞沐尧
（南京工业大学 生物与制药工程学院 南京 210000）
摘要 通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株，根据存活率及突变率确定离子注入的诱变
条件为电荷18 keV ，剂量400×1014 cm ?2，紫外线辐射60 s 。在该条件下，诱变获得的菌株正突变率和致死率
都最高，诱变效果最好。在该条件下进行研究后获得目标菌株SC-N9015，酒精产量从14.65%提升到
15.53%。多次传代实验结果显示遗传性状稳定。研究表明，通过低能氮离子注入和紫外线联合诱变选育高产
酒精酵母效果显著。
关键词 酿酒酵母，低能氮离子注入，紫外线，辐射，诱变筛选
中图分类号 TL99, Q691.5
DOI : 10.11889/j.1000-3436.2017.rrj.35.020401
Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by
low-energy nitrogen ion implantation and UV rays
YU Long YAO Chiya WU Xiaofei LI Yuyan ZHANG Di YU Muyao
(College of Biotechnology and Pharmaceutical Engineering, Nanjing University of technology, Nanjing 210000, China )
ABSTRACT The ethanol-producing yeast Saccharomyces cerevisiae was induced by N + implantation with an
output power of 18 keV and a dose of 400×1014 cm ?2, depending on the survival and mutation rates. The UV exposure
time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A
mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain
was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the
mutations. The study shows that the combination of low energy N + implantation with UV rays is an efficient method
for mutation breeding of S. cerevisiae .
KEYWORDS Saccharomyces cerevisiae, Low energy N + implantation, UV rays, Irradiation, Mutation breeding
CLC TL99, Q691.5
乙醇(Ethanol)是一元醇类，是很多酒类的重要
成分。乙醇易燃易爆，是经常使用的燃料、溶剂和
消毒液[1]。为了解决温室效应导致的全球变暖问题
及碳排放量问题，传统化石能源相关的不可再生资源已经不能满足人类需求，乙醇作为替代燃料是一个研究热点[2]。尽管通过微生物发酵法获得的乙醇在整个乙醇生产行业中所占比例非常低，但由于以含量丰富的可再生植物[3]为原料进行发酵，同时酿万方数据